Spin coated chitin films for biosensors and its analysis are dependent on chitin-surface interactions

Accelerated pharmaceutical protein development with integrated cell free expression, purification, and bioconjugation

“…Most likely an overcrowded peptide surface is less optimal for a functionally folded intein to form after split-intein mediated capture. During validation experiments of an earlier study (data not show) we found that for flat chitin-SPR sensors approximately 20% of chitin binding domain fused to Npu DnaE ΔC16 was an optimal distribution of the capture peptide 30 . One other limitation of the use of magnetic beads for light triggered release, is the difficulty to penetrate the magnetic bead slurry in the plastic wells of the KingFisher strips (ThermoFisher Scientific, USA) from above with UV-light.…”

“…2014) in a gradient-PCR (57°–62 °C) with 5′-tttaagaaggagatatacatATGGCGTTTACCGAACATTC-3′ and 5′-gtttcatagctgaggcaCATCTTCTTGTTGTTTGCG-3′ as forward and reverse primers, while the eGFP gene was isolated from pEGFP-C1 plasmid (Clontech, USA) in a PCR with 5′- tttaagaaggagatatacatATGGTGAGCAAGGGCGAG-3′ and 5′-gtttcatagctgaggcaCTTGTACAGCTCGTCCATGC-3′ as forward and reverse primers. The NpuDnaE N∆16 harbouring pET21a-CBD-NpuDnaE N∆16 plasmid 30 was linearized using 5′-TGCCTCAGCTATGAAACG-3′ and 5′-ATGTATATCTCCTTCTTAAAGTTAAAC-3′ as forward and reverse primers. The PCR products were assembled into plasmids pET21a-CNTF-NpuDnaE N∆16 and pET21a-eGFP-NpuDnaE N∆16 with NEBuilder®.…”

Recovery of chitin and chitosan from shrimp waste with microwave technique and versatile application

“…Chitin is a natural polymer with a crystalline structure highly organized, insoluble in aqueous solutions, and most of its solvents are organic with low chemical reactivity. Besides that, it is the second most abundant polysaccharide in nature (CASTELEIJN et al, 2018). Similarly, chitosan is a polysaccharide found widely in renewable sources, such as exoskeletons of crustaceans, insects, and fungal cell walls.…”

A conductive polymer composed of a cellulose-based flexible film and carbon nanotubes

“…Natural biomaterials with good structures and forms (such as silk broin, cellulose and chitin) are easy to process, and have adaptable performance, which makes them have potential in organic, electrical or ionic conductivities by combining with highly conductive materials or changing their structure through chemical modication. [4][5][6][7][8][9] Among them, the cellulose-based exible lm builds a bridge between natural biomaterials and exible bioelectronics due to its outstanding mechanical, exible and adaptable conductive properties. As a carrier material or a framework support material, the properties of the cellulose-based exible lm can be changed and expanded by in situ polymerization or in combination with conductive materials.…”