Spondias mombin: Quality control and anti-inflammatory activity in human neutrophils

In the present study, a polysaccharide from Ilex cornuta fruits (LCFP‐3) was obtained by hot water extraction, Diethyaminoethyl cellulose‐52 (DEAE‐52) chromatography column and Sephadex G‐100 gel column purification. Its structural characteristics were further explored using high performance anion exchange chromatography (HPAEC), gas chromatography and mass spectrometry (GC/MS), scanning electron microscopy (SEM), Fourier transform infrared (FT‐IR) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy. Monosaccharide composition analysis revealed LCFP‐3 contained mainly Galactose (31.92 %), Arabinose (25.87 %) and Galacturonic acid (23.35 %) while small percentage of Rhamnose, Glucose, Mannose and Xylose. Chemical composition analysis showed that the total sugar content of LCFP‐3 was 90.31 % and the protein content was 0.246 %. Gel permeation chromatography (GPC) analysis showed that its average molecular weight was 41.199 kDa. Structural analysis showed that LCFP‐3 may be composed of residues, T‐α‐Arap, T‐α‐Rhap, 1,3‐α‐Arap, 1,4‐α‐Arap, T‐β‐Galp, 1,4‐α‐GalpA(OMe), 1,4‐β‐Glcp, 1,3‐β‐Galp, 1,3,6‐β‐Manp, 1,6‐β‐Galp, 1,3,4‐β‐GalpA, 1,4,6‐β‐Manp, 1,3,6‐β‐Glcp, 1,2,3,4‐α‐Xylp. The anti‐inflammatory activity of LCFP‐3 was evaluated using lipopolysaccharide (LPS)‐induced RAW246.7 macrophages. The results showed that 1–200 μg/mL LCFP‐3 could dose‐dependently protect against LPS‐induced toxicity and 1 μg/mL LCFP‐3 could significantly inhibit LPS‐induced NO production. Therefore, LCFP‐3 exerted an anti‐inflammatory activity and has great potential as a functional ingredient.

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“…After 4 h, the solution was replaced with 150 μL DMSO to decompose formazan. The absorbance was measured at 490 nm with a microplate reader [39] …”

Section: Methodsmentioning

confidence: 99%

“…The absorbance was measured at 490 nm with a microplate reader. [39] Measurement of Nitric Oxide Production RAW246.7 macrophages were grouped and cultured according to cell culture and cell viability test. Cells were collected, lysed and centrifuged.…”

Section: Nuclear Magnetic Resonance Spectrummentioning

confidence: 99%

Isolation, Structural Analysis and Anti‐Inflammatory Activity of a Polysaccharide from Ilex cornuta Fruits

Cui

Tian

Sun

et al. 2022

Chemistry & Biodiversity

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“…A espécie Spondias mombin L., uma árvore conhecida como "cajazeira" no Nordeste do Brasil, além de seus frutos, suas folhas são bastante utilizadas na medicina tradicional por suas propriedades anti-inflamatórias, antimicrobianas e antivirais. Estudos mostraram que o extrato foi capaz de modular a ativação de neutrófilos, determinada pela redução da liberação da enzima mieloperoxidase pelos neutrófilos, o que pode desempenhar um papel importante na atividade anti-inflamatória in vivo da espécie (Silva et al, 2020).…”

Section: Plantas Medicinais Com Atividade Anti-inflamatóriaunclassified

“…Os compostos fenólicos são importantes devido às suas estruturas químicas, ideais para sequestro e neutralização de radicais (Moraes et al, 2020). Compostos fenólicos foram encontrados ao realizar o perfil cromatográfico de folhas de Spondias mombin, onde foi possível identificar e quantificar pela primeira vez o marcador bioativo geraniína, bem como a presença de outros compostos, como o ácido clorogênico que são responsáveis pela grande parte da sua atividade anti-inflamatória (Silva et al, 2020).…”

Section: Constituintes Químicos Responsáveis Pela Atividade Anti-inflamatóriaunclassified

Espécies medicinais do Brasil com potencial anti-inflamatório ou antioxidante: Uma revisão

Pereira

Martins

Rocha

et al. 2021

RSD

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As plantas medicinais são utilizadas pela população desde tempos imemoriais, representando uma fonte notável para o tratamento de diversas doenças humanas, que podem ter seu risco reduzido pela ingestão de produtos à base destas plantas, benefícios que são atribuídos a alguns metabólitos secundários que apresentam uma diversidade de atividades, dentre elas destacam-se a antiinflamatória e/ou antioxidante. O objetivo desse trabalho é realizar um levantamento bibliográfico buscando as espécies mais utilizadas de plantas medicinais do Brasil com potencial anti-inflamatório e antioxidante da última década. Foram utilizadas publicações indexadas nas bases de dados PubMed, Science Direct e Scielo nos últimos 10 anos, utilizando os seguintes descritores: “Brazilian medicinal plants”, “anti-inflammatory activity” e “antioxidant activity” Utilizando o marcador booleano “AND” assegurando a especificidade nas buscas. Foram identificados 1.185 artigos, onde 1.140 foram eliminados por não atenderem aos critérios de inclusão ou exclusão. O total de 45 artigos foram incluídos, categorizados de acordo com a utilização para atividade anti-inflamatória e atividade antioxidante. Dentre os compostos ativos com atividades anti-inflamatórias tem-se alguns compostos polifenólicos, além de alcalóide, terpenóide e esteróides. Estes metabólitos secundários atuam em diferentes alvos envolvidos no processo inflamatório. Os estudos têm demonstrado que os compostos polifenólicos, principalmente os flavonoides, apresentam propriedades antioxidantes que podem ser utilizados para fins terapêuticos em doenças como crônico-degenerativas. Esses resultados sugerem as perspectivas de estudos futuros para se obter uma melhor compreensão dos componentes individuais e o uso desses compostos como um possível agente terapêutico para patologias como a inflamação e aumento de radicais livres.

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“…Although the contributions of each marker to the pharmacological properties of the extracts from S. mombin have not yet been fully clarified, several biological activities, such as antioxidant, antiinflammatory, and gastroprotective, have been associated with phenolic constituents such as hydrolyzable tannins and flavonoids (Cabral et al, 2016;Pereira et al, 2020;Silva et al, 2020). Among the most cited properties for EA, the antioxidant activity against oxidative stress is notable.…”

Section: Chromatographic Markersmentioning

confidence: 99%

Optimization of the drying process of standardized extracts from leaves of Spondias mombin L. using Box–Behnken design and response surface methodology

Machado

Ferreira

Soares

2021

J. Food Process. Preserv.

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The extracts of Spondias mombin leaves were obtained to study the influence of drug amount and ethanol proportion on extract performance. The optimized extract was dried by spray drying, and the contributions were studied by Box-Behnken design to evaluate their influence on drying yield and product properties. The factorial data indicated the importance of both factors to the responses and the optimal conditions employing 10 g of herbal drug, and 80% ethanol was possible to recovery high amount of total polyphenols content (20.47 ± 0.08 g%), suitable dry residue (2.56 ± 0.04 g%), and great extraction efficient (8.10 ± 0.16%). Regarding the study of the drying process, the colloidal silicon dioxide provides spherical and larger particles with medium size 3.44 µm and moisture content equal to 4.15 ± 0.08% resulting in an increase in the physical characteristics of the dry products, such as yield (49.57%), CI (29.9%), and HR (1.43). Overall, the drying conditions (inlet temperature: 140°C and feed flow: 0.6 L/hr) provide lower hygroscopic powders, with yield of 44.66 ± 0.32% and moisture content 3.80 ± 0.06%. It was possible with the use of Box-Behnken design followed by response surface methodology that allowed the achievement of the optimum conditions for drying the standardized extract from leaves of S. mombin, to obtain powder beds with good flow and conservation of their phytochemicals. Practical applicationsConsidering the industrial production of vegetable inputs with antioxidant properties as an activity of high economic and social impact and conditioning the growth of this industrial segment to a vision of sustainable local development, it is essential that initiatives that promote the cultivation and processing of active vegetable inputs be encouraged as well as those that support the technological development of products with greater stability and easy handling. Spondias mombin appears as a species with high potential, as the species has a relevant participation in the rural economy through fruit production, the commercialization, and processing of its fruits.However, its leaves have bioactive compounds with important antioxidant activity, constituting a promising source for obtaining the substances involved in this activity, such as polyphenols. Thus, seeking to obtain products concentrated in polyphenols and improving the physical and chemical characteristics of this material is a promising How to cite this article: Machado JCB, Ferreira MRA, Soares LAL. Optimization of the drying process of standardized extracts from leaves of Spondias mombin L. using Box-Behnken design and response surface methodology. J Food

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Spondias mombin: Quality control and anti-inflammatory activity in human neutrophils

Cited by 10 publications

References 27 publications

Isolation, Structural Analysis and Anti‐Inflammatory Activity of a Polysaccharide from Ilex cornuta Fruits

Isolation, Structural Analysis and Anti‐Inflammatory Activity of a Polysaccharide from Ilex cornuta Fruits

Espécies medicinais do Brasil com potencial anti-inflamatório ou antioxidante: Uma revisão

Optimization of the drying process of standardized extracts from leaves of Spondias mombin L. using Box–Behnken design and response surface methodology

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