In this paper, we generalize the pinching operation on two edges of graphs to binary matroids and investigate some of its basic properties. For n ≥ 2, the matroid that is obtained from an n-connected matroid by this operation is a k-connected matroid with k ∈ {2, 3, 4} or is a disconnected matroid. We find conditions to guarantee this k. Moreover, we show that Eulerian binary matroids are characterized by this operation and we also provide some interesting applications of this operation.