Spontaneous Production of lnterleukin-8 by Human Lung Cancer Cells and Its Augmentation by Tumor Necrosis Factor Alpha and lnterleukin-1 at Protein and mRNA Levels

Mizuno, Kazuto; Sone, Saburo; Orino, Etsuko; Mukaida, Naofumi; Matsushima, Kouji; Ogura, Takeshi

doi:10.1159/000227385

Cited by 22 publications

(11 citation statements)

References 15 publications

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“…Compared with normal lung tissue, increased quantities of IL-8 were detected in bronchogenic carcinoma tissue [5]. IL-8 is also spontaneously produced by human lung cancer cells, suggesting that tumor-derived IL-8 may be important in recruiting inflammatory neutrophils and promoting interaction between lung cancer and inflammatory cells [18].…”

Section: Discussionmentioning

confidence: 99%

Proinflammatory Cytokine Levels in Patients with Lung Cancer and Carcinomatous Pleurisy

et al. 1998

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Increased levels of interleukin-6 (IL-6) and interleukin-8 (IL-8) have been reported in various diseases, including lung cancer. The role of the soluble form of the IL-6 receptor (sIL-6R) remains to be explored. We therefore measured IL-6, IL-8 and sIL-6R in effusion fluid and blood serum of 10 lung cancer patients with carcinomatous pleurisy (5 men, 5 women, age 64.3 ± 4.4 years) by enzyme-linked immunosorbent assays. Serum levels of healthy individuals served as control. Concentrations of sIL-6R were much higher in serum compared to pleural effusion fluids of tumor patients (25,698 ± 1,993 vs. 9,438 ± 1,407 pg/ml; p < 0.0001). In contrast, IL-6 and IL-8 were found at high concentrations in carcinomatous pleural effusions in comparison to serum (IL-6: 964 ± 176 vs. 10.2 ± 1.3 pg/ml, p < 0.0001; IL-8: 319 ± 85 vs. 9.6 ± 9.6 pg/ml, p < 0.0001). The serum concentrations of IL-6 were not significantly increased in lung cancer patients (10.2 ± 1.3 pg/ml) in comparison to controls (7.3 ± 1.0 pg/ml). IL-8 was detected in the serum of only 1 patient and in low levels in the serum of controls (8.0 ± 1.5 pg/ml; all values are mean ± SEM). We conclude from this study that decreased levels of sIL-6R, but increased levels of IL-6 and IL-8, are found in pleural effusion fluid of patients with lung cancer and carcinomatous pleurisy. The low sIL-6R levels in the presence of high IL-6 levels in pleural effusions and the high sIL-6R levels in the presence of low IL-6 levels in serum may suggest a downregulation of sIL-6R expression or sIL-6R shedding in the presence of excessive amounts of IL-6.

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Section: Discussionmentioning

confidence: 99%

Proinflammatory Cytokine Levels in Patients with Lung Cancer and Carcinomatous Pleurisy

et al. 1998

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“…YM98 and JT99 were established from malignant pleural effusion due to lung adenocarcinoma in our laboratory as described previously. 26) Patients. Sixty-five patients with pleural effusion were examined in Tokushima University Hospital after having obtained informed consent, as described previously.…”

Section: )mentioning

confidence: 99%

Soluble Fas in malignant pleural effusion and its expression in lung cancer cells

et al. 2003

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“…High level production of cytokines was reported in some malignant cell lines such as glioblastoma of the brain (21), multiple myeloma (8), uterine cervical cancer (10), renal cell carcinoma (13), bladder carcinoma (14), and lung cancer (15). Woodworth et al showed high level cytokine secretion from normal cervical keratinocytes and reduced productivity of cytokines in the HPV-immortalized cell lines and cervical cancer cell lines in vitro (25).…”

Section: Discussionmentioning

confidence: 99%

A novel human glassy-cell carcinoma cell line producing IL-6 and IL-8 from uterine cervix

Oki

Nishida

Tsunoda

et al. 1998

In Vitro Cell.Dev.Biol.-Animal

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A novel human cell line, TOM-2, was established from a rare uterine cervical cancer, glassy cell carcinoma (GCC). TOM-2 is the second established GCC cell line so far reported. The cells were intermediately or poorly differentiated with dysplastic nuclei and polygonal shape and secreted two tumor markers and cytokines, i.e., CA-125 and SCC, interleukin (1L)-1alpha, -6, and -8, and TNF-alpha. Growth of TOM-2 was so strongly dependent on population density that it was not possible to determine the plating efficiency. In mass culture, the following characteristics were observed: doubling time, 83 h; mode of chromosome number, 79; human papillomavirus type 18 DNA, detectable; tumorigenicity, easily transplantable into subcutis of nude mice; chemosensitivity in vitro, considerably sensitive to Cisplatin and 5-FU but not to 9 other antineoplastic agents. This novel cell line will be useful for developing new therapeutic strategies for the rare cancer, GCC.

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Spontaneous Production of lnterleukin-8 by Human Lung Cancer Cells and Its Augmentation by Tumor Necrosis Factor Alpha and lnterleukin-1 at Protein and mRNA Levels

Cited by 22 publications

References 15 publications

Proinflammatory Cytokine Levels in Patients with Lung Cancer and Carcinomatous Pleurisy

Proinflammatory Cytokine Levels in Patients with Lung Cancer and Carcinomatous Pleurisy

Soluble Fas in malignant pleural effusion and its expression in lung cancer cells

A novel human glassy-cell carcinoma cell line producing IL-6 and IL-8 from uterine cervix

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