Spontaneous epizootic of pneumococcus infection in guinea-pigs

Keyhani, Massoud; Naghshineh, R

doi:10.1258/002367774780943977

Cited by 10 publications

(7 citation statements)

References 3 publications

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“…Streptococcus pneumoniae, particularly type 19 (Keyhani.& Naghshineh, 1974), is a well-recognized pathogen for guinea-pigs, causing septicaemia with Sebesteny exudative pleuropneumonia and pericarditis, either alone or in conjunction with other, secondary invaders. These latter may well overgrow pneumococci, especially in tissue fluids or liquid cultures.…”

Section: Discussionmentioning

confidence: 99%

The isolation of carbon dioxide-requiring (carboxyphilic) type 19 pneumococcus (Streptococcus pneumoniae) from diseased guinea-pigs

Sebesteny

1978

Lab Anim

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SummaryType 19 pneumococcus was isolated only in anaerobic cultures from tissue fluids of guinea-pigs suffering from exudative pericarditis and pleuropneumonia. The organism was pathogenic to mice even after 40 subcultures. Further study showed it to be carboxyphilic rather than anaerobic. The necessity of specifying various atmospheric conditions in the diagnostic bacteriological isolation routine is emphasized.In the experimental guinea-pig stock of a neighbouring institute, purchased from a commercial source, sporadic mortality and morbidity associated with emaciation was observed throughout a year. On postmortem examination exudative pericarditis and pleuropneumonia was seen in most cases. Our laboratory was asked to assist in the investigation of the cause of this condition. Livers from 2, heart blood from 5, pericardial fluid from 2 and pleural fluid from 2 cases were taken with aseptic precautions and submitted to us for bacteriological examination. IsolationThe livers were individually scissor-minced, and loapfuls from the resulting tissue mince were seeded on 7% layered horse-blood agar plates prepared from Oxoid Blood Agar Base No.2 (CM271; Oxoid Ltd, Wade Road, Basingstoke, Hampshire, RG240PW) spread and incubated aerobically and anaerobically at 37°C. Loopfuls of pericardial and pleural fluids were similarly plated and incubated. Heart-blood samples were presented in nutrient broth (Oxoid CM2) and cooked-meat (Oxoid CM82) media which were-e incubated for 24 h at 37°C and subsequently subcul· tured on blood-agar plates and incubated aerobically and anaerobically at 37°C. For anaerobic incubation a mixture of 95% hydrogen and 5% carbon dioxide was used.Fluid samples were also subjected to microscopical examination. R~suJtsHanging drop preparations of one of the pericardial fluid samples 'showed numerous pairs of cocci either individually or in chains which were gram-positive on

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Section: Discussionmentioning

confidence: 99%

The isolation of carbon dioxide-requiring (carboxyphilic) type 19 pneumococcus (Streptococcus pneumoniae) from diseased guinea-pigs

Sebesteny

1978

Lab Anim

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“…ETIOLOGY Streptococcus pneumoniae is an oval-to lancet-shaped, encapsulated coccus that presents in pairs (diplococcus) or short chains (Fox, 2002;Percy and Barthold, 2007). S. pneumoniae is an α-hemolytic streptococcus that ferments trehalose and is optochin-sensitive (Harkness and Wagner, 1995;Keyhani and Naghshineh, 1974;Songer and Post, 2005). Additionally, it is not categorized according to one of the Lancefield groupings (Songer and Post, 2005).…”

Section: Streptococcus Pneumoniaementioning

confidence: 99%

“…Abortions and stillbirths can accompany a high mortality rate during an epizootic outbreak in a guinea pig colony (Percy and Barthold, 2007). CLINICAL MANIFESTATIONS In 1974, a spontaneous epizootic of respiratory infection involving 2400 guinea pigs was caused by S. pneumoniae type 19 (Keyhani and Naghshineh, 1974). During this outbreak, 450 guinea pigs died within 33 days of infection, and a short course of listlessness and failure to thrive were the only noted clinical signs prior to death.…”

Section: Streptococcus Pneumoniaementioning

confidence: 99%

Infectious Diseases

Brabb

Newsome

Burich

et al. 2012

The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents

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“…Although the organism is commonly found in the respiratory tracts of normal animis of these species [3, 6, 13] and healthy people [4] [5,6,13,15] but seemed to be sporadic these some decades [7,14,16].…”

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confidence: 99%

“…4 It is known that S. pneumoniae has at least 83 different serotypes, and types 19, 6, 14, 23, 3, 7 and 18 were commonly found in human being [9]. On the other hand, many investigators [5,7,13,15,16] reported epizootics of type 19 pneumococcal infection in guinea pigs. Although serotyping was not made on our pneumococcal strain, it must be important to study the possibility of transmission of the organism between human being and guinea pigs from standpoints of zoonoses.…”

mentioning

confidence: 99%