Spontaneous differentiation of mesenchymal stem cells obtained from fetal rat circulation

Naruse, Keiko; Urabe, Ken; Mukaida, Tomoyuki; Ueno, Takeshi; Migishima, Fujio; Oikawa, Astuhiko; Mikuni-Takagaki, Yuko; Itoman, Moritoshi

doi:10.1016/j.bone.2004.05.006

Cited by 25 publications

(20 citation statements)

References 34 publications

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“…roid hormone receptor) were expressed not only in osteogenically differentiated (Runx-2 and bone sialoprotein positive), but also in undifferentiated cells (Fig. 6C), previous findings showed that osteonectin was expressed in bone marrow-derived (undifferentiated) MSCs (Silva et al, 2003), and MSCs in fetal circulating blood spontaneously became PTHR-positive cells (Naruse et al, 2004). Therefore, our findings do not necessarily deny the ability of 5F9A cells to undergo osteoblastic differentiation.…”

Section: F9a Cells Are Oligopotentsupporting

confidence: 44%

Oligopotent Mesenchymal Stem Cell‐Like Clone Becomes Multinucleated Following Phorbol Ester, TPA Stimulation

Yoshida

Ono

Maejima

et al. 2007

The Anatomical Record

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We established a mesenchymal stem cell clone, 5F9A, from rat bone marrow substrate adherent cells by repeated limiting dilutions. The cells have a fibroblastic shape and form intimate contacts with adjacent cells with interdigitations and junctions similar to adherence and tight junctions in a semi-confluent culture. Analysis of the phenotypes of these cells by RT-PCR and FACS demonstrated that they resembled mesenchymal stem cells, and the cells could differentiate into adiopocytes and osteoblasts under appropriate conditions in vitro showing their oligopotency. Furthermore, the cells were induced to become multinuclear cells by TPA (12-o-tetradecanoylphorbol 13-acetate) stimulation.

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Section: F9a Cells Are Oligopotentsupporting

confidence: 44%

Oligopotent Mesenchymal Stem Cell‐Like Clone Becomes Multinucleated Following Phorbol Ester, TPA Stimulation

Yoshida

Ono

Maejima

et al. 2007

The Anatomical Record

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“…Significantly, this increment is moderately definite for MSCs, while HPCs exhibited no or limited increase under hypoxic situations. Some experiments determined cells similar to BM MSCs to circulate in peripheral blood from humans and animals, [88][89][90][91] while other studies led to contrary conclusions. 92,93 MSCs can be distinguished directly or indirectly in peripheral blood grafts after such a mobilization process, as several experiments have demonstrated.…”

mentioning

confidence: 99%

The Effect of Hypoxia on Mesenchymal Stem Cell Biology

Ejtehadifar¹,

Shamsasenjan²,

et al. 2015

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“…This was related to a spontaneous differentiation of the stem cells. [30] Additionally, the expression of connexin 32 as a gap junction protein was possibly favored by the development of cell monolayers and thus closer cell to cell contacts.…”

Section: Resultsmentioning

confidence: 99%

Co‐Cultures of Primary Cells on Self‐Supporting Nanoporous Alumina Membranes

et al. 2010

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Due to their unique properties, self‐supporting nanoporous aluminum oxide (alumina) membranes are useful substrates for the indirect co‐cultivation of cells. The membrane can act as a physical barrier between different cell types, whereas the cell‐to‐cell communication is guaranteed by the diffusion of soluble molecules or factors through the pores. With the help of such membranes the mRNA expression of hepatic genes can be induced in human adipose‐derived mesenchymal stem cells (hASCs) during an indirect co‐cultivation with primary mouse hepatocytes under different culture conditions. This proof of concept shows that such a cultivation approach is beneficial for different issues in the field tissue engineering or cell therapy.

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Spontaneous differentiation of mesenchymal stem cells obtained from fetal rat circulation

Cited by 25 publications

References 34 publications

Oligopotent Mesenchymal Stem Cell‐Like Clone Becomes Multinucleated Following Phorbol Ester, TPA Stimulation

Oligopotent Mesenchymal Stem Cell‐Like Clone Becomes Multinucleated Following Phorbol Ester, TPA Stimulation

The Effect of Hypoxia on Mesenchymal Stem Cell Biology

Co‐Cultures of Primary Cells on Self‐Supporting Nanoporous Alumina Membranes

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