Spin Trapping: An Essential Tool for the Study of Diseases Caused by Oxidative Stress

Barriga-González, Germán; Olea‐Azar, Claudio; Zúñiga-López, María Carolina; Folch-Cano, Christian; Aguilera‐Venegas, Benjamín; Porcal, Williams; González, Mercedes; Cerecetto, Hugo

doi:10.2174/1568026615666150206155108

Cited by 15 publications

(7 citation statements)

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“…Spin trapping in cellular systems.-Spin traps have not only been applied in artificial sytems but also in biological and medical studies in vitro and in vivo. [426][427][428][429][430][431][432][433][434][435] Due to the complex composition of solutions and low radical production in such experiments, careful control and calibration experiments need to be performed for a quantitative interpretation of spin trapping ESR measurements. Particularly, when working with cells in physiological buffer solutions, Fenton type reactions often have to be controlled by using metal chelators to avoid strong background signals and artefacts.…”

Section: Applications Of Spin Traps and Spin Probes-h 2 Omentioning

confidence: 99%

Review—Quantification of Hydrogen Peroxide by Electrochemical Methods and Electron Spin Resonance Spectroscopy

Gulaboski

Mirčeski

Kappl

et al. 2019

J. Electrochem. Soc.

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Reactive oxygen species (ROS) exhibit different spatial and temporal distributions as well as concentrations in-and outside the cell, thereby functioning as signaling or pathogen-destroying molecules. Especially the ROS H 2 O 2 is important for the patho/physiological status of an organism. Electrochemistry (EM) and electron spin resonance (ESR)-based techniques allow quantification of H 2 O 2 in artificial and living systems, coping a concentration range from low nM up to mM. Working electrodes for EM are optimized by diverse modifications and, additionally, redox mediators are used. Ultramicroelectrodes allow scanning of single cells to spatially resolve and quantify extracellular H 2 O 2 in real-time. With ESR spectroscopy, • O 2¯, but not H 2 O 2 , can be directly determined by spin probes in-and outside of cells in suspensions. Monitoring H 2 O 2 requires formation of intermediate radicals, detectable with spin probes. Low μM [H 2 O 2 ] can thus be assessed specifically. Using suitable spin traps, in-vivo ESR and immuno-spin trapping can visualize different radicals at their respective production sites in small animals, organs and tissues. Here, the redox reaction cascades may interfere with cell metabolism. Optimization of all methods established for H 2 O 2 determination would be favorable to finally combine them for mutual validation. Thus, a deeper insight into cellular ROS metabolism can be obtained.

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Section: Applications Of Spin Traps and Spin Probes-h 2 Omentioning

confidence: 99%

Review—Quantification of Hydrogen Peroxide by Electrochemical Methods and Electron Spin Resonance Spectroscopy

Gulaboski

Mirčeski

Kappl

et al. 2019

J. Electrochem. Soc.

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“…However, the ability to detect ROS generation directly in vitro is limited due to analytical difficulties, attributable mainly to their very short half-life. The ESR spin-trapping technique employed in the present study is useful for direct measurement of the ROS generated in experimental models [28].…”

Section: Discussionmentioning

confidence: 99%

Singlet oxygen generated by a new nonthermal atmospheric pressure air plasma device exerts a bactericidal effect on oral pathogens

et al. 2019

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Oral diseases generally have certain bacteria associated with them. Non-thermal atmospheric pressure plasma (NTAP), generated at atmospheric pressure and room temperature, incorporates several molecules, including reactive oxygen species, that can inactivate various bacteria including oral pathogens. For this reason, several NTAP devices have been developed to treat oral diseases. Use of noble gases can enhance the bactericidal efficacy of NTAP, but this requires additional gas supply equipment. Therefore, a new NTAP device that employs ambient air as the working gas was developed. The device generates non-thermal atmospheric pressure air plasma. Here, the singlet oxygen (1 O 2) levels generated, their bactericidal effects on oral pathogens (Streptococcus mutans, Porphyromonas gingivalis, and Enterococcus faecalis), and the bacterial oxidative stress they imposed were measured. 1 O 2 generation in phosphatebuffered saline was assessed qualitatively using electron spin resonance (ESR) spectroscopy, and bactericidal efficacy was evaluated by counting of colony-forming units/mL. Bacterial oxidative stress was determined by measurement of hydrogen peroxide (H 2 O 2) and superoxide dismutase (SOD) activity. ESR indicated that the level of 1 O 2 increased significantly and time-dependently, and was inversely correlated with distance, but the bactericidal effects were correlated only with treatment time (not distance) as H 2 O 2 increased and SOD levels decreased, suggesting that the new device has potential applicability for treatment of oral disease.

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“…To perform the free radical detection in the parasitic medium, ESR spectroscopy was employed, using the technique of spin trapping. The spin-trap was DMPO, that can cross cell membranes and react with several centred carbon and oxygen free radicals [37][38][39]. A solution of DMSO was used as a control (no radical species were detected).…”

Section: The Trypanocidal Activity Of Substituted Amidocoumarinsmentioning

confidence: 99%

Evaluation of Trypanocidal and Antioxidant Activities of a Selected Series of 3-amidocoumarins

Moncada‐Basualto

Lapier

Maya

et al. 2018

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Spin Trapping: An Essential Tool for the Study of Diseases Caused by Oxidative Stress

Cited by 15 publications

References 0 publications

Review—Quantification of Hydrogen Peroxide by Electrochemical Methods and Electron Spin Resonance Spectroscopy

Review—Quantification of Hydrogen Peroxide by Electrochemical Methods and Electron Spin Resonance Spectroscopy

Singlet oxygen generated by a new nonthermal atmospheric pressure air plasma device exerts a bactericidal effect on oral pathogens

Evaluation of Trypanocidal and Antioxidant Activities of a Selected Series of 3-amidocoumarins

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