Sponges of Carboxymethyl Chitosan Grafted with Collagen Peptides for Wound Healing

Abstract

Burns are physically debilitating and potentially fatal injuries. Two marine biomaterials, carboxymethyl chitosan (CMC) and collagen peptides (COP), have emerged as promising burn dressings. In this paper, sponges of carboxymethyl chitosan grafted with collagen peptide (CMC–COP) were prepared by covalent coupling and freeze drying. Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy were then used to characterize the prepared sponges. To evaluate the wound healing activity of the CMC–COP sponges, in vitro tests including cell viability scratch wound healing and scald wound healing experiments were performed in rabbits. Appearance studies revealed the porous nature of sponges and FTIR spectroscopy demonstrated the successful incorporation of COP into CMC. The in vitro scratch assay showed that treatment with CMC–COP sponges (at 100 μg/mL) had significant effects on scratch closure. For burn wounds treated with CMC–COP, regeneration of the epidermis and collagen fiber deposition was observed on day 7, with complete healing of the epidermis and wound on days 14 and 21, respectively. Based on the pathological examination by hematoxylin and eosinstaining, the CMC–COP group demonstrated pronounced wound healing efficiencies. These results confirmed that the CMC–COP treatment enhanced cell migration and promoted skin regeneration, thereby highlighting the potential application of these sponges in burn care.

Hybrid Freeze-Dried Dressings Composed of Epidermal Growth Factor and Recombinant Human-Like Collagen Enhance Cutaneous Wound Healing in Rats

“…With the improvements in recombinant protein techniques, the research on high-quality and contaminant-free RHC has been growing by leaps and bounds. The RHC may produce a suitable biological dressing for burn wounds as an alternative to native collagen (Shilo et al, 2013;Udhayakumar et al, 2017;Cheng and Hu, 2019). Rutschmann et al (2014) coexpressed human type III collagen with virus-derived proline 4-hydroxylase in the E. coli expression system to obtain RHC with hydroxylated structure (Rutschmann et al, 2014).…”

Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing

“…PLOS ONE •Sponges w/carboxymethyl chitosan and collagen peptides [167] •Gold nanoparticles (AuNP) [168] •Stromal vascular fraction (SVF) [169] •Quinone-based chromenopyrazole (QCP) antioxidant-laden silk fibroin electrospun nanofiber scaffold [170] •Pulmonary fibrosis associated RNA overexpression [171] •Titanium dioxide nanoparticle biofilm [172] •Neonatal cardiac fibroblasts infected with ETV2 [173] •exosomes platelet rich plasma [174] •tonsil derived Stem cell media [175] • [176] •Elaidic and linoleic (fatty acids) [119] •Light exposure [177] •Primary rat alveolar macrophages (AMO)-derived monocyte chemotactic protein-induced protein 1 (MCPIP1) knockdown [178] •Oxymatrine and Notch signaling pathway inhibitor (DAPT) via TGF-β1 [161]…”

Biocompatibility, hemostatic properties, and wound healing evaluation of tilapia skin collagen sponges

“…6 Soft spongy collagen can attach to different types of wound, absorb excess tissue exudate, reduce pain, and can promote the formation of epithelial and granulation tissue in a wound. 7 Over the past few years, collagen and collagen-based biomaterials have been widely used for rapid hemostasis, wound dressings, tissue engineering, and matrices for antibiotic delivery and have been successfully used clinically. 8,9 Many collagen-based wound dressing products have been developed, a number of which have been approved and are commercially available for hemostasis and the treatment of trauma, burns, chronic ulcers, and other wounds.…”

“…8,9 Many collagen-based wound dressing products have been developed, a number of which have been approved and are commercially available for hemostasis and the treatment of trauma, burns, chronic ulcers, and other wounds. 7,10 Collagen is principally sourced from the skin and bones of mammals such as cattle and pigs. However, a number of constraints have hindered the development of novel collagen products.…”