Spin Trapping of Nitric Oxide in Aqueous Solutions of Cigarette Smoke

Shinagawa, Kozo; Tokimoto, Takayuki; Shirane, Kotaro

doi:10.1006/bbrc.1998.9653

Cited by 23 publications

(12 citation statements)

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“…The ESR signal with a three-line spectrum characteristic of an NO radical, which was barely observed directly after bubbling the smoke of one cigarette, began rapidly increasing with time up to around 25 min after the last addition of ferrous ion Fe(II), and then slowly approached a peak value for 2 hours or longer as shown in a previous publication (Shinagawa et al, 1998).…”

Section: Resultssupporting

confidence: 68%

“…The materials and techniques used in the present study were the same as those reported previously (Shinagawa et al, 1998), unless otherwise specified. Briefly, the main-stream smoke of hilite, a brand name of cigarette made by the Japan Tobacco Industry Co. (JT), was bubbled into the spin trapping solution consisting of 3 ml phosphate-buffered solution (PBS: pH 7.4) + 400 µl of 50 mM MGD + 200 µl of 50 mM FeSO 4 (smoking period: 60 s; smoking speed: 580 ml/min), and quickly the other half volume (200 µl) of the 50 mM FeSO 4 solution was added.…”

Section: Methodsmentioning

confidence: 99%

“…Nitric oxide (NO) was originally characterized to be an air pollutant and has been recently found in cigarette smoke (Bokhoben et al, 1951;Norman et al, 1965;Arnold et al, 1977;Schievelbein, 1979;Komarov et al, 1993;Lai et al, 1994;Shinagawa et al, 1998). On the other hand, from studies on the endothelium-derived relaxing factor (EDRF) in the quite different field of cardioangiology (Furchgott et al, 1980;Ignarro et al, 1987Ignarro et al, , 1988Palmer et al, 1987Palmer et al, , 1988Furchgott, 1988) it has also been In the present study, we investigated the origin of NO production in aqueous solution bubbled with cigarette smoke by using the spin trapping technique and comparing the time course of the NO production with those of experiments using NaNO 2 , NaNO 3 solutions and S-nitroso-N-acetyl penicillamine (SNAP) solution pre-treated with various concentrations of H 2 O 2 in place of the cigarette smoke.…”

Section: Introductionmentioning

confidence: 99%

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Nitric Oxide Generation in Aqueous Solutions of Cigarette Smoke and Approaches to Its Origin

Tokimoto

Shinagawa²

2001

Biological Chemistry

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By using the ESR spin trapping technique with the N-methyl-D-glucamine dithiocarbamate (MGD)2-Fe(II) complex, the generation of nitric oxide (NO), a gaseous free radical, was observed in NO spin trapping solution bubbled with the filtered main-stream of cigarette smoke. The ESR signal with a three-line spectrum characteristic of an NO radical, which was not observed immediately after bubbling of smoke, started rapidly increasing with time up to around 25 min after the last addition of ferrous ions Fe(II), and then slowly approached a peak value dependent on the burned cigarette mass and on the smoking speed. The production of NO was, however, much affected by air oxidation and enhanced by the addition of ascorbic acid. A certain concentration of sodium nitrite (NaNO2) solution, in which nitrite NO2- is assumed as the main origin of the NO, mimicked closely the time course of NO generation resulting from the smoke of one cigarette. The cigarette smoke that was passed through alkaline pyrogallol solution as a deoxidizer; however, it exhibited an unchanged intensity of NO signal throughout the measurement. These results strongly suggest that NO would be gradually reproduced from NO2- in the reductive aqueous solution containing excess Fe(II) through NO2, which is initially formed and is concomitantly oxidized from NO in cigarette smoke.

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Section: Resultssupporting

confidence: 68%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nitric Oxide Generation in Aqueous Solutions of Cigarette Smoke and Approaches to Its Origin

Tokimoto

Shinagawa²

2001

Biological Chemistry

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“…Cigarette smoke is an aerosol of complex chemical composition containing both organic and inorganic compounds, of which 4,800 have been identified thus far (41). Both vapor phase and particulate phase of smoke are known to possess free radicals (27,42). Whereas the gas-phase radicals are generally short lived, the radicals in the particulate phase are relatively stable and consist of a hydroquinone, semiquinone, quinone complex (25); this complex is an active redox system capable of reducing molecular oxygen to produce superoxide, eventually leading to H 2 O 2 and hydroxyl radicals.…”

Section: Resultsmentioning

confidence: 99%

DNA Damage by Benzo(a)pyrene in Human Cells Is Increased by Cigarette Smoke and Decreased by a Filter Containing Rosemary Extract, Which Lowers Free Radicals

Alexandrov

Rojas²,

Rolando³

2006

Cancer Research

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We found previously that the human lung benzo(a)pyrene (BP)-7,8-diol-9,10-epoxide-N 2 -deoxyguanosine (BPDE-dG) adduct concentrate in the target bronchial cells. This adduct is now considered to be critical event in tumorigenesis by BP. In this study, we investigate the contribution of cigarette smoke on the BPDE-dG formation. In a cell-free system, the amount of (À)-anti-BPDE-dG adduct increased linearly with concentration of cigarette smoke in the presence of (+)-BP-7,8-diol. Catalase and superoxide dismutase inhibited its formation by >80%. When MCF-7 cells were treated for 2 hours with the (+)-BP-7,8-diol, cigarette smoke increased dose dependently the formation of (À)-anti-BPDE-dG and decreased the cytochrome P450 (CYP)-dependent formation of (+)-r-7,t-8-dihydroxy-c-9,10-oxy-7,8,9,10-tetrahydro-BP the adduct. Then, cells were treated for up to 1 day with BP and then exposed for 2 hours with cigarette smoke. During these 2 hours, there are twice the increase in the adduct formation in cells treated with cigarette smoke compared with levels in nontreated cells due to CYP activity. Thus, cigarette smoke containing reactive oxygen species may activate the second step of BP metabolic way, leading to the formation of BPDE-dG adduct. Cigarette smoke thus seems may be in part responsible for the formation of the critical lung tumorigenic adduct. Finally, modified cigarette filter containing rosemary extract decreases by >70% of the BPDE-dG adducts level due to the cigarette smoke in MCF-7 cells. This approach may lead to decreasing lung cancer risk in addicted smokers. (Cancer Res 2006; 66(24): 11938-45)

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“…Fresh cigarette smoke contains from 300 to 500 parts per million of nitric oxide ( Cueto & Pryor 1994). The nitric oxide radical is produced slowly from nitric oxide radical donors such as amine complexes, peroxinitrite and other reactants including nitrogen oxides ( Shinagawa et al . 1998 ).…”

Section: Free Radicals and Cigarette Smokementioning

confidence: 99%

Cigarette smoking, endothelial injury and cardiovascular disease

Pittilo

2000

Int J Experimental Path

207

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Despite the fact that the epidemiological evidence linking cigarette smoking with cardiovascular disease is overwhelming, the precise components of cigarette smoke responsible for this relationship and the mechanisms by which they exert their effect have not yet been elucidated. There are however, some promising pointers as a result of recent developments and this review concentrates on new evidence since earlier reviews of this topic. It is now known that the endothelium has a vastly more important role than was ever thought to be the case a decade ago. Its role in health and disease is increasingly understood, as is the relationship between endothelial injury and the development of atherosclerosis. There is considerable evidence that cigarette smoking can result in both morphological and biochemical disturbances to the endothelium both in vivo and in cell culture systems. Cigarette smoke is a complex mixture and only a few components have been extensively studied. Nicotine and carbon monoxide are much less damaging than is whole smoke. However the free radical components of cigarette smoke have been shown to cause damage in model systems. Further work will be necessary to consolidate the evidence base but the data reported in this review suggest that the free radical components of cigarette smoke may be responsible for the morphological and functional damage to endothelium that has been observed in model systems.

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Spin Trapping of Nitric Oxide in Aqueous Solutions of Cigarette Smoke

Cited by 23 publications

References 0 publications

Nitric Oxide Generation in Aqueous Solutions of Cigarette Smoke and Approaches to Its Origin

Nitric Oxide Generation in Aqueous Solutions of Cigarette Smoke and Approaches to Its Origin

DNA Damage by Benzo(a)pyrene in Human Cells Is Increased by Cigarette Smoke and Decreased by a Filter Containing Rosemary Extract, Which Lowers Free Radicals

Cigarette smoking, endothelial injury and cardiovascular disease

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