Sponge-Induced Angiogenesis in Mice and the Pharmacological Reactivity of the Neovasculature Quantitated by a Fluorimetric Method

Andrade, Sílvia Passos; Machado, R. D. P.; Teixeira, Andréia; Belo, Andrezza V.; Tarso, Alexandre M.; Beraldo, W. T.

doi:10.1006/mvre.1997.2047

Cited by 57 publications

(40 citation statements)

References 18 publications

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“…The sponge implantation model was developed to study angiogenesis in vivo as described previously with some modifications (48). Sponge discs (diameter, 0.7 cm; thickness, 0.2 cm) were prepared from the polyester polyurethane foam.…”

Section: Sponge Implantation Model Of Angiogenesismentioning

confidence: 99%

Nicotine Induces Cyclooxygenase-2 and Vascular Endothelial Growth Factor Receptor-2 in Association with Tumor-Associated Invasion and Angiogenesis in Gastric Cancer

Shin¹,

Wu²,

Chu³

et al. 2005

Molecular Cancer Research

107

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Blockade of angiogenesis is a promising strategy to suppress tumor growth, invasion, and metastasis. Vascular endothelial growth factor (VEGF), which binds to tyrosine kinase receptors [VEGF receptors (VEGFR) 1 and 2], is the mediator of angiogenesis and mitogen for endothelial cells. Cyclooxygenase-2 (COX-2) plays an important role in the promoting action of nicotine on gastric cancer growth. However, the action of nicotine and the relationship between COX-2 and VEGF/VEGFR system in tumorigenesis remain undefined. In this study, the effects of nicotine in tumor angiogenesis, invasiveness, and metastasis were studied with sponge implantation and Matrigel membrane models. Nicotine (200 Mg/mL) stimulated gastric cancer cell proliferation, which was blocked by SC-236 (a highly selective COX-2 inhibitor) and CBO-P11 (a VEGFR inhibitor). This was associated with decreased VEGF levels as well as VEGFR-2 but not VEGFR-1 expression. Topical injection of nicotine enhanced tumor-associated vascularization, with a concomitant increase in VEGF levels in sponge implants. Again, application of SC-236 (2 mg/kg) and CBO-P11 (0.4 mg/kg) partially attenuated vascularization by f30%. Furthermore, nicotine enhanced tumor cell invasion through the Matrigel membrane by 4-fold and promoted migration of human umbilical vein endothelial cells in a cocultured system with gastric cancer cells. The activity of matrix metalloproteinases 2 and 9 and protein expressions of plasminogen activators (urokinase-type plasminogen activator and its receptor), which are the indicators of invasion and migration processes, were increased by nicotine but blocked by COX-2 and VEGFR inhibitors. Taken together, our results reveal that the promoting action of nicotine on angiogenesis, tumor invasion, and metastasis is COX-2/VEGF/VEGFR dependent. (Mol Cancer Res 2005;3(11):607 -15)

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Section: Sponge Implantation Model Of Angiogenesismentioning

confidence: 99%

Nicotine Induces Cyclooxygenase-2 and Vascular Endothelial Growth Factor Receptor-2 in Association with Tumor-Associated Invasion and Angiogenesis in Gastric Cancer

Shin¹,

Wu²,

Chu³

et al. 2005

Molecular Cancer Research

107

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show abstract

“…FGF-2 has long been known to be a potent activator of angiogenesis in several types of angiogenic models in vivo, including chick chorioallantoic membrane assay (Ribatti et al, 1997;Wilting et al, 1991), rabbit, mouse, and rat corneal pocket assay (BenEzra et al, 1993;DeLisser et al, 1997;Kenyon et al, 1996;Seghezzi et al, 1998;Ziche et al, 1997), and sponge or Matrigel subcutaneous implant models in rodents (Andrade et al, 1997;BenEzra et al, 1993;Fan, 1995a, 1995b;Passaniti et al, 1992). VEGF has also been described as an angiogenic factor in vivo (Asahara et al, 1995;Dellian et al, 1996;Wilting et al, 1993), yet its potency does not appear to equate with FGF-2 in many of the assays used.…”

mentioning

confidence: 99%

Fibroblast Growth Factor 2 Activation of Stromal Cell Vascular Endothelial Growth Factor Expression and Angiogenesis

Claffey

Abrams²,

Shih

et al. 2001

Laboratory Investigation

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“…Vários estudos [28,[38][39][40][41][42][43][44][45] têm demonstrado a presença da resposta imune tumoral no momento do diagnóstico. Entretanto, ainda não há consenso sobre o real valor do infiltrado leucocitário no microambiente tumoral.…”

Section: Discussionunclassified

Estudo da resposta imune humoral em mulheres com neoplasia ovariana. -

Freitas¹

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Sponge-Induced Angiogenesis in Mice and the Pharmacological Reactivity of the Neovasculature Quantitated by a Fluorimetric Method

Cited by 57 publications

References 18 publications

Nicotine Induces Cyclooxygenase-2 and Vascular Endothelial Growth Factor Receptor-2 in Association with Tumor-Associated Invasion and Angiogenesis in Gastric Cancer

Nicotine Induces Cyclooxygenase-2 and Vascular Endothelial Growth Factor Receptor-2 in Association with Tumor-Associated Invasion and Angiogenesis in Gastric Cancer

Fibroblast Growth Factor 2 Activation of Stromal Cell Vascular Endothelial Growth Factor Expression and Angiogenesis

Estudo da resposta imune humoral em mulheres com neoplasia ovariana. -

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