Spiny mice activate unique transcriptional programs after severe kidney injury regenerating organ function without fibrosis

Okamura, Daryl M.; Brewer, Chris M.; Wakenight, Paul; Bahrami, Nadia M.; Bernardi, Kristina G.; Tran, Amy; Olson, Jill; Shi, Xiaogang; Yeh, Szu-Ying; Piliponsky, Adrian M.; Collins, Sarah; Nguyen, Elizabeth Dong; Timms, Andrew E.; MacDonald, James W.; Bammler, Theo K.; Nelson, Branden R.; Millen, Kathleen J.; Beier, David R.; Majesky, Mark W.

doi:10.1016/j.isci.2021.103269

Cited by 27 publications

(15 citation statements)

References 55 publications

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“…Tissues (blood, heart, liver, testis) were collected from an adult male Acomys cahirinus and homogenized, and RNA isolation, library generation, and sequencing were performed as previously described (Brewer et al 2021;Okamura et al 2021). Briefly, total RNA was extracted in Trizol solution (Ambion), DNase treated, and purified (PureLink RNA Mini Kit, Thermo Fisher Scientific).…”

Section: Rna Isolation and Mappingmentioning

confidence: 99%

“…Here, we report a long-read-based chromosome-level assembly for the African spiny mouse A. cahirinus, a member of the Acomys family that is known to be capable of organ regeneration (Brewer et al 2021;Okamura et al 2021). The assembled A. cahirinus genome is more contiguous and has more complete genes when compared to previously published reference genomes (Wang et al 2022), with an N50 of 127 Mb and BUSCO score of 98.5%.…”

Section: Introductionmentioning

confidence: 96%

“…The spiny mouse also demonstrates the ability to restore skeletal muscle after damage induced by cardiotoxin (Garry et al 2016;Maden et al 2018). These healing properties extend to internal organs; kidney damage induced using aggressive models of obstructive and ischemic injury is followed by complete regeneration of functional kidney tissue without scarring (Okamura et al 2021). The spiny mouse has also been shown to exhibit resistance to myocardial ischemia and minimal scarring, as well as improvement in cardiac function after injury (Qi et al 2021;Koopmans et al 2021;Peng et al 2021).…”

Section: Introductionmentioning

confidence: 99%

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GENOME REPORT: Chromosome-scale genome assembly of the African spiny mouse (Acomys cahirinus)

Nguyen

Fard

Kim

et al. 2023

Preprint

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There is increasing interest in the African spiny mouse (Acomys cahirinus) as a model organism because of its ability for regeneration of tissue after injury in skin, muscle, and internal organs such as the kidneys. A high-quality reference genome is needed to better understand these regenerative properties at the molecular level. Here, we present an improved reference genome forA. cahirinusgenerated from long Nanopore sequencing reads. We confirm the quality of our annotations using RNA sequencing data from four different tissues. Our genome is of higher contiguity and quality than previously reported genomes from this species and will facilitate ongoing efforts to better understand the regenerative properties of this organism.

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Section: Rna Isolation and Mappingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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GENOME REPORT: Chromosome-scale genome assembly of the African spiny mouse (Acomys cahirinus)

Nguyen

Fard

Kim

et al. 2023

Preprint

Self Cite

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“…The spiny mouse (Acomys) is gaining popularity as a research organism, largely due to its phenomenal regenerative capabilities [1]. Acomys has been shown to regenerate damage to the skin [2][3][4][5][6][7], ear pinna [8][9][10], skeletal muscle [11], kidney [12], heart [13][14][15], and spinal cord [16,17]. Remarkably, all these findings have occurred within the last decade.…”

Section: Introductionmentioning

confidence: 99%

Generation and characterization of two immortalized dermal fibroblast cell lines from the spiny mouse (Acomys)

Dill

Tabatabaei

Kamat

et al. 2022

Preprint

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The spiny mouse (Acomys) is gaining popularity as a research organism due to its phenomenal regenerative capabilities. Acomys recovers from injuries to several organs without fibrosis. For example, Acomys heals full thickness skin injuries with rapid re-epithelialization of the wound and regeneration of hair follicles, sebaceous glands, erector pili muscles, adipocytes, and dermis without scarring. Understanding mechanisms of Acomys regeneration may uncover potential therapeutics for wound healing in humans. However, access to Acomys colonies is limited and primary fibroblasts can only be maintained in culture for a limited time. To address these obstacles, we generated immortalized Acomys dermal fibroblast cell lines using two methods: transfection with the SV40 large T antigen and spontaneous immortalization. The two cell lines (AcoSV40 and AcoSI-1) maintained the morphological and functional characteristics of primary Acomys fibroblasts, including maintenance of key fibroblast markers and ECM deposition. The availability of these cells will lower the barrier to working with Acomys as a model research organism, increasing the pace at which new discoveries to promote regeneration in humans can be made.

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“…The fact some animals can regenerate complex parts of their body after injury has captured our imaginations for hundreds of years. For example, multiple fish species – including the goldfish, trout and zebrafish – are able to regenerate their kidneys throughout their lifetime ( Reimschuessel, 2001 ), and the spiny mouse also exhibits striking renal regenerative abilities ( Okamura et al, 2021 ). Humans, on the other hand, lack this power and stop producing nephrons – the functional units of the kidney – about 36 weeks after conception, and can only partially repair damaged nephrons ( Romagnani et al, 2013 ).…”

mentioning

confidence: 99%

Renal interstitial cells to the rescue

Wesselman

Wingert

2023

eLife

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Spiny mice activate unique transcriptional programs after severe kidney injury regenerating organ function without fibrosis

Cited by 27 publications

References 55 publications

GENOME REPORT: Chromosome-scale genome assembly of the African spiny mouse (Acomys cahirinus)

GENOME REPORT: Chromosome-scale genome assembly of the African spiny mouse (Acomys cahirinus)

Generation and characterization of two immortalized dermal fibroblast cell lines from the spiny mouse (Acomys)

Renal interstitial cells to the rescue

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