Arboviruses belonging to the genera Flavivirus and
Alphavirus were detected in mosquitoes in a rural area of San
Bernardo del Viento (Córdoba, Colombia). A total of 22,180 mosquitoes were collected,
sorted into 2,102 pools, and tested by generic/nested reverse
transcription-polymerase chain reaction. Venezuelan equine encephalitis virus, dengue
virus, West Nile virus, St. Louis encephalitis virus, yellow fever virus, and
Culex flavivirus were detected and identified by sequencing. The
detection of arboviral pathogens in this zone represents possible circulation and
indicates a human health risk, demonstrating the importance of virological
surveillance activities.