Spinosad-mediated effects on survival, overall group activity and the midgut of workers of Partamona helleri (Hymenoptera: Apidae)

Araújo, Renan dos Santos; Lopes, Mariana Calábria; Barbosa, Wagner Faria; Gonçalves, Wagner Gonzaga; Fernandes, Kenner Morais; Martins, Gustavo Ferreira; Tavares, Mara Garcia

doi:10.1016/j.ecoenv.2019.03.050

Cited by 33 publications

(16 citation statements)

References 39 publications

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“…(El et al, 2020). Exposure of Partamona helleri to spinosad caused morphological alterations in the midgut and intensified the processes of apoptosis, autophagy and oxidative stress in this organ (Araujo et al, 2019). While The spinosad LC50 caused disorganization of the epithelia of tested organs and induced oxidative stress and cell death on A. mellifera.…”

Section: Resultsmentioning

confidence: 98%

Biochemical and Histological Responses of Red Palm Weevil, Rhynchophorus ferrugineus Exposed to Sub-lethal Levels of Different Insecticide Classes

Ahmed

Elsobki

2021

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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Date palm Phoenix dactylifera L. is cultivated well especially in tropical and semitropical climates. It is considered an economic fruit crop in Egypt and the Middle East. Date palm fruits are a very good source of food with several medicinal qualities (Al-Dosary et al., 2016). Palms are infested by many harmful pests, but the red palm weevil is the most important of them at all (Salman et al., 2020). The red palm weevil (RPW), Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae), is the main borer attacking palm trees (Milosavljević et al., 2019). Most of the popular date palm cultivars are also suitable for RPW infestation (Sallam et al., 2012).The widespread RPW enabled it to expand its geographical range to extend to Asian, African, European and American countries, causing catastrophic damage to palm production

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Section: Resultsmentioning

confidence: 98%

Biochemical and Histological Responses of Red Palm Weevil, Rhynchophorus ferrugineus Exposed to Sub-lethal Levels of Different Insecticide Classes

Ahmed

Elsobki

2021

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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“…Butenyl-spinosyn is a highly effective, broad-spectrum, promising biological pesticide produced by the soil microbe Saccharopolyspora pogona. − The chemical modification of the butenyl-spinosyn structure can produce a series of derivatives, which provides abundant raw materials for its industrial development and utilization . The butenyl-spinosyn biosynthetic gene cluster and the genes associated with each synthesis step have been identified, and the genome of S.…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteomics Reveals the Effect of the Transcriptional Regulator Sp13016 on Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona

Tang

et al. 2021

J. Agric. Food Chem.

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Butenyl-spinosyn is a highly effective and broad-spectrum biopesticide produced by Saccharopolyspora pogona. However, the yield of this compound is difficult to increase because the regulatory mechanism of secondary metabolism is still unknown. Here, the transcriptional regulator Sp13016 was discovered to be highly associated with butenyl-spinosyn synthesis and bacterial growth. Overexpression of sp13016 improved butenyl-spinosyn production to a level that was 2.84-fold that of the original strain, while deletion of sp13016 resulted in a significant decrease in yield and growth inhibition. Comparative proteomics revealed that these phenotypic changes were attributed to the influence of Sp13016 on the central carbon metabolism pathway to regulate the supply of precursors. Our research helps to reveal the regulatory mechanism of butenyl-spinosyn biosynthesis and provides a reference for increasing the yield of natural products of Actinomycetes.

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“…It is structurally and chemically related to spinosyn produced by Saccharopolyspora spinosa [ 2 , 3 ]. Butenyl-spinosyn has excellent activity against various insects, such as Lepidoptera, Diptera, and Coleoptera, but has little or no effect on a broad range of nontarget insects and mammals, making it one of the most promising biological pesticides [ 4 , 5 ]. At present, the butenyl-spinosyn biosynthetic gene cluster ( bus cluster) and its biosynthetic pathway have been elaborated [ 2 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

A TetR family transcriptional regulator, SP_2854 can affect the butenyl-spinosyn biosynthesis by regulating glucose metabolism in Saccharopolyspora pogona

et al. 2022

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Background Butenyl-spinosyn produced by Saccharopolyspora pogona exhibits strong insecticidal activity and a broad pesticidal spectrum. Currently, important functional genes involve in butenyl-spinosyn biosynthesis remain unknown, which leads to difficulty in efficiently understanding its regulatory mechanism, and improving its production by metabolic engineering. Results Here, we identified a TetR family transcriptional regulator, SP_2854, that can positively regulate butenyl-spinosyn biosynthesis and affect strain growth, glucose consumption, and mycelial morphology in S. pogona. Using targeted metabolomic analyses, we found that SP_2854 overexpression enhanced glucose metabolism, while SP_2854 deletion had the opposite effect. To decipher the overproduction mechanism in detail, comparative proteomic analysis was carried out in the SP-2854 overexpressing mutant and the original strain, and we found that SP_2854 overexpression promoted the expression of proteins involved in glucose metabolism. Conclusion Our findings suggest that SP_2854 can affect strain growth and development and butenyl-spinosyn biosynthesis in S. pogona by controlling glucose metabolism. The strategy reported here will be valuable in paving the way for genetic engineering of regulatory elements in actinomycetes to improve important natural products production.

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Spinosad-mediated effects on survival, overall group activity and the midgut of workers of Partamona helleri (Hymenoptera: Apidae)

Cited by 33 publications

References 39 publications

Biochemical and Histological Responses of Red Palm Weevil, Rhynchophorus ferrugineus Exposed to Sub-lethal Levels of Different Insecticide Classes

Biochemical and Histological Responses of Red Palm Weevil, Rhynchophorus ferrugineus Exposed to Sub-lethal Levels of Different Insecticide Classes

Comparative Proteomics Reveals the Effect of the Transcriptional Regulator Sp13016 on Butenyl-Spinosyn Biosynthesis in Saccharopolyspora pogona

A TetR family transcriptional regulator, SP_2854 can affect the butenyl-spinosyn biosynthesis by regulating glucose metabolism in Saccharopolyspora pogona

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