SPME‐GC/MS Analysis of Volatile Compounds Contained in the Insect Larvae of <i>Tenebrio molitor</i> and <i>Leptinotarsa decemlineata</i> before and after Using Insecticides

Wojciechowska, Marta; Gołębiowski, Marek

doi:10.1002/cbdv.201900743

Cited by 7 publications

(4 citation statements)

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“…Differences were also visible depending on the insecticide used, the time elapsed since its application and the sex of the insects. Similar conclusions and results have been described for the larvae of T. molitor and L. decemlineata after using insecticides containing cyfluthrin and deltamethrin (Wojciechowska and Gołębiowski 2020). Using the Folch method, changes in the composition of internal compounds contained in the fat body after applying insecticides have been described in the larvae, females and males of T. molitor (Wojciechowska et al, 2019).…”

Section: Discussionsupporting

confidence: 71%

Composition of volatile compounds in male and female Tenebrio molitor and Leptinotarsa decemlineata before and after the application of insecticides

2022

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Some insects can be used as food, while others can affect the destruction of crops and the reduction of food reserves. The studies described in this article showed quantitative and qualitative changes in the composition of volatile compounds contained in adult Tenebrio molitor and Leptinotarsa decemlineata insects after applying insecticides. The samples were prepared by SPME and the final determinations were carried out by GC/MS. The volatile compounds that were present only in the insects after the application of insecticides (or in a larger quantities) could be due to the insecticides, as an insect defense response to these insecticides. For example, in adult T. molitor insects, the percentage of alkanes ranged from 38.63 to 59.05% (male), and from 38.89% to 60.20 (female), depending on the insecticide used and the time elapsed since its application. In contrast, in L. decemlineata, the percentage of alkanes ranged from 43.84 to 61.85% (female), and from 42.41 to 60.11% (male). The results of the present study can be useful in understanding the i defense strategies of insects against insecticides.

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Section: Discussionsupporting

confidence: 71%

Composition of volatile compounds in male and female Tenebrio molitor and Leptinotarsa decemlineata before and after the application of insecticides

2022

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“…Solid-phase microextraction (SPME): To extract volatile compounds from the insect (Table 1), every sample was weighed before the extraction method (direct SPME or micro-QuEChERS). The extraction of volatile compounds from the free space of the samples was performed using polydimethylsiloxane/carboxen/divinylbenzene (PDMS/CAR/DVB) at a temperature of 105 • C. The extraction time for the fiber was 40 min, based on reported work related to pesticide detection in larvae [14].…”

Section: Sample Distribution For Analysismentioning

confidence: 99%

“…In response to this issue, several strategies have been explored to monitor its presence in the environment, regulate its use, and explore alternative options. It is important to note, however, that these initiatives are still in their early stages or may prove to be economically burdensome, particularly in countries with moderate-to-low incomes [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…The solid-phase microextraction (SPME) technique, combined with mass spectrometry (GC/MS), has been employed for the analysis of volatile components in Tenebrio molitor (Coleoptera: Tenebrionidae) and Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) larvae. These studies have revealed the presence of aldehydes, alkaloids, esters, ketones, aromatic ketones, fatty acids, and terpenes which were detected 24 and 48 h after the application of the insecticides fluthrin, thiamethoxam, and acetamiprid [14].…”

Section: Introductionmentioning

confidence: 99%

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What Can Insects Tell Us about the Use of Pesticides? The Detection and Quantification of Chlorpyrifos via QuEChERS and HEADSPACE-SPME Methods Using GC/MS/MS

Valdés,

Silva-Neto,

Salas

et al. 2023

Agronomy

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The global use of pesticides, exceeding 4 million tons annually, poses substantial threats to public health and the environment. Numerous studies emphasize the correlation between pesticide exposure, genotoxicity, and neurobehavioral effects, which particularly impact children and agricultural workers. Recent research underscores the health risks associated with moderately toxic pesticides like chlorpyrifos. Chlorpyrifos contamination in crops, a result of extensive pesticide use since the 1960s, raises significant concerns regarding human health and the environment. Given its potential atmospheric volatilization from crops, alternative detection methods are imperative. This study employs gas chromatography coupled with mass spectrometry (GC/MS) in MS/MS mode, focusing on detecting chlorpyrifos in Chilean lettuce, using insects as indicators. Two extraction methods, micro-QuEChERS and HEADSPACE-SPME, were compared, with HEADSPACE-SPME demonstrating superior sensitivity (6.77 ng/mg per sample vs. 3.99 ng/mg per sample) and offering time- and solvent-saving advantages. Additionally, HEADSPACE-SPME preserves samples for future research, enhancing its utility. The study confirms the presence of pesticide residues in insects from lettuce crops, with 52.3% displaying chlorpyrifos when the SPME method was used. Predatory and parasitoid insect families like Braconidae and Ichneumonidae show high pesticide loads. The findings of this study emphasize the widespread contamination of chlorpyrifos in lettuce crop insects and highlight the efficacy of SPME for detection, suggesting its broader applicability for evaluating pesticide residues in horticultural systems. This optimized method holds promise for advancing chlorpyrifos contamination detection in agroecosystems, contributing to environmental monitoring and food security.

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Unlocking the Secrets of Insects: The Role of Mass Spectrometry to Understand the Life of Insects

Mayorga‐Martino,

Mansurova,

Calla‐Quispe

et al. 2024

Mass Spectrometry Reviews

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Chemical signaling is crucial during the insect lifespan, significantly affecting their survival, reproduction, and ecological interactions. Unfortunately, most chemical signals insects use are impossible for humans to perceive directly. Hence, mass spectrometry has become a vital tool by offering vital insight into the underlying chemical and biochemical processes in various variety of insect activities, such as communication, mate recognition, mating behavior, and adaptation (defense/attack mechanisms), among others. Here, we review different mass spectrometry‐based strategies used to gain a deeper understanding of the chemicals involved in shaping the complex behaviors among insects and mass spectrometry‐based research in insects that have direct impact in global economic activities.

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SPME‐GC/MS Analysis of Volatile Compounds Contained in the Insect Larvae of Tenebrio molitor and Leptinotarsa decemlineata before and after Using Insecticides

Cited by 7 publications

References 36 publications

Composition of volatile compounds in male and female Tenebrio molitor and Leptinotarsa decemlineata before and after the application of insecticides

Composition of volatile compounds in male and female Tenebrio molitor and Leptinotarsa decemlineata before and after the application of insecticides

What Can Insects Tell Us about the Use of Pesticides? The Detection and Quantification of Chlorpyrifos via QuEChERS and HEADSPACE-SPME Methods Using GC/MS/MS

Unlocking the Secrets of Insects: The Role of Mass Spectrometry to Understand the Life of Insects

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