Spin-Coating-Based Facile Annular Photodynamic Intraocular Lens Fabrication for Efficient and Safer Posterior Capsular Opacification Prevention

Abstract

Posterior capsular opacification (PCO) is the most common complication after cataract surgery, which is primarily caused by the proliferation of the residual lens epithelial cells (LECs) in the lens capsule. Previous studies have demonstrated that a drug-eluting intraocular lens (IOL), aimed to in situ eliminate LECs, are an effective and promising way to prevent PCO. However, because of the potential toxicities of the antiproliferative drugs to the adjacent tissues, the safety of such drug-eluting IOLs is still a highly important issue to be solved. In this investigation, a facile photodynamic coating-modified IOL was developed for effective and safer PCO prevention. An annular poly(lactide-co-glycolic acid) (PLGA) coating loaded with photosensitizer chlorin e6 (Ce6) was prepared by a spin-coating technique. The optical property investigations showed that the Ce6@PLGA coating was particularly suitable for the IOL surface modification. The in vitro cell culture investigation showed that Ce6@PLGA coating-modified IOLs effectively eliminated LECs when treated with light illumination, whereas it appeared to have good cytocompatibility without irradiation. The investigation of the cell elimination mechanism showed that the apoptosis of HLECs may be associated with the cytomembrane disruption induced by ROS, which is generated by the photodynamic coating during light illumination. The in vivo implantation experiments confirmed the desired PCO prevention effect, as well as the safety to and biocompatibility with the surrounding tissues. Thus, the facile Ce6@PLGA coating will provide an effective yet safe alternative of IOL surface modification for PCO prevention.

Comparison of the Rate of Posterior Capsule Opacification Following Combined Treatment With Topical Dexamethasone 0.1% Plus Ketorolac 0.5% Eye Drops Versus Dexamethasone 0.1% Alone: A Two-Year, Randomized Clinical Investigation

“…Based on the findings from various in vivo and in vitro investigations in animals as well as in humans that NSAIDs can lessen the formation of PCO following cataract surgery [12,13], some new non-invasive clinical approaches to reduce after-cataract formation in humans are potentially important because complications from YAG capsulotomy after cataract surgery may result in significant loss of vision from cystoid macular edema, retinal breaks, and detachments with possible IOL damage or subluxation in some cases [1][2][3][4][5][6][7].…”

Non-viral gene therapy using RNA interference with PDGFR-α mediated epithelial-mesenchymal transformation for proliferative vitreoretinopathy

“…Immunofluorescence staining for Fibronectin and PDGFR-α of ocular tissues was also performed using the same method to assess EMT. Other tissues of the eye (cornea, iris) were evaluated by histological sections and HE staining to evaluate the safety of the treatment procedure [ 76 ].…”

Thermo-sensitive Poloxamer based antibacterial anti-inflammatory and photothermal conductive multifunctional hydrogel as injectable, in situ curable and adjustable intraocular lens

“…Patients recover their vision well after IOL implantation, yet they may also face postoperative problems such as posterior capsule opacification (PCO), infection and inflammation [ 6 , 7 ]. Many researchers, including ourselves, were focused on the improvement the IOLs materials with lower complication occurrence [ [8] , [9] , [10] , [11] , [12] ]. Besides, the improvement of ocular accommodation has also gained attentions due to the increasing demand for high-quality vision [ 13 ].…”