Spirulina cultivated under different light emitting diodes: Enhanced cell growth and phycocyanin production

Prates, Denise da Fontoura; Radmann, Elisângela Martha; Duarte, Jessica Hartwig; Morais, Michele Greque de; Costa, Jorge Alberto Vieira

doi:10.1016/j.biortech.2018.01.122

Cited by 101 publications

(34 citation statements)

References 37 publications

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“…Phycocyanin degradation by temperature (Antelo et al, 2008) and light (Jespersen et al, 2005) over time are potential causes of extraction efficiency loss that we considered minimal in our experiments because moss samples were always kept at cool temperature and in the dark during the extraction. Repeatability reported here are also similar to values reported in literature for pure culture (Kissoudi et al, 2018;Prates et al, 2018). Thus, our results show that the efficiency of cyanobacteria cell wall disruption is the principal factor that could affect the quality of phycocyanin measurements but that, overall, phycocyanin can be accurately and reliably quantified for large cyanobacterial cell density and moss mass ranges.…”

Section: Phycocyanin Extraction Methods Characterization and Validationsupporting

confidence: 90%

“…In this study, all samples (cyanobacteria cultures and mosses) had phycocyanin concentration above those limits. Phycocyanin apparent recoveries in moss found in this study are close to the average apparent recovery reported in pure cultures (50-60%, Tavanandi et al, 2018) but optimized methods can reach 90-92% (Kissoudi et al, 2018;Prates et al, 2018;Tavanandi et al, 2018). Phycocyanin concentration measured in cyanobacteria cultures depends greatly on the extraction process (e.g., solvent, extraction time, cell wall disruption technique; Abalde et al, 1998;Reis et al, 1998).…”

Section: Phycocyanin Extraction Methods Characterization and Validationsupporting

confidence: 81%

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Quantification of Moss-Associated Cyanobacteria Using Phycocyanin Pigment Extraction

2021

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In the boreal forest, cyanobacteria can establish associations with feather moss and realize the biological nitrogen fixation (BNF) reaction, consisting in the reduction of atmospheric dinitrogen into bioavailable ammonium. In this ecosystem, moss-associated cyanobacteria are the main contributors to BNF by contributing up to 50% of new N input. Current environmental changes driven by anthropogenic activities will likely affect cyanobacteria activity (i.e., BNF) and populations inhabiting mosses, leading to potential important consequences for the boreal forest. Several methods are available to efficiently measure BNF activity, but quantifying cyanobacteria biomass associated with moss is challenging because of the difficulty to separate bacteria colonies from the host plant. Attempts to separate cyanobacteria by shaking or sonicating in water were shown to be poorly efficient and repeatable. The techniques commonly used, microscopic counting and quantitative PCR (qPCR) are laborious and time-consuming. In aquatic and marine ecosystems, phycocyanin (PC), a photosynthesis pigment produced by cyanobacteria, is commonly used to monitor cyanobacteria biomass. In this study, we tested if PC extraction and quantification can be used to estimate cyanobacteria quantity inhabiting moss. We report that phycocyanin can be easily extracted from moss by freeze/thaw disturbance of cyanobacteria cells and can be quickly and efficiently measured by spectrofluorometry. We also report that phycocyanin extraction is efficient (high recovery), repeatable (relative SD < 13%) and that no significant matrix effects were observed. As for aquatic systems, the main limitation of cyanobacteria quantification using phycocyanin is the difference of cellular phycocyanin content between cyanobacteria strains, suggesting that quantification can be impacted by cyanobacteria community composition. Nonetheless, we conclude that phycocyanin extraction and quantification is an easy, rapid, and efficient tool to estimate moss-associated cyanobacteria number.

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Section: Phycocyanin Extraction Methods Characterization and Validationsupporting

confidence: 90%

Section: Phycocyanin Extraction Methods Characterization and Validationsupporting

confidence: 81%

Quantification of Moss-Associated Cyanobacteria Using Phycocyanin Pigment Extraction

2021

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“…The fatty acid ester found at the greatest percentage was C16:0 (40.60–45.75%), followed by C20:0, C18:2n6c and C18:1n9c. Prates et al [47] reported that palmitic acid was predominant in the fatty acid profile of S . platensis .…”

Section: Resultsmentioning

confidence: 99%

Mixotrophic cultivation of Spirulina platensis in dairy wastewater: Effects on the production of biomass, biochemical composition and antioxidant capacity

et al. 2019

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Mixotrophic cultivation of microalgae provides a very promising alternative for producing carbohydrate-rich biomass to convert into bioethanol and value-added biocompounds, such as vitamins, pigments, proteins, lipids and antioxidant compounds. Spirulina platensis may present high yields of biomass and carbohydrates when it is grown under mixotrophic conditions using cheese whey. However, there are no previous studies evaluating the influence of this culture system on the profile of fatty acids or antioxidant compounds of this species, which are extremely important for food and pharmaceutical applications and would add value to the cultivation process. S. platensis presented higher specific growth rates, biomass productivity and carbohydrate content under mixotrophic conditions; however, the antioxidant capacity and the protein and lipid content were lower than that of the autotrophic culture. The maximum biomass yield was 2.98 ±0.07 g/L in growth medium with 5.0% whey. The phenolic compound concentration was the same for the biomass obtained under autotrophic and mixotrophic conditions with 2.5% and 5.0% whey. The phenolic compound concentrations showed no significant differences except for that in the growth medium with 10.0% whey, which presented an average value of 22.37±0.14 mg gallic acid/g. Mixotrophic cultivation of S. platensis using whey can be considered a viable alternative to reduce the costs of producing S. platensis biomass and carbohydrates, shorten cultivation time and produce carbohydrates, as it does not require adding expensive chemical nutrients to the growth medium and also takes advantage of cheese whey, an adverse dairy industry byproduct.

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“…Lee, Erickson and Yang (1987), using urea as a source of nitrogen, observed that the greatest bioenergetic gains occurred in conditions of lower light intensity, while the opposite phenomenon occurred in conditions of higher light intensity. Other authors point to better results with the use of constant lighting in addition to the use of LED lamps, or in a set of fluorescent lamps (HO et al, 2018;PRATES et al, 2018). Despite providing a greater gain in growth, the continuous use of artificial lighting is expensive and may affect the economic viability of the activity (GOVINDJEE et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of phycocyanin production by marine microalgae Arthrospira platensis, grown in fish wastewater

Gomes

Andrade

Facundo

et al. 2020

BJD

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Phycocyanins are water-soluble proteins that work as accessory pigments and have several properties, such as immunostimulant action, cholesterol reducing effect, anti-inflammatory, antiviral, anticancer, antioxidant effects, among other uses. This study aimed to evaluate the accumulation of phycocyanin and biomass production of the microalgae Arthrospira (Spirulina) platensis grownin fish effluent. The experiment was carried out in two phases. The first developed in an indoor environment with controlled conditions, while the second, in a recirculation system, in an outdoor environment, using for both phases the Venkataraman media (control) and Nile tilapia effluent. There was no significant difference (p>0.05) between the performance of algae grown under the same conditions. Phycocyanin concentrations were higher for the treatments using modified Venkataraman medium. The purification process by ion exchange chromatography resulted in higher pigment concentrations for the eluted fractions with 0.2 M NaCl for all crude phycocyanin extracts.

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Spirulina cultivated under different light emitting diodes: Enhanced cell growth and phycocyanin production

Cited by 101 publications

References 37 publications

Quantification of Moss-Associated Cyanobacteria Using Phycocyanin Pigment Extraction

Quantification of Moss-Associated Cyanobacteria Using Phycocyanin Pigment Extraction

Mixotrophic cultivation of Spirulina platensis in dairy wastewater: Effects on the production of biomass, biochemical composition and antioxidant capacity

Evaluation of phycocyanin production by marine microalgae Arthrospira platensis, grown in fish wastewater

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