Splenectomy ameliorates acute multiple organ damage induced by liver warm ischemia reperfusion in rats

Jiang, Hongchi; Meng, F.; Wei, Li; Tong, Lei; Qiao, Haiquan; Sun, Xueying

doi:10.1016/j.surg.2006.03.024

Cited by 91 publications

(87 citation statements)

References 42 publications

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“…In particular, splenectomy was associated with worse hepatic IRI; however, other factors may contribute, including reduction in pressure in the portal vein, which might increase the arterial blood supply to the liver, promoting hepatic recovery after IRI. 10 Our results are consistent with studies demonstrating the potential role of the spleen in modulating the inflammatory response to organ injury. Given that the cholinergic anti-inflammatory pathway appears to be an intrinsic pathway that is initiated by the splenic nerve, we tested and confirmed that the deleterious effect of splenectomy could be recapitulated with splenic denervation alone.…”

Section: P=0004 (Iri Versus Us Plus Iri)supporting

confidence: 91%

“…Some work demonstrated a protective effect of prior splenectomy in animal models of organ injury. 10 In contrast, others demonstrated that prior splenectomy had deleterious effects. 8 Prophylactic treatments for AKI were ineffective in previously splenectomized animals, 9 including the ultrasound treatment described here.…”

Section: P=0004 (Iri Versus Us Plus Iri)mentioning

confidence: 99%

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Ultrasound Modulates the Splenic Neuroimmune Axis in Attenuating AKI

Gigliotti

Huang

Bajwa

et al. 2015

Journal of the American Society of Nephrology

122

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We showed previously that prior exposure to a modified ultrasound regimen prevents kidney ischemiareperfusion injury (IRI) likely via the splenic cholinergic anti-inflammatory pathway (CAP) and a7 nicotinic acetylcholine receptors (a7nAChR). However, it is unclear how ultrasound stimulates the splenic CAP. Further investigating the role of the spleen in ischemic injury, we found that prior splenectomy (-7d) or chemical sympathectomy of the spleen with 6-hydroxydopamine (6OHDA; -14d) exacerbated injury after subthreshold (24-minute ischemia) IRI. 6-OHDA-induced splenic denervation also prevented ultrasoundinduced protection of kidneys from moderate (26-minute ischemia) IRI. Ultrasound-induced protection required hematopoietic but not parenchymal a7nAChRs, as shown by experiments in bone marrow chimeras generated with wild-type and a7nAChR -/-mice. Ultrasound protection was associated with reduced expression of circulating and kidney-derived cytokines. However, splenocytes isolated from mice 24 hours after ultrasound treatment released more IL-6 ex vivo in response to LPS than splenocytes from sham mice. Adoptive transfer of splenocytes from ultrasound-treated (but not sham) mice to naïve mice was sufficient to protect kidneys of recipient mice from IRI. Ultrasound treatment 24 hours before cecal ligation puncture-induced sepsis was effective in reducing plasma creatinine in this model of AKI. Thus, splenocytes of ultrasound-treated mice are capable of modulating IRI in vivo, supporting our ongoing hypothesis that a modified ultrasound regimen has therapeutic potential for AKI and other inflammatory conditions.

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Section: P=0004 (Iri Versus Us Plus Iri)supporting

confidence: 91%

Section: P=0004 (Iri Versus Us Plus Iri)mentioning

confidence: 99%

Ultrasound Modulates the Splenic Neuroimmune Axis in Attenuating AKI

Gigliotti

Huang

Bajwa

et al. 2015

Journal of the American Society of Nephrology

122

View full text Add to dashboard Cite

show abstract

“…Moreover, the fact that a large amount of splenic tissue connects to liver tissue through the portal vein system, suggests the existence of a humoral factor originating in the spleen, which thus inhibits hepatic regeneration and promotes liver fibrosis. In rats submitted do hepatic ischemia/reperfusion, prior splenectomy ameliorated acute multiple organ damage (Jiang et al, 2007). These findings, associated to the favorable serum activity of ALT, AST and LDH in our splenectomized rats, partially explain the high hepatic biodistribution of 99mTc-phytate in the operated animals.…”

Section: Discussionsupporting

confidence: 50%

Biodistribution of the radiopharmaceutical technetium-99m-sodium phytate in rats after splenectomy

Pereira

Açucena

Neto

et al. 2008

Braz. arch. biol. technol.

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“…Myeloperoxidase (MPO) activity in liver tissue was determined by a method described in a report by our group (16).…”

Section: Myeloperoxidase Activitymentioning

confidence: 99%

Glycyrrhizin attenuates endotoxin- induced acute liver injury after partial hepatectomy in rats

Tang

Qiao

Meng

et al. 2007

Braz J Med Biol Res

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Massive hepatectomy associated with infection induces liver dysfunction, or even multiple organ failure and death. Glycyrrhizin has been shown to exhibit anti-oxidant and anti-inflammatory activities. The aim of the present study was to investigate whether glycyrrhizin could attenuate endotoxin-induced acute liver injury after partial hepatectomy. Male Wistar rats (6 to 8 weeks old, weighing 200-250 g) were randomly assigned to three groups of 24 rats each: sham, saline and glycyrrhizin. Rats were injected intravenously with lipopolysaccharide (LPS) 24 h after 70% hepatectomy. Glycyrrhizin, pre-administered three times with 24 h intervals 48 h before hepatectomy, prolonged the survival of rats submitted to partial hepatectomy and LPS injection, compared with saline controls. Glycyrrhizin was shown to attenuate histological hepatic changes and significantly reduced serum levels of aspartate aminotransferase, alanine aminotransferase, and lactic dehydrogenase, at all the indicated times (6 rats from each were sacrificed 1, 3, 6, and 9 h after LPS injection), compared with saline controls. Glycyrrhizin also significantly inhibited hepatocyte apoptosis by down-regulating the expression of caspase-3 and inhibiting the release of cytochrome C from mitochondria into the cytoplasm. The anti-inflammatory activity of glycyrrhizin may rely on the inhibition of release of tumor necrosis factor-α, myeloperoxidase activity, and translocation of nuclear factor-kappa B into the nuclei. Glycyrrhizin also up-regulated the expression of proliferating cell nuclear antigen, implying that it might be able to promote regeneration of livers harmed by LPS. In summary, glycyrrhizin may represent a potent drug protecting the liver against endotoxin-induced injury, especially after massive hepatectomy.

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Splenectomy ameliorates acute multiple organ damage induced by liver warm ischemia reperfusion in rats

Cited by 91 publications

References 42 publications

Ultrasound Modulates the Splenic Neuroimmune Axis in Attenuating AKI

Ultrasound Modulates the Splenic Neuroimmune Axis in Attenuating AKI

Biodistribution of the radiopharmaceutical technetium-99m-sodium phytate in rats after splenectomy

Glycyrrhizin attenuates endotoxin- induced acute liver injury after partial hepatectomy in rats

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