Spontaneous protoplast formation in Methanobacterium bryantii

Jarrell, Ken F.; Colvin, J. Ross; Sprott, G. Dennis

doi:10.1128/jb.149.1.346-353.1982

Cited by 68 publications

(18 citation statements)

References 29 publications

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“…In addition to these known activities, nickel may perform other functions. Methanobacterium bryantii was found to undergo lysis in nickeldepleted media lacking ammonia (98). Thus, nickel may play a role in cell wall biosynthesis or stabilization in this microorganism.…”

Section: Ch3-commentioning

confidence: 99%

Nickel utilization by microorganisms.

Hausinger¹

1987

Microbiological Reviews

160

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Section: Ch3-commentioning

confidence: 99%

Nickel utilization by microorganisms.

Hausinger¹

1987

Microbiological Reviews

160

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“…We describe relatively mild and rapid conditions for obtaining protoplasts of M. voltae, and we demonstrate the regeneration of protoplasts at high efficiencies on agar media. Although protoplast and spheroplast formation in other methanogens has been reported (4,6,9,11,15,24), to our knowledge the current work demonstrates the first successful regeneration of such cell forms in methanogens.…”

Section: Discussionmentioning

confidence: 67%

Formation and Regeneration of Methanococcus voltae Protoplasts

Patel

Choquet

Nash

et al. 1993

Appl Environ Microbiol

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Methanococcus voltae cells were converted into protoplasts by suspension in anaerobic 0.1 M Tris-HCl buffer containing 0.4 M sucrose and 0.05 M NaCl as osmoprotectants. Protoplast formation was monitored microscopically by observing the conversion of the typical irregularly shaped (uneven peripheries) coccoid whole cells to rounded forms with smooth peripheries. Although the procedure resulted in about 50%Yo lysis of the initial number of cells, the remainder were converted to the rounded form. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electron microscopy of negatively stained cell preparations indicated that the treatment removed the wall layer from whole cells to yield protoplasts. Protoplast regeneration was evaluated by using optimized plating conditions and an anaerobic microplating technique. Between 50 and 63% of the initial number of protoplasts regenerated as colonies on agar medium (35°C, 7 days). The colony and cell morphologies of the regenerated protoplasts were indistinguishable from those of whole cells plated under identical conditions.

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“…This was also supported by our experiment. Methanosaeta – an acetoclastic methanogen, which was abundant in the granules on Day 0 – was not detected in the emerging granules, whilst Methanobacterium – autotrophic, H 2 -using methanogens (24–28) also capable of formate reduction (29) – were dominant and likely feeding on increased dissolved hydrogen resulting from the accumulating VFA (30). Propionibacteriaceae – a family of heterotrophic glucose fermenters, producing propionate and acetate as primary products (31) – were also abundant in new granules, likely as VFA-producing acetogens.…”

Section: Discussionmentioning

confidence: 99%

De NovoGrowth of Methanogenic Granules Indicates a Biofilm Life-Cycle with Complex Ecology

Trego

Galvin

Sweeney

et al. 2019

Preprint

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395 words (249 in 'Abstract' + 146 in 'Importance') 8 Text: 3,980 words (excl. references, table footnotes, figure legends) 9Abstract. Methanogenic sludge granules are densely packed, small (diameter, 1 approx. 0.5-2.0 mm) spherical biofilms found in anaerobic digesters used to treat 2 industrial wastewaters, where they underpin efficient organic waste conversion and 3 biogas production. A single digester contains millions of individual granules, each of 4 which is a highly-organised biofilm comprised of a complex consortium of likely 5 billions of cells from across thousands of speciesbut not all granules are identical. 6Whilst each granule theoretically houses representative microorganisms from all of 7 the trophic groups implicated in the successive and interdependent reactions of the 8 anaerobic digestion process, parallel granules function side-by-side in digesters to 9 provide a 'meta-organism' of sorts. Granules from a full-scale bioreactor were size-10 separated into small, medium and large granules. Laboratory-scale bioreactors were 11 operated using only small (0.6-1 mm), medium (1-1.4 mm) or large (1.4-1.8 mm) 12 granules, or unfractionated (naturally distributed) sludge. After >50 days of 13 operation, the granule size distribution in each of the small, medium and large 14 bioreactor types had diversified beyondto both bigger and smaller thanthe size 15 fraction used for inoculation. 'New' granules were analysed by studying community 16 structure based on high-throughput 16S rRNA gene sequencing. Methanobacterium, 17Aminobacterium, Propionibacteriaceae and Desulfovibrio represented the majority of 18 the community in new granules. H2-using, and not acetoclastic, methanogens 19 appeared more important, and were associated with abundant syntrophic bacteria. 20Multivariate integration analyses identified distinct discriminant taxa responsible for 21 shaping the microbial communities in different-sized granules, and along with alpha 22 diversity data, indicated a possible biofilm life cycle. 23Importance. Methanogenic granules are spherical biofilms found in the built 24 environment, where despite their importance for anaerobic digestion of wastewater 25

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Spontaneous protoplast formation in Methanobacterium bryantii

Cited by 68 publications

References 29 publications

Nickel utilization by microorganisms.

Nickel utilization by microorganisms.

Formation and Regeneration of Methanococcus voltae Protoplasts

De NovoGrowth of Methanogenic Granules Indicates a Biofilm Life-Cycle with Complex Ecology

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