Spoilage potential of<i>Vagococcus salmoninarum</i>in preservative-free, MAP-stored brown shrimp and differentiation from<i>Brochothrix thermosphacta</i>on streptomycin thallous acetate actidione agar

Calliauw, Fien; Horemans, Benjamin; Broekaert, Katrien; Michiels, Chris W.; Heyndrickx, Marc

doi:10.1111/jam.13107

Cited by 9 publications

(3 citation statements)

References 42 publications

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“…However, the volatile molecules produced by this species have low sensory impacts (Casaburi et al 2011). There is no available information of the newly detected storage organism Vagococcus vulneris, but the closely related V. salmoninarum is a verified producer of 2-heptanone, 2-nonanone, and 2-undecanone (Calliauw et al 2016). In the future, V. vulneris should be cultivated, identified with complete 16S rRNA sequencing, and its spoilage potential should be examined to clarify its effects on sensory characteristics.…”

Section: Discussionmentioning

confidence: 99%

Novel members of bacterial community during a short-term chilled storage of common carp (Cyprinus carpio)

et al. 2021

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This work aimed to identify the key members of the bacterial community growing on common carp (Cyprinus carpio) fillets during chilled storage with next-generation sequencing (NGS) and cultivation-dependent methods. Carp fillets were stored for 96 h at 2 °C and 6 °C with and without a vacuum package, and an additional frozen-thawed storage experiment was set for 120 days. Community profiles of the initial and stored fish samples were determined by amplicon sequencing. Conventional microbial methods were used parallelly for the enumeration and cultivation of the dominant members of the microbial community. Cultivated bacteria were identified with 16S rRNA sequencing and the MALDI-TOF MS method. Based on our results, the vacuum package greatly affected the diversity and composition of the forming microbial community, while temperature influenced the cell counts and consequently the microbiological criteria for shelf-life of the examined raw fish product. Next-generation sequencing revealed novel members of the chilled flesh microbiota such as Vagococcus vulneris or Rouxiella chamberiensis in the vacuum-packed samples. With traditional cultivation, 161 bacterial strains were isolated and identified at the species level, but the identified bacteria overlapped with only 45% of the dominant operational taxonomic units (OTUs) revealed by NGS. Next-generation sequencing is a promising and highly reliable tool recommended to reach a higher resolution of the forming microbial community of stored fish products. Knowledge of the initial microbial community of the flesh enables further optimization and development of processing and storage technology.

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Section: Discussionmentioning

confidence: 99%

Novel members of bacterial community during a short-term chilled storage of common carp (Cyprinus carpio)

et al. 2021

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“…thermosphacta as V . s almoninarum [42]. PNs007 T produced hydrogen sulphide when grown on iron agar, while STAA11 T and STAA25 did not.…”

Section: Phenotypic Characterizationmentioning

confidence: 99%

“…This allowed STAA11 and STAA25 to grow on STAA agar. Therefore, they could be misidentified in the same way as B. thermosphacta as V. salmoninarum [42]. PNs007 T produced hydrogen sulphide when grown on iron agar, while STAA11 T and STAA25 did not.…”

Section: Phenotypic Characterizationmentioning

confidence: 99%

Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged broiler meat

Johansson

Jääskeläinen

Säde

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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During our studies on spoilage microbiomes of modified atmosphere packaged broiler meat, we isolated three strains (PNs007T, STAA11T and STAA25) of unknown identity. In this present polyphasic taxonomy study, including genome-based analyses, we discovered that these isolates represent two novel species belonging to the genus Vagococcus . In all phylogenetic analyses, PNs007T was positioned very close to Vagococcus fessus but both the average nucleotide identity (ANI; 89.5 %) and digital DNA–DNA hybridization (dDDH; 38.3 %) values distinguished it as a novel vagococcal species. STAA11T and STAA25 were genetically highly similar (16S rRNA, ANI and dDDH 100 %). The phylogenetic position of STAA11T was adjacent to but out of the cluster containing V. fessus , Vagococcus coleopterorum and PNs007T. According to the ANI (76.2–76.4 %) and dDDH (<22.6 %) values it also represented a novel vagococcal species. Phenotypic characteristics and chemotaxonomic properties of both novel species were typical for vagococci and they contained C16 : 0 (25.5–30.1 %) and C18 : 1 ω9c (67.3–73.0 %) as the major cellular fatty acids. The streptomycin-resistant genotype of STAA11T and STAA25 allowing the growth on streptomycin thallous acetate actidione medium was considered to result from a modification in codon 104 of the rpsL gene leading to P104A substitution. The ability of STAA11T and STAA25 to produce ammonia from arginine separated them from PNs007T, which did not show arginine deiminase activity. We propose the names Vagococcus proximus sp. nov. (type strain PNs007T=DSM 115185T=CCUG 76696T) and Vagococcus intermedius sp. nov. (type strain STAA11T=DSM 115183T=CCUG 76697T) for these novel species.

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Comparative analysis of gut bacterial community composition during a single day cycle in Chinese mitten crab (Eriocheir sinensis)

Yu¹,

Li²,

Jin³

et al. 2021

Aquaculture Reports

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Spoilage potential ofVagococcus salmoninarumin preservative-free, MAP-stored brown shrimp and differentiation fromBrochothrix thermosphactaon streptomycin thallous acetate actidione agar

Cited by 9 publications

References 42 publications

Novel members of bacterial community during a short-term chilled storage of common carp (Cyprinus carpio)

Novel members of bacterial community during a short-term chilled storage of common carp (Cyprinus carpio)

Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged broiler meat

Comparative analysis of gut bacterial community composition during a single day cycle in Chinese mitten crab (Eriocheir sinensis)

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