-In this study, we synthesized Ag2Se nanoparticles (NPs) in an aqueous solution and investigated the thermoelectric characteristics of Ag2Se NPs thin films on plastic substrates. Regardless of thermal annealing treatment, all the Ag2Se NPs thin films show the negative Seebeck coefficients, indicating the n-type characteristics. As the annealing temperature increases, the electric conductivity increases while the Seebeck coefficient decreases. The electric conductivity of the thin film annealed at 180°C is larger by 10 6 times, compared with the as-prepared thin film, And the maximum power density for the thin film annealed at 180°C is calculated to be 44 μW/cm 2 .Key Words : Thermoelectric, Silver selenide, Nanoparticles, Seebeck coefficient, Plastic substrate, Annealing