Spike Protein VP8* of Human Rotavirus Recognizes Histo-Blood Group Antigens in a Type-Specific Manner

Huang, Pengwei; Xia, Ming; Tan, Ming; Zhong, Weiming; Wei, Chao; Wang, Leyi; Morrow, Ardythe L.; Jiang, Xi

doi:10.1128/jvi.05507-11

Cited by 236 publications

(370 citation statements)

References 63 publications

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“…This process of "humanization" following zoonotic transmission may further proceed generating new virus reassortants, as was shown in two distinct G8P [8] and G8P [4] rotaviruses reported in 2006 and 2009 in Europe, showing partial or little similarity with the DRC strains and close phylogenetic links with other common human rotavirus circulating in Europe belonging to G types other than G8 [38,39]. One of these latter strains, G8P [8] with a full Wa-like genome, unexpectedly became predominant among children with severe gastroenteritis in Croatia in 2006, suggesting that its emergence was [36,38] favored by an unusual gene repertoire [13,17,36].…”

Section: Rotavirus Zoonotic Transmissionmentioning

confidence: 94%

“…Also because of improved sequencing and whole genome genotyping analysis of rotavirus to explore rotavirus evolution, it is now possible to identify emerging zoonotic strains with a possible potential for rapid global spread, also in consideration of an increasing herd immunity by extending vaccination [13]. Using these approach, G8P [8] and G8P [6] strains identified in children with diarrhea in the Democratic Republic of Congo in 2003 [36] could be studied in detail, demonstrating for 9 of their genes a close evolutionary relationship with rotavirus strains belonging to the DS1-like (G2P [4]) sub-group, and suggesting at least three, and possibly four, consecutive reassortment events involving both DS1-like and Wa-like human rotaviruses and more animal strains of bovine (G8) and swine origin.…”

Section: Rotavirus Zoonotic Transmissionmentioning

confidence: 99%

“…For VP7 (G-genotyping), the protocol encompasses primers for common human rotavirus G-types G1-4, and G9, and emerging strains G8, G10, and G12. For VP4-typing, the multiplex assay covers the two common human P-types P [4] and P [8], and types P [6], and P[9-11] of possible animal origin. For special necessities, a full-genome typing approach may better help understand the evolution and occurrence of reassortment events of rotaviruses [14], although this is of hard applicability in the presence of multiple viral strains.…”

Section: Rotavirus Diagnostics In Environmental Samplesmentioning

confidence: 99%

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Vaccine preventable viral diseases and risks associated with waterborne transmission

Ruggeri

Fiore

2012

Annali Dell'Istituto Superiore Di Sanità

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Abstract. Rotavirus and poliovirus are paradigmatic viruses for causing major diseases affecting the human population. The impact of poliovirus is remarkably diminished because of vaccination during the last half century. Poliomyelitis due to wild polio currently affects a limited number of countries, and since 2000 sporadic outbreaks have been associated to neurovirulent vaccine-derived polioviruses. Conversely, rotavirus is presently very diffuse, accounting for the largest fraction of severe gastroenteritis among children <5 years-old. Vaccination towards rotavirus is still in its dawn, and zoonotic strains contribute to the emergence and evolution of novel strains pathogenic to man. The environment, particularly surface water, is a possible vehicle for large transmission of both viruses, but environmental surveillance of circulating strains can help promptly monitor entry of new virulent strains into a country, their shedding and spread.Key words: rotavirus, poliovirus, vaccine, waterborne transmission, surveillance. Riassunto (Malattie virali prevenibili con la vaccinazione e rischi associati alla trasmissione idrica).Rotavirus e poliovirus sono virus paradigmatici nel causare malattie importanti della popolazione umana. L'impatto dei poliovirus è diminuito marcatamente durante mezzo secolo di vaccinazione. La poliomielite dovuta a polio selvaggio affligge solo un limitato numero di paesi, e dal 2000 epidemie sporadiche sono state associate a poliovirus nerurovirulenti vaccino-derivati. Al contrario, rotavirus è oggi molto diffuso, essendo implicato nella maggiore parte dei casi di gastroenterite grave nei bambini di meno di 5 anni. La vaccinazione contro i rotavirus è ancora agli albori, e ceppi zoonotici contribuiscono all'emergenza ed evoluzione di nuovi ceppi patogeni per l'uomo. L'ambiente, in particolare le acque superficiali, è un possibile veicolo per la trasmissione di entrambi i virus, ma la sorveglianza ambientale dei ceppi circolanti può aiutare a monitorare prontamente l'ingresso di nuovi ceppi virulenti in un paese, il loro rilascio e diffusione.

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Section: Rotavirus Zoonotic Transmissionmentioning

confidence: 94%

Section: Rotavirus Zoonotic Transmissionmentioning

confidence: 99%

Section: Rotavirus Diagnostics In Environmental Samplesmentioning

confidence: 99%

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Vaccine preventable viral diseases and risks associated with waterborne transmission

Ruggeri

Fiore

2012

Annali Dell'Istituto Superiore Di Sanità

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“…Rotaviruses are classified into G and P types based on the composition of the outer capsid proteins VP7 and VP4, respectively. Binding to HBGA occurs through the VP8* domain of protein VP4 [76,77]. To date, 47 VP4 (P) genotypes have been identified, of which P[4], P[6] and P[8] are most common.…”

Section: Genetic Risk Factorsmentioning

confidence: 99%

Causes of Impaired Oral Vaccine Efficacy in Developing Countries

et al. 2017

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Oral vaccines are less immunogenic when given to infants in low-income compared with high-income countries, limiting their potential public health impact. Here, we review factors that might contribute to this phenomenon, including transplacental antibodies, breastfeeding, histo blood group antigens, enteric pathogens, malnutrition, microbiota dysbiosis and environmental enteropathy. We highlight several clear risk factors for vaccine failure, such as the inhibitory effect of enteroviruses on oral poliovirus vaccine. We also highlight the ambiguous and at times contradictory nature of the available evidence, which undoubtedly reflects the complex and interconnected nature of the factors involved. Mechanisms responsible for diminished immunogenicity may be specific to each oral vaccine. Interventions aiming to improve vaccine performance may need to reflect the diversity of these mechanisms. Enteric pathogens are a substantial threat to public health. This threat takes many forms, from the toxin-producing bacteria (e.g., Vibrio cholerae) and flagellated protozoa (e.g., Giardia lamblia) that colonize the mucosal surface of the small intestine to the enteroviruses that are capable of spreading via the bloodstream to the CNS, causing acute flaccid paralysis (e.g., poliovirus). Each year, enteric pathogens cause hundreds of millions of cases of diarrhea and approximately 800,000 deaths, the vast majority of which occur among children in low-income countries [1]. The most common causes of childhood diarrheal morbidity include rotavirus, Cryptosporidium, enterotoxigenic Escherichia coli and Shigella [2].Vaccines against enteric pathogens are important tools for mitigating this disease burden. Oral vaccines offer several distinct benefits compared with parenteral vaccines. They can be produced in large quantities at relatively low cost, are easy to administer and have the capacity to induce local immunity in the intestinal mucosa, thereby protecting vaccinated individuals against subsequent infection and -by blocking onward transmission -enhancing herd immunity [3,4]. Currently licensed oral vaccines include live-attenuated poliovirus vaccines containing one, two or three serotypes, live-attenuated rotavirus vaccines (Rotarix R , RotaTeq R , Lanzhou lamb rotavirus vaccine [China only] and Rotavac R [India only]), live-attenuated cholera vaccine (CVD 103-HgR or Vaxchora TM ), inactivated cholera vaccines (Dukoral R and Shanchol R ) and live-attenuated typhoid vaccine (Ty21a). Other oral vaccines are in development, including those against Shigella, enterotoxigenic E. coli, Campylobacter and Clostridium difficile, as well as several novel rotavirus vaccine candidates (including the human neonatal vaccine RV3-BB [5] and an oral bovine pentavalent vaccine [6]).Yet oral vaccines have an Achilles' heel -their immunogenicity and efficacy is impaired in low-income countries that experience the greatest burden of enteric disease (Box 1). For example, Rotarix has a 1-year protective efficacy against severe rotavirus-associated gas...

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“…It was believed that VP8* recognized either terminal sialic acid or internal sialic acid, mainly based on crystallographic and NMR studies (45)(46)(47)(48). However, recently a human strain (HAL1166) with a P [14] VP8* was found to bind to A-type histo-blood group antigen (49), a neonatal strain with a P [11] VP8* bound to type 2 precursor glycans (50), and several other P types recognized secretorrelated antigens Lewis b and H type 1 (51). These studies indicate that sialic acid might not be required by all RVs and that the glycan receptors are genotype-dependent.…”

mentioning

confidence: 99%

Human Milk Contains Novel Glycans That Are Potential Decoy Receptors for Neonatal Rotaviruses

Lasanajak

Song

et al. 2014

Molecular & Cellular Proteomics

117

126

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Human milk contains a rich set of soluble, reducing glycans whose functions and bioactivities are not well understood. Because human milk glycans (HMGs) have been implicated as receptors for various pathogens, we explored the functional glycome of human milk using shotgun glycomics. The free glycans from pooled milk samples of donors with mixed Lewis and Secretor phenotypes were labeled with a fluorescent tag and separated via multidimensional HPLC to generate a tagged glycan library containing 247 HMG targets that were printed to generate the HMG shotgun glycan microarray (SGM). To investigate the potential role of HMGs as decoy receptors for rotavirus (RV), a leading cause of severe gastroenteritis in children, we interrogated the HMG SGM with recombinant forms of VP8* domains of the RV outer capsid spike protein VP4 from human neonatal strains N155 (G10P[11]) and RV3(G3P[6]) and a bovine strain, B223 (G10P[11]). Glycans that were bound by RV attachment proteins were selected for detailed structural analyses using metadataassisted glycan sequencing, which compiles data on each glycan based on its binding by antibodies and lectins before and after exo-and endo-glycosidase digestion of the SGM, coupled with independent MS n analyses. These complementary structural approaches resulted in the identification of 32 glycans based on RV VP8* binding, many of which are novel HMGs, whose detailed structural assignments by MS n are described in a companion report. Although sialic acid has been thought to be important as a surface receptor for RVs, our studies indicated that sialic acid is not required for binding of glycans to individual VP8* domains. Remarkably, each VP8* recognized specific glycan determinants within a unique subset of related glycan structures where specificity differences arise from subtle differences in glycan structures. Molecular & Cellular Proteomics 13:

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Spike Protein VP8* of Human Rotavirus Recognizes Histo-Blood Group Antigens in a Type-Specific Manner

Cited by 236 publications

References 63 publications

Vaccine preventable viral diseases and risks associated with waterborne transmission

Vaccine preventable viral diseases and risks associated with waterborne transmission

Causes of Impaired Oral Vaccine Efficacy in Developing Countries

Human Milk Contains Novel Glycans That Are Potential Decoy Receptors for Neonatal Rotaviruses

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