Spodoptera exigua Nuclear Polyhedrosis Virus as a Candidate Viral Insecticide For the Beet Armyworm (Lepidoptera: Noctuidae)

Kolodny-Hirsch, Douglas M.; Warkentin, D.; Alvarado-Rodriguez, Benito; Kirkland, Reed L.

doi:10.1093/jee/86.2.314

Cited by 27 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the NPV isolated from S. exigua (SeMNPV) has been demonstrated to be more pathogenic and to have shorter speed of kill than any other NPV that is infectious to S. exigua . Several SeMNPV strains have been isolated from larvae collected in Egypt and The Netherlands (V LAK et al., 1981), California (G ELERNTER and F EDERICI , 1986), Spain (C ABALLERO et al., 1992b), Japan and Thailand (H ARA et al., 1995; K ONDO et al., 1994), and Florida (K OLODNY ‐H IRSCH et al., 1993). All of them have been characterized biochemically by restriction endonuclease analyses (REN) of viral DNA, showing that they can be distinguished from each other by one or more DNA restriction enzyme fragments.…”

Section: Introductionmentioning

confidence: 99%

Biochemical characterization of three nucleopolyhedrovirus isolates of Spodoptera exigua and Mamestra brassicae

Murillo

Muñóz

Lipa

et al. 2001

J Applied Entomology

View full text Add to dashboard Cite

Three nucleopolyhedroviruses (NPVs), of different geographical origins, were biochemically characterized and compared. Two that were isolated from Spodoptera exigua (Se‐UZB and Se‐SP3) in Uzbekistan and Spain were SeMNPV type, and the third which was isolated from Mamestra brassicae (Mb‐PL) in Poland was MbMNPV type. The Spanish isolate Se‐SP3 showed restriction endonuclease (REN) profiles that were closely related to two previously described Spanish strains Se‐SP1 and Se‐SP2, but had some unique and characteristic REN fragments. On the other hand, comparison between the Uzbekian (Se‐UZB) and the Spanish (Se‐SP1, Se‐SP2, and Se‐SP3) isolates from S. exigua showed unrelated REN profiles. However, the PstI and BglII profiles of Se‐UZB and Mb‐PL were identical, and very similar to the REN profiles of the MbMNPV strain, which constitutes the active component of Mamestrin® (NPP, Nagueres, France), a commercial bioinsecticide. It is therefore very likely that the Se‐UZB samples were cross‐infected by the Polish strain (Mb‐PL). This work presents two new strains of SeMNPV and MbMNPV, called Se‐SP3 and Mb‐PL, respectively, which were surveyed in two distant areas.

show abstract

Section: Introductionmentioning

confidence: 99%

Biochemical characterization of three nucleopolyhedrovirus isolates of Spodoptera exigua and Mamestra brassicae

Murillo

Muñóz

Lipa

et al. 2001

J Applied Entomology

View full text Add to dashboard Cite

show abstract

“…THE BEET ARMYWORM, Spodoptera exigua (Hü bner), is polyphagous and feeds on many economically important crops such as cabbage, celery, lettuce, cotton, and chrysanthemum (Smith 1989, Kolodny-Hirsch et al 1993, Diawara et al 1994, East et al 1994. In both the United States and the Netherlands, S. exigua is a serious pest of greenhouse-grown crops, and a nuclear polyhedrosis virus (SeMNPV) has been used to control beet armyworm populations (Vlak et al 1982, Smits et al 1987).…”

mentioning

confidence: 99%

Relative Effectiveness of Selected Stilbene Optical Brighteners as Enhancers of the Beet Armyworm (Lepidoptera: Noctuidae) Nuclear Polyhedrosis Virus

Shapiro

Argauer

2001

View full text Add to dashboard Cite

The addition of a stilbene optical brightener, Tinopal LPW, at 1% concentration (wt:wt) significantly reduced the LC50 of the beet armyworm nuclear polyhedrosis virus (SeMNPV) from 2.9 PIB/mm2 to 0.02 PIB/mm2. Moreover, the LT50 of SeMNPV was reduced by 34% by the addition of Tinopal LPW. Seven other structurally related stilbene brighteners were also tested as viral enhancers. Five of these brighteners (Tinopal LPW, Blankophor BBH, Blankophor HRS, Blankophor P167, and Blankophor RKH) reduced LD50, whereas three brighteners (Blankophor BSU, Blankophor DML, and Blankophor LPG) had little effect. Among the active brighteners, LC50s were reduced by 10.5-fold (Blankophor P167), 52.4-fold (Blankophor RKH), 87.3-fold Tinopal LPW), 131-fold (Blankophor BBH), and >400-fold (Blankophor HRS). LT50s were also decreased by the addition of Blankophor BBH, Blankophor P167, and Blankophor RKH, but were increased by the addition of Blankophor BSU, Blankophor DMLO, and Blankophor LPG to SeMNPV suspensions.

show abstract

“…The virus has been used in laboratory and Þeld studies (Chauthani and Rehnborg 1971, Gelernter et al 1986, Smits and Vlak 1988, Kolodny-Hirsch et al 1993). In 1995 this virus was registered in the United States SPOD-X for use on 37 crops against beet armyworm populations.…”

mentioning

confidence: 99%

Enhancement in Activity of Homologous and Heterologous Baculoviruses Infectious to Beet Armyworm (Lepidoptera: Noctuidae) by an Optical Brightener

Shapiro

2000

View full text Add to dashboard Cite

The nuclear polyhedrosis virus (NPV) from the beet armyworm, Spodoptera exigua (Hübner) (SeMNPV), was the most active virus tested against the beet armyworm (LC50 = 4.1 PIBs/mm2), followed by nuclear polyhedrosis viruses from the alfalfa looper, Autographa californica (Speyer) (AcMNPV; LC50 = 92.6 PIBs/mm2), and the celery looper, Anagrapha falcifera (Kirby) (AfMNPV; LC50 = 195.7 PIBs/mm2). In the case of the nuclear polyhedrosis virus from the bollworm, Helicoverpa armigera (Hübner), LC50s could only be obtained for five/six replicates, whereas LC50s could only be obtained for two/six replicates for the nuclear polyhedrosis virus from the wax moth, Galleria mellonella (L.) (GmMNPV). When an optical brightener Tinopal LPW was added to virus suspensions, LC50 values were reduced by 130-fold for both SeMNPV and AcMNPV and by 300-fold for AfMNPV. The addition of Tinopal LPW greatly increased the activities of HaMNPV and GmMNPV. In terms of speed of kill, Tinopal LPW reduced the LT50s for all nuclear polyhedrosis viruses by 30-40%.

show abstract

Spodoptera exigua Nuclear Polyhedrosis Virus as a Candidate Viral Insecticide For the Beet Armyworm (Lepidoptera: Noctuidae)

Cited by 27 publications

References 0 publications

Biochemical characterization of three nucleopolyhedrovirus isolates of Spodoptera exigua and Mamestra brassicae

Biochemical characterization of three nucleopolyhedrovirus isolates of Spodoptera exigua and Mamestra brassicae

Relative Effectiveness of Selected Stilbene Optical Brighteners as Enhancers of the Beet Armyworm (Lepidoptera: Noctuidae) Nuclear Polyhedrosis Virus

Enhancement in Activity of Homologous and Heterologous Baculoviruses Infectious to Beet Armyworm (Lepidoptera: Noctuidae) by an Optical Brightener

Contact Info

Product

Resources

About