Spin-labelling of DNA with hydrazine mustard spin label (HMSL)

Raikova, E.; Ivanov, Iván; Kaffalieva, D.; Demirov, Georgi; Raikov, Z.

doi:10.1016/0020-711x(82)90174-4

Cited by 11 publications

(6 citation statements)

References 5 publications

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“…419 Bifunctional cross-linking agents are also of limited specificity. 420,421 One solution is presented by the use of an aptamer. For example, the malachite green RNA aptamer (a 38-nucleotide sequence) is known to bind malachite green or closely related dyes such as tetramethylrosamine (TMR).…”

Section: Paramagnetic Probes For Dna and Rnamentioning

confidence: 99%

Paramagnetic Chemical Probes for Studying Biological Macromolecules

et al. 2022

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Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent years witnessed a great increase in the variety of probes for the study of biological macromolecules (proteins, nucleic acids, and oligosaccharides). This Review aims to provide a comprehensive overview of the existing paramagnetic chemical probes, including chemical synthetic approaches, functional properties, and selected applications. Recent developments have seen, in particular, a rapid expansion of the range of lanthanoid probes with anisotropic magnetic susceptibilities for the generation of structural restraints based on residual dipolar couplings and pseudocontact shifts in solution and solid state NMR spectroscopy, mostly for protein studies. Also many new isotropic paramagnetic probes, suitable for NMR measurements of paramagnetic relaxation enhancements, as well as EPR spectroscopic studies (in particular double resonance techniques) have been developed and employed to investigate biological macromolecules. Notwithstanding the large number of reported probes, only few have found broad application and further development of probes for dedicated applications is foreseen.

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Section: Paramagnetic Probes For Dna and Rnamentioning

confidence: 99%

Paramagnetic Chemical Probes for Studying Biological Macromolecules

et al. 2022

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“…592 Under the conditions of the DNA labeling, the nitroxyl group of 861 was lost, resulting in a DNA with no EPR signal. 592 This result was in sharp contrast to the nitrogen mustard spin-labeled compounds which were found 585 to alkylate the double-stranded DNA. Compound 861 was used also to spin label erythrocyte ghosts.…”

Section: G Nitroxyl (Aminoxyl) Analogsmentioning

confidence: 77%

“…582 In early studies 584 spin-labeled derivatives of nitrogen mustards were used to label DNA. The hydrazine mustard spin label 857 was reacted 585 with DNA, and the bioenvironment of the nitroxyl in the DNA was analyzed by EPR spectroscopy. Such EPR analysis revealed 585 that 857 is a base-specific reagent, alkylating preferentially guanine, as well as indicating the difference between the spectra of immobilized double-stranded DNA and the spectra of nonimmobilized single-stranded DNA.…”

Section: G Nitroxyl (Aminoxyl) Analogsmentioning

confidence: 99%

A Critical Appraisal of the Evolution ofN-Nitrosoureas as Anticancer Drugs

1997

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“…Early on, alkylating or acylating agents, such as compounds 13 45 and 14 46 (Figure 6, A), were used for spin labelling,47 but for the aforementioned reasons had very limited sequence selectivity. In an attempt to increase the selectivity, bifunctional cross‐linking agents, such as the hydrazine mustard spin label 16 ,48 the psoralene derivative 17 49 and the spin‐labelled cisplatin 18 ,50 were developed. Although these cross‐linking agents have a sequence selectivity that spans a few nucleotides, such as intra‐strand cross‐linking of the sequence GG for 18 , they still lack enough specificity to be generally applicable for SDSL.…”

Section: Post‐synthetic Spin Labellingmentioning

confidence: 99%

Site‐Directed Spin Labelling of Nucleic Acids

Shelke

Sigurdsson

2012

Eur J Org Chem

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The study of the structure and dynamics of the nucleic acids and their complexes with other biomolecules is the basis for understanding their functions. Electron paramagnetic resonance (EPR) spectroscopy is a biophysical technique that in recent years has been increasingly used to investigate nucleic acids. EPR studies require paramagnetic centre(s), usually nitroxide spin‐label(s) that are incorporated at specific sites in the nucleic acid by site‐directed spin labelling (SDSL). In the last few years, spin labels with improved spectroscopic properties have emerged and new SDSL techniques have been developed. This microreview describes SDSL of nucleic acids in the context of the three spin labelling strategies: post‐synthetic spin labelling, labelling during oligonucleotide synthesis and noncovalent labelling.

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Spin-labelling of DNA with hydrazine mustard spin label (HMSL)

Cited by 11 publications

References 5 publications

Paramagnetic Chemical Probes for Studying Biological Macromolecules

Paramagnetic Chemical Probes for Studying Biological Macromolecules

A Critical Appraisal of the Evolution ofN-Nitrosoureas as Anticancer Drugs

Site‐Directed Spin Labelling of Nucleic Acids

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