Spodoptera frugiperda multiple nucleopolyhedrovirus as a potential biological insecticide: Genetic and phenotypic comparison of field isolates from Colombia

Barrera, Gloria; Simón, Oihane; Villamizar, Laura; Williams, Trevor; Caballero, Primitivo

doi:10.1016/j.biocontrol.2011.04.009

Cited by 68 publications

(81 citation statements)

References 49 publications

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“…These results are in line with data from previous studies performed with different variants of HearSNPV, in which variants of similar pathogenicities presented marked differences in speed of kill (36,50). Generally, the fastest-killing variants tend to produce lower numbers of OBs in each infected insect, because insects die faster and feed and grow less during the incubation period of infection, and the virus has less time to replicate (50)(51)(52)(53). However, we observed that HearLB1 was highly productive, despite being one of the fastestkilling variants.…”

Section: Discussionsupporting

confidence: 90%

A Novel Binary Mixture of Helicoverpa armigera Single Nucleopolyhedrovirus Genotypic Variants Has Improved Insecticidal Characteristics for Control of Cotton Bollworms

Arrizubieta

Simón

Williams

et al. 2015

Appl Environ Microbiol

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The genotypic diversity of two Spanish isolates of Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) was evaluated with the aim of identifying mixtures of genotypes with improved insecticidal characteristics for control of the cotton bollworm. Two genotypic variants, HearSP1A and HearSP1B, were cloned in vitro from the most pathogenic wild-type isolate of the Iberian Peninsula, HearSNPV-SP1 (HearSP1-wt). Similarly, six genotypic variants (HearLB1 to -6) were obtained by endpoint dilution from larvae collected from cotton crops in southern Spain that died from virus disease during laboratory rearing. Variants differed significantly in their insecticidal properties, pathogenicity, speed of kill, and occlusion body (OB) production (OBs/larva). HearSP1B was ϳ3-fold more pathogenic than HearSP1-wt and the other variants. HearLB1, HearLB2, HeaLB5, and HearLB6 were the fastest-killing variants. Moreover, although highly virulent, HearLB1, HearLB4, and HearLB5 produced more OBs/ larva than did the other variants. The co-occluded HearSP1B:LB6 mixture at a 1:1 proportion was 1.7-to 2.8-fold more pathogenic than any single variant and other mixtures tested and also killed larvae as fast as the most virulent genotypes. Serial passage resulted in modified proportions of the component variants of the HearSP1B:LB6 co-occluded mixture, suggesting that transmissibility could be further improved by this process. We conclude that the improved insecticidal phenotype of the HearSP1B:LB6 co-occluded mixture underlines the utility of the genotypic variant dissection and reassociation approach for the development of effective virus-based insecticides.A lphabaculoviruses (Baculoviridae) are lepidopteran-specific nucleopolyhedroviruses (NPVs) that have been used successfully as biological control agents against several agricultural and forest pests (1). These viruses are characterized by high intraspecific heterogeneity not only between isolates from different geographic regions (2, 3) but also within single isolates that often comprise mixtures of several genotypes present in different proportions (4-8). The molecular heterogeneity of genotypes is often associated with phenotypic differences in pathogenicity, speed of kill, and virus production (8, 9), which are traits of practical importance for the use of these viruses as biological insecticides (10). Infections involving mixtures of genotypes can result in positive (11-14) or negative (15-18) effects on a number of these traits.As natural alphabaculovirus populations in virus-killed insects comprise mixtures of genotypes, the genotypic interactions that modulate the insecticidal characteristics have attracted the attention of insect pathologists (8,(19)(20)(21) and have been used to develop novel products based on unique user-defined genotypic combinations in specific user-defined proportions that result in improved insecticidal properties of the final mixture (22,23). This has resulted in the generation of a novel paradigm for the development of baculovirus-based insectici...

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Section: Discussionsupporting

confidence: 90%

A Novel Binary Mixture of Helicoverpa armigera Single Nucleopolyhedrovirus Genotypic Variants Has Improved Insecticidal Characteristics for Control of Cotton Bollworms

Arrizubieta

Simón

Williams

et al. 2015

Appl Environ Microbiol

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“…However, our results show that SpltNPV-Pak-BNG kills S. litura larvae considerably faster than SpltNPV-G1. Previous studies demonstrate that the variation in biological activity of nucleopolyhedroviruses may depend on the insect colonies (Barrera, Simon, Villamizar, Williams, & Caballero, 2011;Erlandson, 2009). Our local S. litura biotype from the NARC colony expresses greater sensitivity to SpltNPV-Pak-BNG than to the exotic isolate SpltNPV-G1 and may be useful to study the biological properties of SpltNPVs.…”

Section: Discussionmentioning

confidence: 78%

Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

Ali¹,

Werf

Vlak

2017

Biocontrol Science and Technology

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Spodoptera litura is an emerging insect pest in a wide range of crops worldwide. The insect is difficult to control because of resistance development to synthetic insecticides and emerging resistance to Bacillus thuringiensis toxins. Therefore, there is a need to develop biological control agents, preferably from an indigenous source to avoid risks associated with the importation of exotic natural antagonists. A Pakistani isolate of S. litura nucleopolyhedrovirus (SpltNPV, Baculoviridae), SpltNPV-Pak-BNG, was obtained from the field and characterized biologically and genetically, and compared to a SpltNPV reference isolate, SpltNPV-G1, thought to be of Chinese origin. The dose-mortality response (LD 50 ) of SpltNPVPak-BNG was not significantly different from that of the reference isolate SpltNPV-G1, but the time-to-death (LT 50 ) was significantly shorter for SpltNPV-Pak-BNG than for SpltNPV-G1. DNA restriction enzyme profiling indicated that SpltNPV-Pak-BNG and SpltNPV-G1 are different viruses. Sequence analysis of 'ORF24', specific for SpltNPV (and S. littoralis NPV as ORF21), and the conserved baculovirus core genes polyhedrin, DNApol, pif-2 and lef-8 confirmed that this was indeed the case and that SpltNPV-Pak-BNG is a genuine SpltNPV variant, whereas the SpltNPV-G1 isolate we used is, in fact, a SpliNPV variant, renamed to SpliNPV-G1. The newly isolated SpltNPV-Pak-BNG has the potential for development as a biocontrol agent of S. litura in Pakistan.ARTICLE HISTORY

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“…The baculovirus isolated from the S. frugiperda has been studied as a potential biological insecticide for control of fall armyworms (Williams et al, 1999;Martínez et al, 2000;Barrera et al, 2011). The discovery of the 3AP2 isolate of SfMNPV with a faster speed of kill compared with the wild-type virus prompted these investigations to (1) confirm that the faster kill was maintained when applied to plants grown under glass house and field environments, (2) evaluate the production of the new isolate compared to a wild-type virus, and (3) evaluate the new isolate for potential impact on residual insecticidal activity.…”

Section: Discussionmentioning

confidence: 99%

Laboratory and field evaluations of the efficacy of a fast-killing baculovirus isolate from Spodoptera frugiperda

Behle

Popham

2012

Journal of Invertebrate Pathology

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Spodoptera frugiperda multiple nucleopolyhedrovirus as a potential biological insecticide: Genetic and phenotypic comparison of field isolates from Colombia

Cited by 68 publications

References 49 publications

A Novel Binary Mixture of Helicoverpa armigera Single Nucleopolyhedrovirus Genotypic Variants Has Improved Insecticidal Characteristics for Control of Cotton Bollworms

A Novel Binary Mixture of Helicoverpa armigera Single Nucleopolyhedrovirus Genotypic Variants Has Improved Insecticidal Characteristics for Control of Cotton Bollworms

Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

Laboratory and field evaluations of the efficacy of a fast-killing baculovirus isolate from Spodoptera frugiperda

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