Spontaneous Localization of Particles in Giant Vesicles Owing to Depletion Force

Natsume, Yuno; Noguchi, Erika; Kurihara, Kensuke

doi:10.7566/jpsj.88.033001

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…As a GUV provides a closed space, the encapsulated polymer and particles exhibit entropically driven phenomena. Natsume et al reported that when micrometer-sized polystyrene particles of two sizes are encapsulated in a GUV, size-dependent localization of the particle distribution occurs [81]. The GUV contained several hundred large particles, whose volume fraction exceeded that of the small particles.…”

Section: Encapsulation Of Micrometer-sized Particlesmentioning

confidence: 99%

A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets

2023

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Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than 1 μm, have attracted attention not only as model cell membranes but also for the construction of artificial cells. For encapsulating water-soluble materials and/or water-dispersible particles or functionalizing membrane proteins and/or other synthesized amphiphiles, giant unilamellar vesicles (GUVs) have been applied in various fields, such as supramolecular chemistry, soft matter physics, life sciences, and bioengineering. In this review, we focus on a preparation technique for GUVs that encapsulate water-soluble materials and/or water-dispersible particles. It is based on the centrifugation of a water-in-oil emulsion layered on water and does not require special equipment other than a centrifuge, which makes it the first choice for laboratory use. Furthermore, we review recent studies on GUV-based artificial cells prepared using this technique and discuss their future applications.

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Section: Encapsulation Of Micrometer-sized Particlesmentioning

confidence: 99%

A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets

2023

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“…A good example of their approach is cell-mimicking giant vesicles (GV) that proliferate from generation to generation (Kurihara et al 2015). Polystyrene beads encapsulated in the giant vesicles reveal a unique spatial distribution: smaller beads come closer to the vesicle (Natsume et al 2019). They extended a typical statistical mechanics theory to explain this unique distribution as well as the frequent division of particle-containing vesicles.…”

Section: Biophysical Research At Imsmentioning

confidence: 99%