Spontaneous Lymphoblastoid Transformation of Cells Obtained from Human Tonsils

Abstract

Spontaneous lymphoblastoid transformation of cells obtained from 25 human tonsils were investigated. Three Epstein-Barr virus (EBV) genome-positive lymphoblastoid cell lines were spontaneously established in tissue cultures in vitro from human tonsils. In addition, the frequency of spontaneous lymphoblastoid transformation was increased by the treatment of tumor promoter, 12-0-tetradecanoylphorbol-13-acetate and 18 lymphoblastoid cell lines were established. The data suggest that the human tonsil is a reservoir for EBV.

A global phylogenetic analysis of Japanese tonsil-derived Epstein–Barr virus strains using viral whole-genome cloning and long-read sequencing

“…A previous study demonstrated that EBV-infected sLCLs were obtained from tonsillar tissues by bulk cultivation of minced tonsillar tissues in medium supplemented with 12- o -tetradecanoylphorbol 13-acetate (TPA) [16]. We applied the protocol to tonsillar tissues obtained from 17 patients undergoing routine tonsillectomies.…”

“…Tonsil-derived sLCLs were established according to a previous study [16]. Briefly, tonsillar tissues were minced, and red blood cells were removed by ACK lysis buffer (0.15 M NH 4 Cl, 10 mM KHCO 3 and 1 mM EDTA-2Na) treatment.…”

“…In this study, we aimed to characterize Japanese asymptomatically infected EBV strains. We chose sLCLs derived from human tonsillar tissues as a source of EBV DNA, as tonsillar tissues are known to be the reservoirs of EBV latent infection [16]. Asymptomatically infected EBV strains derived from tonsillar tissues of Japanese individuals were cloned into BAC vectors.…”

Rapid CRISPR/Cas9-Mediated Cloning of Full-Length Epstein-Barr Virus Genomes from Latently Infected Cells

“…Naturally arising LCLs are available from various sources. For example, they can be established from peripheral blood [ 42 ] or from tonsillar tissues obtained by routine tonsillectomy [ 43 ]. These naturally arising LCLs can serve as ideal resources to investigate EBV strain variation among individuals.…”

Human Lymphoblastoid Cell Lines in Pharmacogenomics

“…However, spontaneous cellular immortalization has been observed in human somatic cells from patients with genetic disorders [1][2][3][4]. The transformation efficiency of spontaneous lymphoblastoid cell lines (SP-LCLs) was increased by tumor promoter treatment [8], radiation [9], and sometimes in cyclosporine-containing medium from EBV-seropositive patients [10]. The transformation efficiency of spontaneous lymphoblastoid cell lines (SP-LCLs) was increased by tumor promoter treatment [8], radiation [9], and sometimes in cyclosporine-containing medium from EBV-seropositive patients [10].…”