Spin labelling study of interfacial properties of egg-phosphatidylcholine liposomes as a function of cholesterol concentrations

Mirosavljević, Krunoslav; Noethig-Laslo, Vesna

doi:10.1016/j.chemphyslip.2008.07.006

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…1a and 1b, there are two general approaches for experimental determination of the diffusion coefficient: methods that measure the time-dependent behaviour of a large number (virtual continuum) of particles over relatively large regions of space and methods that involve observing the properties of individual particles over a small region of space. Experimental techniques belonging to the former category include pulsed gradient nuclear magnetic resonance (NMR) (Stejskal and Tanner 1965; Morris and Johnson Jr. 1992; Regan and Kuchel 2003), electron spin resonance (ESR) procedures (Regan and Kuchel 2003; Mirosavljević and Noethig-Laslo 2008), optical microscopy-based observation of light-absorbing or light-emitting solutes (Guo et al 2008; Lorén et al 2009), neutron scattering (Doster and Longeville 2007) and single- and multi-wavelength dynamic light scattering experiments (Peetermans et al 1987). Experimental methods belonging to the later category, i.e.…”

Section: Experimental Measurement Of Single Particle Motion Within Thmentioning

confidence: 99%

Effects of macromolecular crowding on intracellular diffusion from a single particle perspective

Hall

Hoshino

2010

Biophys Rev

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Compared to biochemical reactions taking place in relatively well-defined aqueous solutions in vitro, the corresponding reactions happening in vivo occur in extremely complex environments containing only 60–70% water by volume, with the remainder consisting of an undefined array of bio-molecules. In a biological setting, such extremely complex and volume-occupied solution environments are termed ‘crowded’. Through a range of intermolecular forces and pseudo-forces, this complex background environment may cause biochemical reactions to behave differently to their in vitro counterparts. In this review, we seek to highlight how the complex background environment of the cell can affect the diffusion of substances within it. Engaging the subject from the perspective of a single particle’s motion, we place the focus of our review on two areas: (1) experimental procedures for conducting single particle tracking experiments within cells along with methods for extracting information from these experiments; (2) theoretical factors affecting the translational diffusion of single molecules within crowded two-dimensional membrane and three-dimensional solution environments. We conclude by discussing a number of recent publications relating to intracellular diffusion in light of the reviewed material.

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Section: Experimental Measurement Of Single Particle Motion Within Thmentioning

confidence: 99%

Effects of macromolecular crowding on intracellular diffusion from a single particle perspective

Hall

Hoshino

2010

Biophys Rev

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“…TEMPO-stearate was used recently in the study of interfacial properties of phospholipid vesicles (liposomes) (Alves and Perić, 2006;Mirosavljević and Noethig-Laslo, 2008;Alves et al, 2008). It was found that a negative charge in the liposome composition influences the amount of water bound at the surface, as well as the dynamics of the nitroxide moiety located at the liposome surface.…”

Section: Introductionmentioning

confidence: 99%

On the surface properties of oleate micelles and oleic acid/oleate vesicles studied by spin labeling

Dejanović

Noethig-Laslo

Šentjurc

et al. 2011

Chemistry and Physics of Lipids

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“…10 Although the lipid constituents of liposomes were varied, the presence of cholesterol was essential for the stability of the liposomes.…”

Section: Introductionmentioning

confidence: 99%

“…It is known that presence of cholesterol in the liposomes influenced the polarity profile of liposomes, induced formation of liquid-ordered and liquiddisordered domains and increased the penetration depth of the water molecules into the liposome. 10,13,14 In order to get more information on the possible role of the adamantyl moiety in interactions with the liposomes that contain cholesterol and negative charge, two different types of adamantyl molecules: 3-(adamantan-1-yl)propan-1-aminium chloride ( Figure 1, A) and 1-hydroxyadamantan (Figure 1, B) were studied in this work. Compound A is the molecule in the form of salt in which the NH 3 + Cl -group and adamantane moiety are separated by three carbon atoms, while compound B contains only an -OH group.…”

Section: Introductionmentioning

confidence: 99%

Effect of Adamantyl Compounds on Dynamics of Spin Labelled Multilamellar Liposomes

Mirosavljević¹,

Matković²,

Mlinarić‐Majerski³

et al. 2014

Croat. Chem. Acta

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Abstract. Interactions of two adamantyl molecules: 3-(adamantan-1-yl)propan-1-aminium chloride (compound A) and 1-hydroxyadamantan (compound B) with multilayer liposomes composed of L-α-phosphatidylcholine, cholesterol and dicetylphosphate, spin labelled with n-doxylstearic acids (n = 5, 7, 10, 12, 16) were investigated. In the presence of aminium salt (A) or alcohol (B) the increased dynamics of 5-doxylstearic acids and 16-doxylstearic acids in the multilamellar liposomes were determined in the temperature range from 250 K to 295 K. The increased dynamics of 5-doxylstearic acids suggested that both adamantyl compounds influenced molecular motions in the interfacial region of the liposome. The increased motional properties of 16-doxylstearic acids, located in the core of the multilamellar liposomes, suggested that both adamantyl compounds induced the structural changes in the hydrophobic core as well.

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Spin labelling study of interfacial properties of egg-phosphatidylcholine liposomes as a function of cholesterol concentrations

Cited by 6 publications

References 23 publications

Effects of macromolecular crowding on intracellular diffusion from a single particle perspective

Effects of macromolecular crowding on intracellular diffusion from a single particle perspective

On the surface properties of oleate micelles and oleic acid/oleate vesicles studied by spin labeling

Effect of Adamantyl Compounds on Dynamics of Spin Labelled Multilamellar Liposomes

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