Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells-III

Dean, D. Metcalfe; Pross, Hugh F.; Kennedy, James C.

doi:10.1016/0360-3016(78)90186-4

Cited by 27 publications

(7 citation statements)

References 25 publications

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“…The apparent lack of direct dose-response relationship and the delayed as well as slow recovery of activity strongly suggest the involvement of regulatory cells. Relative radioresistance of NK activity and evidence for cell interactions normally resulting in inhibition of cytotoxicity were also reported by Dean et al (1978) for a human NK system.…”

mentioning

confidence: 70%

Do Natural Killer Cells Engage in Regulated Reactions Against Self to Ensure Homeostasis?

Cudkowicz

Hochman

1979

Immunological Reviews

267

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Host reactivities not requiring immunization in the mouse, especially natural resistance of irradiated animals to accept grafts of normal or malignant hemopoietic cells, were compared with NK activity against the YAC-1 lymphoma. The effects of several independent variables known to influence natural resistance in vivo had a similar effect on the NK system. Figure 12 lists an impressive array of shared properties and positive correlations. In contrast, the distinctions were few and minor. Many of the positive correlations were of particular significance since the experimental variables either have opposing or no effects on conventional induced immunity. The multiplicity and pervasiveness of these correlations suggest that the cellular mechanisms underlying natural reactivities are similar or common. Cytotoxic effectors mediating natural resistance to normal cells, tumors, and cells infected with intracellular pathogens may be distinct in terms of target selectivity, yet belong to a single cell lineage subject to common regulatory influences for differentiation and function. Regulation of reactivity via suppressor cells was studied in the NK system only. The spleens of mice selected for low levels of NK activity (resulting from young age, irradiation, and treatment with the macrophage-active agents l-carrageenan or hydrocortisone acetate) contained cells capable of inhibiting the lytic function of NK effectors taken from untreated adult donors. All the suppressor cells studied were thymus-independent, as judged by their occurrence in spleens of genetically athymic mice; the suppressive function was resistant to 2000 rads of gamma-rays administered in vitro and was not restricted by the major histocompatibility complex, without exception. However, two major classes of suppressors were identified: (a) macrophagelike cells inducible by l-carrageenan or hydrocortisone acetate, and (b) nonadherent cells found in spleens of untreated infants and of irradiated adult mice. It is proposed that the suppression of NK cytolysis demonstrated in vitro was a manifestation of regulatory mechanisms modulating the level of NK activity in vivo. Macrophagelike cells that are induced, activated, or inactivated by bacteria, viruses, hormones, and other agents may act as regulators of differentiation, maturation, and function of cells belonging to the NK lineage. Nonadherent cells could be either a distinct class of suppressors or immature NK cells capable of binding but not lysing target cells. In the latter case, regulation would be achieved via competitive binding of targets by pre-NK cells presumably in dynamic equilibrium with functional (i.e. matured) NK effectors.

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mentioning

confidence: 70%

Do Natural Killer Cells Engage in Regulated Reactions Against Self to Ensure Homeostasis?

Cudkowicz

Hochman

1979

Immunological Reviews

267

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“…Trypsinization causes some reduction in cytotoxicity (Jondal and Pross, 1975;Peter et al, 1975b), in contrast to neuraminidase treatment (Jondal and Pross, 1975). The cytotoxic activity of the cell is relatively radioresistant, has a D o of 750-850 rads, and may be stimulated by low dose irradiation (500-1000 rads) (Dean et al, 1977). The cytotoxic activity of the cell is relatively radioresistant, has a D o of 750-850 rads, and may be stimulated by low dose irradiation (500-1000 rads) (Dean et al, 1977).…”

Section: H F Pross and M G Baines: A Review Of Slmcmentioning

confidence: 99%

Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells

Pross

Baines

1977

Cancer Immunol Immunother

129

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“…Another characteristic of human NK cells is relative radioresistance; in fact stimulation of NK cells activity was observed after irradiation in the dose range of 500-1000 rads (Dean et al, 1977). In our own experiments, we have also found the NK cell activity of human peripheral blood to be radioresistant (Lotzov~t et al, unpublished data).…”

Section: Characteristics Of Nk Cellsmentioning

confidence: 68%