SPME–HPLC analysis of Allium lacrymatory factor and thiosulfinates

Jaillais, B; Cadoux, F; Auger, J.

doi:10.1016/s0039-9140(99)00124-1

Cited by 15 publications

(12 citation statements)

References 6 publications

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“…Conversely, leeks that have been attacked by leek moth or mechanically wounded release volatiles, including numerous thiosulfinates and zwiebelanes, compounds previously identified in analytical conditions (Auger et al, 1989;Jaillais et al, 1999). The lack of qualitative difference between volatiles released after herbivore damage and mechanical wounding has also been reported in Solanaceae such as tomato and potato (Bolter et al, 1997) and in crucifers (Mattiacci et al, 1994).…”

Section: Discussionmentioning

confidence: 91%

“…Through a Teflon membrane placed over the other side opening, a polyacrylate solid phase microextraction (SPME) fiber (85-mm diam; Supelco, Bellefonte, PA, USA) was introduced and used to trap odors released in the headspace for 15 min. The second step used an electric crusher to grind the preceding leeks to compensate for natural degradation of some sulfur volatiles on the SPME fiber (Jaillais et al, 1999;Arnault et al, 2000). The resulting liquid was filtered through a blutter cloth.…”

Section: Methodsmentioning

confidence: 99%

“…These amino acids are considered precursors of sulfur volatiles because their contact with the enzyme alliinase produced after the rupture of plant tissue cells leads to the formation of sulfur volatiles mainly in the form of thiosulfinates that subsequently break down and rearrange into disulfides and trisulfides (Auger et al, 1989;Jaillais et al, 1999). This complex involving alk(en)yl-cystein sulfoxidesYalliinaseY thiosulfinates and di-or trisulfides is probably involved in the plant's defense strategy.…”

Section: Introductionmentioning

confidence: 99%

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Increased Sulfur Precursors and Volatiles Production by the Leek Allium porrum in Response to Specialist Insect Attack

et al. 2005

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Abstract-To defend themselves against herbivory, plants use a variety of direct and indirect strategies involving induced increases in secondary substances. Species of the Allium genus (Alliaceae), such as the leek Allium porrum (L.), produce nonprotein sulfur amino acids derived from cysteine, i.e., alk(en)yl-cysteine sulfoxides that are precursors of volatile thiosulfinates and disulfides. These defend most species including the specialist leek moth, Acrolepiopsis assectella. We determined by measuring the increase in the sulfur precursor propyl-cysteine sulfoxide (PCSO) if production of this precursor is induced in response to moth attack and mechanical wounding. The concentration of PCSO was determined by HPLC in 2-or 6-mo-old leeks after attacks of various intensity either by the specialist leek moth or by a generalist moth, Agrotis ipsilon. Injury-induced release of sulfur volatiles was measured by GC/MS after the attacks. Results showed an increase in the production of sulfur compounds in both the precursor and volatile form, occurring only in association with intensive attacks by leek moths. The increase in sulfur precursors also led to an increase in the release of sulfur volatiles. This induced response may provide an effective defense strategy against the plant's main natural enemy, both directly and indirectly by attracting entomophagous insects.

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Section: Discussionmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Increased Sulfur Precursors and Volatiles Production by the Leek Allium porrum in Response to Specialist Insect Attack

et al. 2005

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“…Celles-ci sont émises lors de la destruction des cellules. Les acides aminés précurseurs sont alors mis en présence d'une enzyme, l'al- (Messiaen et al, 1993 ;Moreau et al, 1996 ;Jaillais et al, 1999 ;Bakrii & Douglas, 2005). Les espèces du genre Allium ne libèrent leur odeur caractéris-tique que lorsque les tissus sont coupés ou écrasés.…”

Section: A Identification Des Précurseurs Des Substances Soufrées Vounclassified

Différences et similitudes des métabolites secondaires chez deux espèces du genreAllium, Allium roseumL. etAllium ampeloprasumL

Najjaa¹,

Zouari²,

Arnault

et al. 2011

Acta Botanica Gallica

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Différences et similitudes des métabolites secondaires chez deux espèces du genre Allium, Allium roseum L. et Allium ampeloprasum L.Résumé.-Ce travail a porté sur deux Allium spontanés prisés en raison de leur valeur alimentaire et leurs vertus thérapeutiques, A. roseum var. odoratissimum et A. ampeloprasum. Nous avons engagé un screening phytochimique de méta-bolites secondaires ainsi qu'une analyse qualitative de composés soufrés pré-curseurs des arômes d'Allium, les S-alk(en)yl cystéine sulfoxides. Les résultats montrent que ces espèces sont caractérisées par la présence de méthiine, alliine et isoalliine chez A. roseum, isoalliine et méthiine chez A. ampeloprasum. Ainsi A. roseum présente les caractéristiques aromatiques d'un ail spontané et A. ampeloprasum est plus proche d'un oignon. Les tests de caractérisation réa-lisés sur ces différents produits ont permis de mettre en évidence leur richesse en Abstract.-This study deals with the valorisation of medicinal and aromaticAllium to find new bioactive natural products. The phytochemical analyses of A. roseum and A. ampeloprasum powders and their aqueous and organic extracts are achieved. The results show that these species are characterized by the presence, among their components, of three precursors of organosulphurous volatile compounds. They are methiin, isoalliine and alliine in A. roseum and isoalliin and methiin in A. ampeloprasum. These differences in their flavours can correlated with the vicinity of A. roseum with garlic and A. ampeloprasum with onion. Phytochemical screening of Allium roseum shows the presence of active agents such as saponins, tannins, flavonoids, coumarins, steroids, cardiac glycosides, free quinone and iridoids.

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“…Jaillais et al [55] compared results obtained by headspace SPME with those obtained by liquid nitrogen trapping then classic injection, as sample pretreatment for LC-UV determination of Allium volatiles. The authors concluded that the analytical performance was improved by use of SPME, although some analytes were partially degraded during the desorption step.…”

Section: Biological Samplesmentioning

confidence: 99%

Coupling solid-phase microextraction to liquid chromatography. A review

Zambonin

2003

Anal Bioanal Chem

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Solid-phase microextraction (SPME) is a technique for extraction of organic compounds from gaseous, aqueous, and solid matrices. SPME is rapid and simple, ideal for automation and for in situ measurements, and no harmful solvents are needed. The principle of SPME involves equilibration of the analytes between the sample matrix and an organic polymeric phase coated on a fused-silica fiber. SPME is traditionally combined with analysis by gas chromatography (GC) and this combination has proved sensitive, accurate, and precise for quantitative analysis of different classes of volatile compound. More recently SPME has been coupled with liquid chromatography to widen its range of application to non-volatile and thermally unstable compounds also. This article reviews the status of SPME coupled with liquid chromatography. It focuses on different applications of the technique, e.g. environmental samples, biological fluids, and food samples, to show that SPME-HPLC has great potential in the analysis of a wide range of compounds in different matrices.

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SPME–HPLC analysis of Allium lacrymatory factor and thiosulfinates

Cited by 15 publications

References 6 publications

Increased Sulfur Precursors and Volatiles Production by the Leek Allium porrum in Response to Specialist Insect Attack

Increased Sulfur Precursors and Volatiles Production by the Leek Allium porrum in Response to Specialist Insect Attack

Différences et similitudes des métabolites secondaires chez deux espèces du genreAllium, Allium roseumL. etAllium ampeloprasumL

Coupling solid-phase microextraction to liquid chromatography. A review

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