Spontaneous and mitomycin-C induced sister-chromatid exchanges

Kram, David; Schneider, Edward L.; Senula, Gerhard C.; Nakanishi, Yoshifumi

doi:10.1016/0027-5107(79)90024-1

Cited by 30 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed values range from 0.75 to 1.6 SCEs per cell per generation in various rodent tissues (Tice et al, 1976, Kanda andKato, 1979;Kram et al, 1979;Sutuo, 1981). Even the highest of these values is lower than our observations using peripheral lymphocytes.…”

Section: Discussioncontrasting

confidence: 56%

Determination of the baseline sister chromatid exchange frequency in human and mouse peripheral lymphocytes using monoclonal antibodies and very low doses of bromodeoxyuridine

Tucker

Christensen

Strout

et al. 1986

Cytogenet Genome Res

View full text Add to dashboard Cite

We measured the frequency of sister chromatid exchanges (SCEs) in human and mouse peripheral lymphocytes using doses of bromodeoxyuridine (BrdU) ranging from 30 ΠM to 100 µM (human) and from 10 nM to 10 µM (mouse). Heparinized peripheral blood was obtained from five healthy nonsmokers and from six C57B1/6 male mice. The blood was stimulated with PHA (human) or lipopolysaccharide (LPS, mouse) and grown for the first of two cell cycles in BrdU. Metaphase chromosomes were denatured and exposed to a monoclonal antibody reactive to single-stranded DNA containing BrdU. A second antibody was used to label the first antibody with fluorescein, and propidium iodide was used as a counterstain. Second-division metaphases were thus differentially stained red to indicate DNA content and yellow-green to indicate the presence of BrdU. The results indicate that the baseline SCE frequency in human and mouse peripheral lymphocytes is 3.6 and 2.4 SCEs per cell per generation, and that in the human these frequencies are invariant at the lowest BrdU levels. This suggests that SCEs are an integral part of DNA replication, even in the absence of agents known to induce SCEs. The distribution of SCEs per chromosome was analyzed and found to be Poisson-distributed in all 24 murine cultures and in 25 of 36 human cultures. The distribution of SCEs per chromosome may be due to either species-specific chromosome packaging or to karyotypic differences between the species.

show abstract

Section: Discussioncontrasting

confidence: 56%

Determination of the baseline sister chromatid exchange frequency in human and mouse peripheral lymphocytes using monoclonal antibodies and very low doses of bromodeoxyuridine

Tucker

Christensen

Strout

et al. 1986

Cytogenet Genome Res

View full text Add to dashboard Cite

show abstract

“…While we also noted a slight trend toward increased spontaneous SCE levels in late passage cells, the differences were not sta tistically significant. These spontaneous in vitro SCE levels, 7.4-S.6 SCE/second cycle cell or 3.7-4.3 SCE/cell per cell cycle, are approximately four-fold higher than spontaneous in vivo SCE levels (Tin: et al, 1976;Kram et al, 1979). There are several possible explana tions for this observed difference.…”

mentioning

confidence: 93%

“…However, an estimate of the spontaneous frequencies of SCE can be obtained from examinations of the frequen cies of SCE over a range of BrdU concen trations. Studies of spontaneous SCE levels (Tin et al, 1976;Kram et al, 1979).…”

mentioning

confidence: 99%

Aging and sister chromatid exchange

El¹,

Ck²

1980

Cytogenet Genome Res

View full text Add to dashboard Cite

“…Advances in bromodeoxyuridine (BrdUrd)-fluorescent dye-Giemsa methodology for the discrimination of sister chromatids allowed a finer resolution of SCEs [lo, 16,22,26]. However, increasing doses of BrdtJrd have been shown t o increase SCE frequencies and chromosomal aberrations in vitro [15,21,24, 361 and in vivo [19,33]. Furthermore, as the percentage of BrdUrd substitution in DNA increased in CHO cells, SCE induction also increased in a linearly proportional manner [24] .…”

Section: Ntrod Uctl Onmentioning

confidence: 99%

Sister chromatid exchange in vivo in mice: I. The influence of increasing doses of bromodeoxyuridine

Wilmer

Soares

1980

Environ. mutagen

View full text Add to dashboard Cite

Bromodeoxyuridine (BrdUrd) pellets weighting either 20, 25, 30, 40, 45, or 53 mg were implanted subcutaneously in strain DBA/2J male mice. Femoral bone marrow cels were analyzed for sister chromatid exchange (SCE) and chromosome aberration frequencies, mitotic induces, and cellular replication kinetics. Small but statistically significant BrdUrd dose-dependent increases of SCEs were observed, whereas chromosome aberrations were induced at low frequencies and occurred independently in Brd Urd dose. The mitotic index remained relatively constant for all doses. A slight inhibition of cellular replication, as manifested by a reduction in third division cells at the 40- and 45-mg doses, was observed. The use of an intense fluorescent light source in the fluorescence-plus-Giemsa technique provided consistently good sister chromatid differentiation at pellet doses substantially lower than those previously used by other investigators.

show abstract

Spontaneous and mitomycin-C induced sister-chromatid exchanges

Cited by 30 publications

References 28 publications

Determination of the baseline sister chromatid exchange frequency in human and mouse peripheral lymphocytes using monoclonal antibodies and very low doses of bromodeoxyuridine

Determination of the baseline sister chromatid exchange frequency in human and mouse peripheral lymphocytes using monoclonal antibodies and very low doses of bromodeoxyuridine

Aging and sister chromatid exchange

Sister chromatid exchange in vivo in mice: I. The influence of increasing doses of bromodeoxyuridine

Contact Info

Product

Resources

About