Spontaneous deletions and flanking regions of the chromosomally inherited hemolysin determinant of an Escherichia coli O6 strain

Hacker, Jörg; Knapp, S.; Goebel, Werner

doi:10.1128/jb.154.3.1145-1152.1983

Cited by 113 publications

(56 citation statements)

References 27 publications

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“…We then subcloned the 5.3-kb BamHI-EcoRI fragment, which spans the region 0.2 kb left of P10 to the middle of P8 (Fig. 4), into pBR322 (pANN801-11 13. Transformation of E. coli K-12 HB101 cells with this plasmid resulted in the production of fimbriae and mannoseresistant hemagglutination.…”

Section: Resultsmentioning

confidence: 99%

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Cloning and characterization of genes involved in production of mannose-resistant, neuraminidase-susceptible (X) fimbriae from a uropathogenic O6:K15:H31 Escherichia coli strain

Hacker¹,

Schmidt²,

Hughes³

et al. 1985

Infect Immun

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174

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The qropathogenic Escherichia coli strain 536 (06:K15:H31) exhibits a mannose-resistant hemagglutination phenotype (Mrh) with bovine erythrocytes and delayed Mrh with human and guinea pig erythrocytes. Neuraminidase treatment of the erythrocytes abolishes mannose resistant hemagglutination, which is typical for X fimbriae. E. coli strain 536 synthesizes two different fimbriae (Fim phenotype) protein subunits, 16.5 and 22 kilodaltons in size. In addition the strain shows mannose-sensitive hemagglutination and common type I (F1) fimbriae. The cosmid clone E. coli K-12(pANN801) and another nine independently isolated Mrh+ cosmid clones derived from a cosmid gene bank of strain 536 express the 16.5-kilodalton protein band, but not the 22-kilodalton protein, indicating an association of the Mrh+ property with the "16.5-kilodalton fimbriae." All cosmid clones were fimbriated, and they reacted with antiserum produced against Mrh+ fimbriae of the E. coli on July 11, 2020 by guest http://iai.asm.org/ Downloaded from Golecki, and M. Kist. 1980. Characterization of pili associated with Escherichia coli 018ac. Infect. Immun. 29:685-691. INFECT. IMMUN. on July 11, 2020 by guest http://iai.asm.org/ Downloaded from

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Section: Resultsmentioning

confidence: 99%

“…Isolation of chromosomal and plasmid DNA. Chromosomal DNA was isolated as described previously (13). Plasmid DNA from clones carrying recombinant DNA was screened by the cleared lysate procedure (4), and preparative DNA isolation was achieved as described previously (11).…”

Section: Methodsmentioning

confidence: 99%

Cloning and characterization of genes involved in production of mannose-resistant, neuraminidase-susceptible (X) fimbriae from a uropathogenic O6:K15:H31 Escherichia coli strain

Hacker¹,

Schmidt²,

Hughes³

et al. 1985

Infect Immun

Self Cite

174

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“…The first intensively studied Pais were those which are part of the genomes of uropathogenic and enteropathogenic E. coli strains (Hacker et a/., 1983;Low et a/., 1984;Muhldorfer and Hacker, 1994;McDaniels et a/., 1995;Blum et a/., 1994;Swenson et a/., 1996). The uropathogenic E. coli strains 536 (06:K15) and J96 (04:K6) carry Pais with sizes of 70-190 kb (see Table I), The Pais of strain 536, indicated in Fig.…”

Section: Pathogenicity Islands Of E Colimentioning

confidence: 99%

Pathogenicity islands of virulent bacteria: structure, function and impact on microbial evolution

Hacker¹,

Blum-Oehler²,

Mühldorfer³

et al. 1997

Molecular Microbiology

1,016

738

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Summary Virulence genes of pathogenic bacteria, which code for toxins, adhesins, invasins or other virulence factors, may be located on transmissible genetic elements such as transposons, plasmids or bacteriophages. In addition, such genes may be part of particular regions on the bacterial chromosome, termed‘pathogenicity islands’(Pais). Pathogenicity islands are found in Gram‐negative as well as in Gram‐positive bacteria. They are present in the genome of pathogenic strains of a given species but absent or only rarely present in those of non‐pathogenic variants of the same or related species. They comprise large DNA regions (up to 200 kb of DNA) and often carry more than one virulence gene, the G+C contents of which often differ from those of the remaining bacterial genome. In most cases, Pais are flanked by specific DNA sequences, such as direct repeats or insertion sequence (IS) elements. In addition, Pais of certain bacteria (e.g. uropathogenic Escherichia coli, Yersinia spp., Helicobacter pylori) have the tendency to delete with high frequencies or may undergo duplications and amplifications. Pais are often associated with tRNA loci, which may represent target sites for the chromosomal integration of these elements. Bacteriophage attachment sites and cryptic genes on Pais, which are homologous to phage integrase genes, plasmid origins of replication or IS elements, indicate that these particular genetic elements were previously able to spread among bacterial populations by horizontal gene transfer, a process known to contribute to microbial evolution.

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“…E. coli CFT073 was isolated from the blood and urine of a hospitalized patient with acute pyelonephritis and urosepsis (78) and E. coli 536 was isolated from a patient with pyelonephritis (79). Transurethral infections with CFT073 or 536 were performed in mice immunized with vaccine formulations containing Hma, IreA, IutA, or FyuA.…”

Section: Methodsmentioning

confidence: 99%

Optimization of an Experimental Vaccine to PreventEscherichia coliUrinary Tract Infection

Forsyth

Himpsl

Smith

et al. 2020

Preprint

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AbstractUrinary tract infections (UTI) affect half of all women at least once during their lifetime. The rise in extended-spectrum beta-lactamase-producing strains and potential for carbapenem resistance within uropathogenic Escherichia coli (UPEC), the most common causative agent of UTIs, creates an urgent need for vaccine development. Intranasal immunization of mice with UPEC outer membrane iron receptors, FyuA, Hma, IreA, or IutA, conjugated to cholera toxin, provides protection in the bladder or kidneys when challenged with UPEC CFT073 or 536. Based on these data, we sought to optimize the vaccination route (intramuscular, intranasal, or subcutaneous) in combination with adjuvants suitable for human use including alum, monophosphoryl lipid A (MPLA), unmethylated CpG synthetic oligodeoxynucleotides (CpG), polyinosinic:polycytodylic acid (polyIC), and mutated heat-labile E. coli enterotoxin (dmLT). Mice intranasally vaccinated with dmLT-IutA or dmLT-Hma displayed a significant reduction in bladder colonization (86-fold and 32-fold, respectively) with 40–42% of mice having no detectable colony forming units (CFU). Intranasal vaccination of mice with CpG-IutA and polyIC-IutA significantly reduced kidney colonization (131-fold) and urine CFU (22-fold), respectively. dmLT generated the most consistently robust antibody response in intranasally immunized mice, while MPLA and alum produced greater concentrations of antigen-specific serum IgG with intramuscular immunization. Based on these results, we conclude that intranasal administration of Hma or IutA formulated with dmLT adjuvant provides the greatest protection from UPEC UTI. This study advances our progress toward a vaccine against uncomplicated UTI, which will significantly improve the quality of life for women burdened by recurrent UTI and enable better antibiotic stewardship.ImportanceUrinary tract infections (UTI) are among the most common bacterial infection in humans, affecting half of all women at least once during their lifetimes. The rise in antibiotic resistance and health care costs emphasizes the need to develop a vaccine against the most common UTI pathogen, Escherichia coli. Vaccinating mice intranasally with a detoxified heat-labile enterotoxin and two surface exposed receptors, Hma or IutA, significantly reduced bacterial burden in the bladder. This work highlights progress in the development of a UTI vaccine formulated with adjuvants suitable for human use and antigens that encode outer membrane iron receptors required for infection in the iron-limited urinary tract.

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Spontaneous deletions and flanking regions of the chromosomally inherited hemolysin determinant of an Escherichia coli O6 strain

Cited by 113 publications

References 27 publications

Cloning and characterization of genes involved in production of mannose-resistant, neuraminidase-susceptible (X) fimbriae from a uropathogenic O6:K15:H31 Escherichia coli strain

Cloning and characterization of genes involved in production of mannose-resistant, neuraminidase-susceptible (X) fimbriae from a uropathogenic O6:K15:H31 Escherichia coli strain

Pathogenicity islands of virulent bacteria: structure, function and impact on microbial evolution

Optimization of an Experimental Vaccine to PreventEscherichia coliUrinary Tract Infection

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