Spontaneous and activation-induced apoptosis of peripheral blood mononuclear cells in the pathogenesis of type 1 diabetes mellitus

Луговая, А. В.; Калинина, Н. М.; Митрейкин, В. Ф.; Эмануэль, Ю. В.; Ковальчук, Ю. П.; Artemova, A V

doi:10.15789/1563-0625-saa-1834

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…The conditions we used to stimulate the cells allowed for developing a response both in the direction of amplification and suppression. Therefore, the negative effects associated with cellular memory, when the stimuli that activate resting T lymphocytes initiate apoptotic death in activated T lymphocytes [58,59], were minimized to be as low as possible. The model we have chosen allowed us to fulfill the main goal of the study: assess a difference between two types of HBsAg in their effect on immune cells.…”

Section: Discussionmentioning

confidence: 99%

Recombinant HBsAg of the Wild-Type and the G145R Escape Mutant, included in the New Multivalent Vaccine against Hepatitis B Virus, Dramatically Differ in their Effects on Leukocytes from Healthy Donors In Vitro

et al. 2022

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Immune-escape hepatitis B virus (HBV) mutants play an important role in HBV spread. Recently, the multivalent vaccine Bubo®-Unigep has been developed to protect against both wild-type HBV and the most significant G145R mutant. Here, we compared the effects of recombinant HBsAg antigens, wild-type and mutated at G145R, both included in the new vaccine, on activation of a human high-density culture of peripheral blood mononuclear cells (PBMC) in vitro. The antigens were used either alone or in combination with phytohemagglutinin (PHA). None of the antigens alone affected the expression of CD40, HLA-DR or CD279. Wild-type HBsAg enhanced CD86 and CD69 expression, and induced TNF-α, IL-10, and IFN-γ, regardless of the anti-HBsAg status of donor. In the presence of PHA, wild-type HBsAg had no effect on either of the tested surface markers, but increased IFN-γ and IL-10 and inhibited IL-2. In contrast, the G145R mutant alone did not affect CD86 expression, it induced less CD69, and stimulated IL-2 along with lowering levels of TNF-α, IL-10, and IFN-γ. The G145R mutant also suppressed PHA-induced activation of CD69. The dramatic differences in the immune responses elicited by wild-type HBsAg and the G145R mutant HBsAg suggest distinct adaptive capabilities of the G145R mutant HBV.

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Section: Discussionmentioning

confidence: 99%