Spike recognition and neutralization of SARS-CoV-2 Omicron subvariants elicited after the third dose of mRNA vaccine

Tauzin, Alexandra; Nicolas, Alexandre; Ding, Shilei; Benlarbi, Mehdi; Medjahed, Halima; Chatterjee, Debashree; Dionne, Katrina; Gong, Shang Yu; Gendron‐Lepage, Gabrielle; Bo, Yuxia; Perreault, Josée; Goyette, Guillaume; Gokool, Laurie; Arlotto, Pascale; Morrisseau, Chantal; Tremblay, Cécile; Martel-Laferrière, Valérie; Serres, Gaston De; Levade, Inès; Kaufmann, Daniel E.; Côté, Marceline; Bazin, Renée; Finzi, Andrés

doi:10.1016/j.celrep.2023.111998

Cited by 20 publications

(38 citation statements)

References 42 publications

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“…For the BQ.1.1 S, at the M4-Va3 timepoint donors with recent BTI better recognized the S than individuals with no recent infection, and this difference in recognition was more significant four weeks after the fourth dose ( Figure 1 C). The level of BQ.1.1 S recognition was significantly lower compared to D614G S in donors without recent BTI ( Figure 1 D), in agreement with recent reports [ 4 , 5 ]. In donors who had recently been infected, there was a significant but smaller difference in the level of recognition between the two Spikes compared to the other group.…”

Section: Resultssupporting

confidence: 92%

“…Pre-pandemic plasma samples were used as negative controls in cytometry assays (data not shown). The conformationally independent S2-specific monoclonal antibody CV3-25 was used as a positive control and to normalize Spike expression in flow cytometry assays, as described [ 4 , 26 , 27 , 28 , 29 ]. Alexa Fluor-647-conjugated goat anti-human antibodies (Abs) able to detect all Ig isotypes (anti-human IgM, IgG, IgA; Jackson ImmunoResearch Laboratories, Cat # 109-605-064) were used as secondary Abs to detect plasma binding in flow cytometry experiments.…”

Section: Methodsmentioning

confidence: 99%

“…The plasmids encoding the SARS-CoV-2 D614G and BQ.1.1 Spike variants were previously described [ 4 ]. The pNL4.3 R-E-Luc plasmid was obtained from the NIH AIDS Reagent Program (Cat# 3418).…”

Section: Methodsmentioning

confidence: 99%

“…Samples were acquired on a LSRFortessa cytometer (BD Biosciences, Franklin Lakes, NJ, USA), and data analysis was performed using FlowJo v10.7.1 (Tree Star). The conformationally independent anti-S2 antibody CV3-25, effective against all Spike variants, was used to normalize Spike expression, as reported [ 4 , 26 , 28 , 29 ]. The Median Fluorescence intensities (MFI) obtained with plasma were normalized to the MFI obtained with CV3-25 and presented as percentage of CV3-25 binding.…”

Section: Methodsmentioning

confidence: 99%

“…The Omicron BQ.1.1 variant is a sublineage of the BA.5 variant that spreads very rapidly and is now the major circulating lineage in several countries [ 1 , 2 ]. Recent studies have shown that original SARS-CoV-2 mRNA vaccines, based on the ancestral Wuhan strain Spike (S), lead to poor humoral responses against several Omicron subvariants, including the BQ.1.1 variant [ 3 , 4 , 5 ]. With the goal to improve immune responses against these subvariants, Moderna and Pfizer bivalent vaccines have recently been approved by health authorities in many countries [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Humoral Responses against BQ.1.1 Elicited after Breakthrough Infection and SARS-CoV-2 mRNA Vaccination

et al. 2023

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The Omicron BQ.1.1 variant is now the major SARS-CoV-2 circulating strain in many countries. Because of the many mutations present in its Spike glycoprotein, this variant is resistant to humoral responses elicited by monovalent mRNA vaccines. With the goal to improve immune responses against Omicron subvariants, bivalent mRNA vaccines have recently been approved in several countries. In this study, we measure the capacity of plasma from vaccinated individuals, before and after a fourth dose of mono- or bivalent mRNA vaccine, to recognize and neutralize the ancestral (D614G) and the BQ.1.1 Spikes. Before and after the fourth dose, we observe a significantly better recognition and neutralization of the ancestral Spike. We also observe that fourth-dose vaccinated individuals who have been recently infected better recognize and neutralize the BQ.1.1 Spike, independently of the mRNA vaccine used, than donors who have never been infected or have an older infection. Our study supports that hybrid immunity, generated by vaccination and a recent infection, induces higher humoral responses than vaccination alone, independently of the mRNA vaccine used.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Humoral Responses against BQ.1.1 Elicited after Breakthrough Infection and SARS-CoV-2 mRNA Vaccination

et al. 2023

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Understanding the landscape of the SARS‐CoV‐2‐specific T cells post‐omicron surge

Binayke,

Zaheer,

Vishwakarma

et al. 2024

Journal of Medical Virology

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Emerging evidence shows increased humoral response post‐omicron surge, but research on T cell responses is limited. This study investigated the durability, magnitude, and breadth of SARS‐CoV‐2‐spike‐specific T cell responses in 216 two‐dose vaccinated individuals pre‐ and post‐omicron surge. Post‐surge samples showed enhanced T cell responses, indicating widespread asymptomatic exposure to omicron. Further analysis of 105 individuals with multiple exposures to SARS‐CoV‐2 through boosters or infections showed that post‐omicron, two‐dose vaccinated individuals had T cell responses comparable to those of COVID‐19 convalescents or boosted individuals. Additionally, we report cross‐reactive T cell responses against omicron sub‐variants, including BA2.86, remained strong, with preserved frequencies of spike‐specific stem‐cell‐like memory T cells. In silico prediction indicates that mutated epitopes of JN.1 and KP.2 retain over 95.6% of their HLA binding capability. Overall, our data suggests that T cell responses are sustained, enhanced, and cross‐reactive against emerging SARS‐CoV‐2 variants following symptomatic or asymptomatic omicron infection.

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Physicochemical effects of emerging exchanges on the spike protein's RBM of the SARS-CoV-2 Omicron subvariants BA.1-BA.5 and its influence on the biological properties and attributes developed by these subvariants

Pondé

2023

Virology

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Spike recognition and neutralization of SARS-CoV-2 Omicron subvariants elicited after the third dose of mRNA vaccine

Cited by 20 publications

References 42 publications

Humoral Responses against BQ.1.1 Elicited after Breakthrough Infection and SARS-CoV-2 mRNA Vaccination

Humoral Responses against BQ.1.1 Elicited after Breakthrough Infection and SARS-CoV-2 mRNA Vaccination

Understanding the landscape of the SARS‐CoV‐2‐specific T cells post‐omicron surge

Physicochemical effects of emerging exchanges on the spike protein's RBM of the SARS-CoV-2 Omicron subvariants BA.1-BA.5 and its influence on the biological properties and attributes developed by these subvariants

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