Splenocyte Cytokine Profile in Mouse with Oral Mucosa-sensitization and Oral-tolerization by NiSO4

Abstract

Metals used in the oral cavity have been reported to cause various allergic diseases of the skin and mucosa. Skin manifestations due to dental restorations appear not only in the oral cavity, but also on the hands, feet or the whole body, as in the cases of pustulosis palmoplantaris and lichen planus. These phenomena implicate different pathogeneses from that of conventional skin sensitization and tolerance. Therefore, we compared skin and oral mucosa sensitization with nickel and oral tolerance for nickel in a mouse model. Female C57BL/6J mice were sensitized by injection of NiSO 4 into the skin or oral mucosa. Allergic reactions were evaluated by the mouse ear swelling test and splenocyte proliferation and cytokine profiles. Skin and oral mucosa sensitization succeeded in all mice. Ear swelling was significantly greater in the skin-than in the oral mucosa-sensitized mice at 48 hr after challenge. Ear swelling was also suppressed by single oral administration of NiSO 4 in both the skin-and oral mucosa-sensitized mice to the level of that in nonsensitized mice. Splenocytes from skin-sensitized mice proliferated similarly to those from oral mucosa-sensitized mice. Splenocytes from orally-tolerized mice also showed similar proliferation activity to those from skin and oral mucosa-sensitized mice. In the challenge phase, IL-2, IFN-␥, and IL-10 production was induced in splenocytes from both skin-and oral mucosa-sensitized mice. However, IL-4 was induced only in those from skin-sensitized mice. In addition, IL-4 in splenocytes from oral mucosa-sensitized mice was up-regulated to the level in those from skin-sensitized mice by oral tolerance. These results suggest that sensitization sites in mice influence not only the degree of excitation, but also Th-1 and Th-2 balance in the challenge phase and oral tolerance.

The β-sitosterol attenuates atopic dermatitis-like skin lesions through down-regulation of TSLP

“…Cytokines produced from the spleen mediate various allergic diseases, such as AD, asthma, and ear swelling. 47–49 Moreover, mixed Th1 and Th2 type cytokines were produced from splenocytes of skin-sensitized mice. 49 The release of TNF-α is of major importance in the initiation and development of many inflammatory skin disorders.…”

“…47–49 Moreover, mixed Th1 and Th2 type cytokines were produced from splenocytes of skin-sensitized mice. 49 The release of TNF-α is of major importance in the initiation and development of many inflammatory skin disorders. 50 Histamine did not affect the phosphorylation of ERK1/2, which is involved in allergic inflammation via H1 receptors in splenocytes from TNF-α knockout mice.…”