Spinster Controls Dpp Signaling during Glial Migration in the<i>Drosophila</i>Eye

Yuva-Aydemir, Yeliz; Bauke, Ann-Christin; Klämbt, Christian

doi:10.1523/jneurosci.0459-11.2011

Cited by 31 publications

(31 citation statements)

References 53 publications

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“…Proliferation of glial progenitor cells occurs in an FGF‐, Merlin/Hippo‐ and Decapentaplegic‐dependent manner (Franzdóttir et al, ; Rangarajan, Courvoisier, & Gaul, ; Reddy & Irvine, ; Sieglitz et al, ; Yuva‐Aydemir, Bauke, & Klämbt, ). During glial migration, the perineurial FGF‐receptor Htl is activated at low levels by the FGF‐8 like ligand Pyramus which is secreted by the subperineurial glia (Franzdóttir et al, ; Sieglitz et al, ).…”

Section: Optic Nerve Modelmentioning

confidence: 99%

Drosophila glia: Few cell types and many conserved functions

Yildirim

Petri

Kottmeier

et al. 2018

Glia

147

153

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Glial cells constitute without any dispute an essential element in providing an efficiently operating nervous system. Work in many labs over the last decades has demonstrated that neuronal function, from action potential generation to its propagation, from eliciting synaptic responses to the subsequent postsynaptic integration, is evolutionarily highly conserved. Likewise, the biology of glial cells appears conserved in its core elements and therefore, a deeper understanding of glial cells is expected to benefit from analyzing model organisms such as Drosophila melanogaster. Drosophila is particularly well suited for studying glial biology since in the fly nervous system only a limited number of glial cells exists, which can be individually identified based on position and a set of molecular markers. In combination with the well‐known genetic tool box an unprecedented level of analysis is feasible, that not only can help to identify novel molecules and principles governing glial cell function but also will help to better understand glial functions first identified in the mammalian nervous system. Here we review the current knowledge on Drosophila glia to spark interest in using this system to analyze complex glial traits in the future.

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Section: Optic Nerve Modelmentioning

confidence: 99%

Drosophila glia: Few cell types and many conserved functions

Yildirim

Petri

Kottmeier

et al. 2018

Glia

147

153

View full text Add to dashboard Cite

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“…Fixation and treatment of tissues for immunohistochemistry was performed as described (Yuva-Aydemir et al, 2011). Anti-Repo antibodies (Halter et al, 1995;Lee and Jones, 2005) were obtained from the Developmental Studies Hybridoma Bank (Iowa City, IA, USA).…”

Section: Immunohistochemistry and Electron Microscopy Analysesmentioning

confidence: 99%

Axonal wrapping in theDrosophilaPNS is controlled by glia-derived neuregulin homolog Vein

et al. 2015

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Efficient neuronal conductance requires that axons are insulated by glial cells. For this, glial membranes need to wrap around axons. Invertebrates show a relatively simple extension of glial membranes around the axons, resembling Remak fibers formed by Schwann cells in the mammalian peripheral nervous system. To unravel the molecular pathways underlying differentiation of glial cells that provide axonal wrapping, we are using the genetically amenable Drosophila model. At the end of larval life, the wrapping glia differentiates into very large cells, spanning more than 1 mm of axonal length. The extension around axonal membranes is not influenced by the caliber of the axon or its modality. Using cell typespecific gene knockdown we show that the extension of glial membranes around the axons is regulated by an autocrine activation of the EGF receptor through the neuregulin homolog Vein. This resembles the molecular mechanism employed during cell-autonomous reactivation of glial differentiation after injury in mammals. We further demonstrate that Vein, produced by the wrapping glia, also regulates the formation of septate junctions in the abutting subperineurial glia. Moreover, the wrapping glia indirectly controls the proliferation of the perineurial glia. Thus, the wrapping glia appears center stage to orchestrate the development of the different glial cell layers in a peripheral nerve.

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“…Fixation and preparation of tissues for immunohistochemistry was performed as described (Yuva-Aydemir et al, 2011). Anti-Repo and antiCut antibodies were obtained from DSHB.…”

Section: Immunohistochemistry and Tem Analysesmentioning

confidence: 99%

A transcriptional network controlling glial development in theDrosophilavisual system

et al. 2015

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In the nervous system, glial cells need to be specified from a set of progenitor cells. In the developing Drosophila eye, perineurial glia proliferate and differentiate as wrapping glia in response to a neuronal signal conveyed by the FGF receptor pathway. To unravel the underlying transcriptional network we silenced all genes encoding predicted DNA-binding proteins in glial cells using RNAi. Dref and other factors of the TATA box-binding protein-related factor 2 (TRF2) complex were previously predicted to be involved in cellular metabolism and cell growth. Silencing of these genes impaired early glia proliferation and subsequent differentiation. Dref controls proliferation via activation of the Pdm3 transcription factor, whereas glial differentiation is regulated via Dref and the homeodomain protein Cut. Cut expression is controlled independently of Dref by FGF receptor activity. Loss-and gain-of-function studies show that Cut is required for glial differentiation and is sufficient to instruct the formation of membrane protrusions, a hallmark of wrapping glial morphology. Our work discloses a network of transcriptional regulators controlling the progression of a naïve perineurial glia towards the fully differentiated wrapping glia.

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Spinster Controls Dpp Signaling during Glial Migration in theDrosophilaEye

Cited by 31 publications

References 53 publications

Drosophila glia: Few cell types and many conserved functions

Drosophila glia: Few cell types and many conserved functions

Axonal wrapping in theDrosophilaPNS is controlled by glia-derived neuregulin homolog Vein

A transcriptional network controlling glial development in theDrosophilavisual system

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