Spin-echo proton-nuclear-magnetic-resonance detection of normal and abnormal metabolites in plasma and urine

Iles, Richard A.; Buckingham, M. J.; Hawkes, Geoffrey E.

doi:10.1042/bst0110374

Cited by 26 publications

(11 citation statements)

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“…The intensive development of high resolution N M R in recent years has allowed its use for rapid multicomponent analysis of low-molecular-mass components in a wide variety of body fluids ( 1 ), including urine (2)(3)(4)(5)(6)(7)(8), plasma [ 9, l o ) , and cerebrospinal fluid (11,12). Proton N M R studies of urine have so far concentrated on highlighting changes in the excretion patterns of small molecules after changes in the physiological state of the donors, after organ failure, and during drug metabolism.…”

Section: Introductionmentioning

confidence: 99%

Effect of aging and diet on proton NMR spectra of rat urine

Bell¹,

Sadler²,

Morris³

et al. 1991

Magnetic Resonance in Med

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500 MHz 1H NMR studies of rat urine are used to assess metabolic changes resulting from aging (0.5-20 months) and changes in diet (casein versus chow). Aging rats fed chow diets decrease their excretion of citrate and 2-oxoglutarate, while the output of taurine and creatinine increases. Only young rats (1 month or less postweaning) excrete significant amounts of betaine and trimethylamine-N-oxide. Rats fed casein diets for 1 month postweaning do not excrete 2-oxoglutarate and excrete lower levels of hippurate, succinate, and citrate compared to rats fed chow diets. They also excrete N-methylnicotinamide. These high resolution proton NMR studies provide metabolic profiles which are not readily available by other techniques.

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Section: Introductionmentioning

confidence: 99%

Effect of aging and diet on proton NMR spectra of rat urine

Bell¹,

Sadler²,

Morris³

et al. 1991

Magnetic Resonance in Med

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“…The pioneering works on body fluids' analysis using NMR spectroscopy were published in the mid-1980s by the groups of J. K. Nicholson [32][33][34][35] and R. A. Iles [41][42][43][44][45].…”

Section: Non-localized Ex Vivo Nuclear Magnetic Resonance Spectroscop...mentioning

confidence: 99%

NMR Spectroscopy in Diagnosis and Monitoring of Methylmalonic and Propionic Acidemias

Deleanu,

Nicolescu

2024

Biomolecules

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Although both localized nuclear magnetic resonance spectroscopy (MRS) and non-localized nuclear magnetic resonance spectroscopy (NMR) generate the same information, i.e., spectra generated by various groups from the structure of metabolites, they are rarely employed in the same study or by the same research group. As our review reveals, these techniques have never been applied in the same study of methylmalonic acidemia (MMA), propionic acidemia (PA) or vitamin B12 deficiency patients. On the other hand, MRS and NMR provide complementary information which is very valuable in the assessment of the severity of disease and efficiency of its treatment. Thus, MRS provides intracellular metabolic information from localized regions of the brain, while NMR provides extracellular metabolic information from biological fluids like urine, blood or cerebrospinal fluid. This paper presents an up-to-date review of the NMR and MRS studies reported to date for methylmalonic and propionic acidemias. Vitamin B12 deficiency, although in most of its cases not inherited, shares similarities in its metabolic effects with MMA and it is also covered in this review.

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“…studies have been reported ofanimal serum and plasma (Nicholson, Buckingham & Sadler, 1983), and of serum, plasma and urine from humans (Iles, Buckingham & Hawkes, 1983;Nicholson, O'Flynn, Sadler, Macleod, Juul & Sonksen, 1984). For example, signals from the ketone bodies are readily detectable in serum, plasma and urine samples from fasting and diabetic subjects (Nicholson et al 1984), and unusual metabolites can be observed in urine samples from patients with inborn errors of metabolism (Iles et al 1983). Although n.m.r.…”

Section: Applicationsmentioning

confidence: 99%

Untitled

1986

Exp Physiol

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Introduction 335 (2) The principles of n.m.r. 336 2.1 Metabolic studies 336 2.2 N.m.r. imaging 336 (3) 31P n.m.r. studies of metabolism 3.1 Metabolite levels and pH in the steady state 3.2 Metabolic changes 3.2.1 Ischaemia, anoxia and hypoxia 3.2.2 Work 341 3.3 Studies of tumours 3.4 Steady-state kinetics (4) 'H n.m.r. studies of metabolism 4.1 Technical considerations 4.2 Applications (5) 13C n.m.r. studies of metabolism (6) 19F studies 351 (7) Other nuclei (8) Localization techniques for in vivo spectroscopy 353 (9) Summary (10) References

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Spin-echo proton-nuclear-magnetic-resonance detection of normal and abnormal metabolites in plasma and urine

Cited by 26 publications

References 0 publications

Effect of aging and diet on proton NMR spectra of rat urine

Effect of aging and diet on proton NMR spectra of rat urine

NMR Spectroscopy in Diagnosis and Monitoring of Methylmalonic and Propionic Acidemias

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