Spinosad resistance of melon thrips, Thrips palmi, is conferred by G275E mutation in α6 subunit of nicotinic acetylcholine receptor and cytochrome P450 detoxification

Bao, Wen Xue; Narai, Yutaka; Nakano, Akio; Kaneda, Takemichi; Murai, Tamotsu; Sonoda, Shoji

doi:10.1016/j.pestbp.2014.04.013

Cited by 77 publications

(78 citation statements)

References 25 publications

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“…Because they have the same molecular target, a change in nAChR may result in a lower affinity to several products (Puinean et al, 2013; Bao et al, 2014). This consideration is also true for both pairs natural pyrethrum / pyrethroid (Soderlund and Knipple, 2003) and nicotinoid/neonicotinoids (Bodereau-Dubois et al, 2012).…”

Section: Resistance To Biocontrol Agentsmentioning

confidence: 99%

“…Many commercial products based on pyrethrum contain PBO to increase its effectiveness. Resistance to spinosad is attributed to detoxification by p450 in the following pests: Musca domestica (Hojland et al, 2014), Thrips palmi (Bao et al, 2014), Cydia pomonella (Reyes and Sauphanor, 2008), Plutella xylostella (Pu et al, 2010), Bemisia tabaci (Wang et al, 2009a), and Helicoverpa armigera (Wang et al, 2009b). …”

Section: Resistance To Biocontrol Agentsmentioning

confidence: 99%

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Resistance to bio-insecticides or how to enhance their sustainability: a review

Siegwart

Graillot

Lopez³

et al. 2015

Front. Plant Sci.

153

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After more than 70 years of chemical pesticide use, modern agriculture is increasingly using biological control products. Resistances to conventional insecticides are wide spread, while those to bio-insecticides have raised less attention, and resistance management is frequently neglected. However, a good knowledge of the limitations of a new technique often provides greater sustainability. In this review, we compile cases of resistance to widely used bio-insecticides and describe the associated resistance mechanisms. This overview shows that all widely used bio-insecticides ultimately select resistant individuals. For example, at least 27 species of insects have been described as resistant to Bacillus thuringiensis toxins. The resistance mechanisms are at least as diverse as those that are involved in resistance to chemical insecticides, some of them being common to bio-insecticides and chemical insecticides. This analysis highlights the specific properties of bio-insecticides that the scientific community should use to provide a better sustainability of these products.

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Section: Resistance To Biocontrol Agentsmentioning

confidence: 99%

Section: Resistance To Biocontrol Agentsmentioning

confidence: 99%

Resistance to bio-insecticides or how to enhance their sustainability: a review

Siegwart

Graillot

Lopez³

et al. 2015

Front. Plant Sci.

153

View full text Add to dashboard Cite

show abstract

“…spinosad) mediated by target‐site mutation point to an insect nAChRα6 subunit. These alterations of insect nAChRα6 genes can be grouped into (i) point mutation (G275E mutation from Frankliniella occidentalis , Thrips palmi and Tuta absoluta , P146S mutation from D. melanogaster ), (ii) disruption mutation (null or chimeric mutation of Drosophila melanogaster ), (iii) deletion mutation of P. xylostella , (iv) truncated mutation of P. xylostella , F. occidentalis and Bactrocera dorsalis , and (v) exon skipping of T. absoluta . Among all these cases of spinosad resistance, either point mutation or disruption mutation identified in D. melanogaster nAChRα6 have been validated by in vivo or in vitro approaches.…”

Section: Introductionmentioning

confidence: 99%

Disruption of nicotinic acetylcholine receptor α6 mediated by CRISPR/Cas9 confers resistance to spinosyns in Plutella xylostella

Wang

et al. 2019

Pest Management Science

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BACKGROUND The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system provides some advantages over other reverse genetic techniques to investigate the causal relationship between insecticide resistance phenotype and candidate gene. Several studies published to date point to the nicotinic acetylcholine receptor (nAChR) α6 subunit strongly associated with spinosyns resistance in insects, including Plutella xylostella. However, reverse genetic verification of the P. xylostella nAChRα6 has not yet been achieved via an in vivo approach. RESULTS Here, we successfully constructed a homozygous strain (Pxα6‐KO) with a 2‐nt deletion mutation of nAChRα6 by CRISPR/Cas9 coupled with non‐homologous end joining approach in P. xylostella. The manipulated mutation results in a frame shift in the open reading frame of transcripts, which produces a predicted protein truncated in the TM3–TM4 loop region. When compared to the background strain IPP‐S, the knockout strain Pxα6‐KO exhibited 229‐ and 1462‐fold resistance to spinosad and spinetoram, respectively, but no or limited (resistance ratios <3‐fold) effects on the toxicities of imidacloprid, abamectin, β‐cypermethrin, indoxacarb, metaflumizone and chlorantraniliprole. Furthermore, the mode of inheritance of the acquired spinetoram resistance was autosomal recessive and significantly linked with the 2‐nt deletion mutation of nAChRα6 in the Pxα6‐KO strain. CONCLUSION In vivo functional investigation demonstrates the causality of the Pxα6 truncating mutation with high levels of resistance to spinosyns in P. xylostella. Our results suggest the Pxα6‐KO strain underlies an autosomal, recessive mode of inheritance for spinetoram resistance, and reinforces the association of this gene to the mode of action of spinosyns. © 2019 Society of Chemical Industry

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“…Recently a single point mutation (G275E) in the Frankliniella occidentalis (Western flower thrip) orthologue, Foa6, was reported to be associated with >350,000-fold spinosad resistance in a laboratoryselected strain and detected in a field collected population (Puinean et al, 2013). It has also been associated with field resistance to spinosad in the melon thrip, Thrips palmi (Bao et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Expression of insect α6-like nicotinic acetylcholine receptors in Drosophila melanogaster highlights a high level of conservation of the receptor:spinosyn interaction

Perry

Somers

Yang

et al. 2015

Insect Biochemistry and Molecular Biology

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Insecticide research has often relied on model species for elucidating the resistance mechanisms present in the targeted pests. The accuracy and applicability of extrapolations of these laboratory findings to field conditions varies but, for target site resistance, conserved mechanisms are generally the rule rather than the exception (Perry et al., 2011). The spinosyn class of insecticides appear to fit this paradigm and are a pest control option with many uses in both crop and animal protection. Resistance to spinosyns has been identified in both laboratory-selected and field-collected pest insects. Studies using the model insect, Drosophila melanogaster, have identified the nicotinic acetylcholine receptor subunit, Dα6 as an important target of the insecticide spinosad (Perry et al., 2007; Watson et al., 2010). Field-isolated resistant strains of several agricultural pest insects provide evidence that resistance cases are often associated with mutations in orthologues to Dα6 (Baxter et al., 2010; Puinean et al., 2013). The expression of these receptors is difficult in heterologous systems. In order to examine the biology of the Dα6 receptor subunit further, we used Drosophila as a model and developed an in vivo rescue system. This allowed us to express four different isoforms of Dα6 and show that each is able to rescue the response to spinosad. Regulatory sequences upstream of the Dα6 gene able to rescue the resistance phenotype were identified. Expression of other D. melanogaster subunits revealed that the rescue phenotype appears to be Dα6 specific. We also demonstrate that expression of pest insect orthologues of Dα6 from a variety of species are capable of rescuing the spinosad response phenotype, verifying the relevance of this receptor to resistance monitoring in the field. In the absence of a robust heterologous expression system, this study presents an in vivo model that will be useful in analysing many other aspects of these receptors and their biology.

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Spinosad resistance of melon thrips, Thrips palmi, is conferred by G275E mutation in α6 subunit of nicotinic acetylcholine receptor and cytochrome P450 detoxification

Cited by 77 publications

References 25 publications

Resistance to bio-insecticides or how to enhance their sustainability: a review

Resistance to bio-insecticides or how to enhance their sustainability: a review

Disruption of nicotinic acetylcholine receptor α6 mediated by CRISPR/Cas9 confers resistance to spinosyns in Plutella xylostella

Expression of insect α6-like nicotinic acetylcholine receptors in Drosophila melanogaster highlights a high level of conservation of the receptor:spinosyn interaction

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