Since the technology of direct injected engines has become more and more prevalent, combustion anomalies such as pre-ignition events have been occurring with increasing frequency. The interaction between the fuel-spray and the oil wetted cylinder liner is considered to be a partial cause for these phenomena. The incoming fuel jet can lead to a detachment of secondary droplets, which may affect pre-ignition events due to their changed flammability properties. To characterize these secondary droplets, spatially and temporally highly resolved optical investigations on the fuel-oil-film-interaction are carried out in this work. Using laser induced fluorescence and two high-speed cameras both fluids are optically separated. Thereby the secondary droplets can be detected and characterized in size, velocity vector, and composition.