Spontaneous activity of cerebellar Purkinje cells and their responses to impulses in climbing fibres

Latham, A.; Paul, D. H.

doi:10.1113/jphysiol.1971.sp009373

Cited by 107 publications

(76 citation statements)

References 22 publications

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“…The over-all pattern of P cell discharge found here, consisting of a rather irregular discharge of simple spikes, punctuated by climbing fibre responses occurring at a mean rate in the region of 1/sec, is qualitatively similar to that found in numerous other investigations. However, the simple spike discharge was somewhat more irregular (as indicated by a larger coefficient of variation of the interspike intervals) and its mean rate (25/sec) was a little slower than has been reported for barbiturate anaesthetized (Bell & Grimm, 1969;Murphy & Sabah, 1970;Latham & Paul, 1971), de-cerebrate (Bloedel & Roberts, 1969;Murphy & Sabah, 1970) or unanaesthetized intact cats (Hobson & McCarley, 1972a, b;Marchesi & Strata, 1971; Armstrong & Rawson, 1976& Rawson, , 1979 and for unanaesthetized monkeys (Thach, 1968(Thach, , 1970Mano, 1970). In the present study, the administration of pentobarbitone temporarily stopped the P cell discharge, which was both slower and more regular when it resumed, as Murphy & Sabah (1970) found in decerebrate preparations.…”

Section: Comparison Of Purkinje Cell and Interpositus Neurone Responsesmentioning

confidence: 88%

Discharge patterns of Purkinje cells in cats anaesthetized with alpha‐chloralose.

Armstrong¹,

Cogdell²,

Harvey³

1979

The Journal of Physiology

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SUMMARY1. Micro-electrodes have been used to record from 119 Purkinje (P) cells in the paramedian lobule of the cerebellum in cats anaesthetized with c-chloralose.2. The spontaneous discharge rate and degree of irregularity of the discharge varied very much from one cell to another; the over-all mean rate (about 25/sec) was a little lower than has been reported either for barbiturate anaesthetized or for decerebrate unanaesthetized preparations.3. Following electrical stimulation of a peripheral nerve, most P cells responded with both simple spikes and a climbing fibre response. This initial response was usually succeeded by a prolonged period of silence (over-all mean duration 350 msec) before resumption of the tonic simple spike discharge. Similar response-silence sequences could also be evoked by mechanical stimulation such as a tap applied to the pads of the forepaw.4, Electrical stimulation of the inferior olive evoked climbing fibre responses followed by a prolonged pause in the simple spike discharge of the cell.5. In six individual preparations, recordings were made both from P cells of the paramedian lobule and from neurones of nucleus interpositus (to which the former project). Comparison of the responses of the two types of neurone to peripheral nerve and inferior olivary stimulation showed that the end of the pauses in P cell firing correlated well with the end of a prolonged period of facilitation of the interpositus neurones.6. These results support the hypothesis advanced in an earlier report (Armstrong, Cogdell & Harvey, 1975) that the prolonged facilitatory responses of interpositus neurones are essentially disinhibitory responses resulting from reduction in the activity of overlying cells, and that responses of P cells and of interpositus neurones consist, in general, of modulations of activity which are mutually out of phase.

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Section: Comparison Of Purkinje Cell and Interpositus Neurone Responsesmentioning

confidence: 88%

Discharge patterns of Purkinje cells in cats anaesthetized with alpha‐chloralose.

Armstrong¹,

Cogdell²,

Harvey³

1979

The Journal of Physiology

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“…However, preferential driving by synchronous inputs, as discussed above, could effectively result in the multiplexing of two information channels in the DCN inputs. The complex spike modulates simple spiking of Purkinje cells, e.g., by simple spike pauses after a complex spike (Latham and Paul, 1971;Armstrong and Rawson, 1979), which may in turn elicit rebound firing of DCN neurons (Aizenman and Linden, 1999) (but see Alviña et al, 2008). Synchronous CS activity across Purkinje cells may therefore result in activation of downstream neuronal populations in the DCN, with spatial activation patterns representing information at discrete time points marked out by subthreshold oscillations in the olive.…”

Section: Discussionmentioning

confidence: 99%

Spatial Pattern Coding of Sensory Information by Climbing Fiber-Evoked Calcium Signals in Networks of Neighboring Cerebellar Purkinje Cells

Schultz¹,

Kitamura²,

Post-Uiterweer³

et al. 2009

Journal of Neuroscience

130

152

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Climbing fiber input produces complex spike synchrony across populations of cerebellar Purkinje cells oriented in the parasagittal axis. Elucidating the fine spatial structure of this synchrony is crucial for understanding its role in the encoding and processing of sensory information within the olivocerebellar cortical circuit. We investigated these issues using in vivo multineuron two-photon calcium imaging in combination with information theoretic analysis. Spontaneous dendritic calcium transients linked to climbing fiber input were observed in multiple neighboring Purkinje cells. Spontaneous synchrony of calcium transients between individual Purkinje cells falls off over ϳ200 m mediolaterally, consistent with the presence of cerebellar microzones organized by climbing fiber input. Synchrony was increased after administration of harmaline, consistent with an olivary origin. Periodic sensory stimulation also resulted in a transient increase of synchrony after stimulus onset. To examine how synchrony affects the neural population code provided by the spatial pattern of complex spikes, we analyzed its information content. We found that spatial patterns of calcium events from small ensembles of cells provided substantially more stimulus information (59% more for seven-cell ensembles) than available by counting events across the pool without taking into account spatial origin. Information theoretic analysis indicated that, rather than contributing significantly to sensory coding via stimulus dependence, correlational effects on sensory coding are dominated by redundancy attributable to the prevalent spontaneous synchrony. The olivocerebellar circuit thus uses a labeled line code to report sensory signals, leaving open a role for synchrony in flexible selection of signals for output to deep cerebellar nuclei.

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“…Cerebellar Purkinje neurons have long been a focus of detailed electrophysiological study. Two striking features of Purkinje neurons in vivo are regular, spontaneous firing (Bell and Grimm, 1969;Latham and Paul, 1971) and, upon stimulation of climbing fibers, formation of "complex spikes" consisting of multiple peaks (Eccles et al, 1966(Eccles et al, ,1967Martinez et al, 1971). Work with in vitro preparations has suggested that both properties may depend, in part, on intrinsic membrane properties of Purkinje neurons.…”

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confidence: 99%

Resurgent Sodium Current and Action Potential Formation in Dissociated Cerebellar Purkinje Neurons

1997

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Voltage-dependent sodium channels were studied in dissociated cerebellar Purkinje neurons from rats. In whole-cell recordings, a tetrodotoxin ( TTX )-sensitive inward current was elicited when the membrane was repolarized to voltages between Ϫ60 and Ϫ20 mV after depolarizations to ϩ30 mV long enough to produce maximal inactivation. At Ϫ40 mV, this "resurgent" current peaked in 8 msec and decayed with a time constant of 30 msec. With 50 mM sodium as a charge carrier, the resurgent current was on average ϳ120 pA. CA3 pyramidal neurons had no such current. The current may reflect recovery of inactivated channels through open states, because in Purkinje neurons (but not CA3 neurons) there was partial recovery from inactivation at Ϫ40 mV, coinciding with the rise of resurgent current. In single-channel recordings, individual channels gave openings corresponding to resurgent and conventional transient current. Action potentials were recorded from dissociated neurons under current clamp to investigate the role of the resurgent current in action potential formation. Purkinje neurons fired spontaneously at ϳ30 Hz. Hyperpolarization to Ϫ85 mV prevented spontaneous firing, and brief depolarization then induced all-or-none firing of conglomerate action potentials comprising three to four spikes. When conglomerate action potentials were used as command voltages in voltageclamp experiments, TTX-sensitive sodium current was elicited between spikes. The falling phase of an action potential is similar to voltage patterns that activate resurgent sodium current, and thus, resurgent sodium current likely contributes to the formation of conglomerate action potentials in Purkinje neurons.

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Spontaneous activity of cerebellar Purkinje cells and their responses to impulses in climbing fibres

Cited by 107 publications

References 22 publications

Discharge patterns of Purkinje cells in cats anaesthetized with alpha‐chloralose.

Discharge patterns of Purkinje cells in cats anaesthetized with alpha‐chloralose.

Spatial Pattern Coding of Sensory Information by Climbing Fiber-Evoked Calcium Signals in Networks of Neighboring Cerebellar Purkinje Cells

Resurgent Sodium Current and Action Potential Formation in Dissociated Cerebellar Purkinje Neurons

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