Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (<i>Chinchilla lanigera</i>)

Kuchta-Gładysz, Marta; Wójcik, Ewa; Szeleszczuk, Olga; Niedbała, Piotr; Tyblewska, Kaja

doi:10.4141/cjas-2015-073

Cited by 4 publications

(2 citation statements)

References 54 publications

(70 reference statements)

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“…They observed an increase in the frequency of damage in older individuals compared to young horses. Age is the factor that affects the level of damage generated; the older the animal, the more damage we observe [15,32,44,45]. This dependency was also noted in the present study for each assay used.…”

Section: Plos Onesupporting

confidence: 85%

Assessment of chromosome stability in boars

Wójcik

Sokół

2020

PLoS ONE

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Chromosome instability adversely affects animal fertility and reproduction. Analysis of instability can be a valuable diagnostic tool. Helpful tests for assessment of instabilities include the sister chromatid exchange assay, identification of fragile sites, the bleomycin assay and the comet assay. These techniques can be used to assess and compare the chromosome stability of individual breeds of animals. The aim of the study was to assess chromosome stability in boars: Duroc, Duroc x Pietrain and Pietrain x Duroc crossbreds, Polish Large White, and the Neckar, P76 and PIC lines. The study assessed the chromosome stability of boars. The distribution of instabilities in individual breeds was varied. The average frequency of chromatid exchange was 4.8 ± 1.5, while that of fragile sites was 3.9 ± 1.4. The mean level of DNA damage (% tail DNA) was 9.4 ± 8.3, while in the bleomycin assay b/c and %AM were 0.6 ± 0.7 and 44.4 ± 4.1. A higher rate of instability was found in older individuals than in younger ones. The cytogenetic assays used to identify various forms of chromosome instability can be used to evaluate boars intended for breeding.

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Section: Plos Onesupporting

confidence: 85%

Assessment of chromosome stability in boars

Wójcik

Sokół

2020

PLoS ONE

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“…According to many researchers, exchanges take place less frequently in the terminal part of chromosomes [32,53]. On the other hand, Kuchta-Gładysz et al [50,54] have reported a very high level of damage in the distal part of the chromosome. Damage in this part of the chromosome is caused by oxidative stress, which results in single-strand DNA breakage in the telomeres.…”

Section: Discussionmentioning

confidence: 99%

Assessment of genome stability in various breeds of cattle

Wójcik

Szostek

2019

PLoS ONE

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Chromosomal instability is a type of genome instability involving changes in genetic information at the chromosomal level. The basic tests used to identify this form of instability are sister chromatid exchange (SCE) tests and identification of fragile sites (FS). SCE is the process by which sister chromatids become fragmented as a result of DNA strand breakage and reassembly, followed by exchange of these fragments. FS can be observed in the form of breaks, gaps or constrictions on chromosomes, which often result from multiple nucleotide repeats in DNA that are difficult to replicate. The research material was the peripheral blood of ten breeds of cattle raised in Poland, including four native breeds covered by a genetic resources conservation programme, i.e. Polish Red, Polish Red-and-White, White-Backed, and Polish Black-and-White, as well as Polish Holstein-Friesian, Simmental, Montbéliarde, Jersey, Limousine and Danish Red. Two tests were performed on chromosomes obtained from in vitro cultures: SCE and FS. The average frequency of SCE was 5.08 ± 1.31, while the incidence of FS was 3.45 ± 0.94. Differences in the incidence of SCE and FS were observed between breeds. The least damage was observed in the Polish Red and White-Backed breeds, and the most in Polish Holstein-Friesians. The most damage was observed in the interstitial part of the chromosomes. Age was shown to significantly affect the incidence of SCE and FS. Younger cows showed less damage than older ones (SCE: 4.84 ± 1.25; 5.34 ± 1.24; FS: 3.10 ± 0.88, 3.80 ± 0.92).

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Similar Sister Chromatid Arrangement in Mono- and Holocentric Plant Chromosomes

Schubert

Zelkowski²,

Klemme³

et al. 2016

Cytogenet Genome Res

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Due to the X-shape formation at somatic metaphase, the arrangement of the sister chromatids is obvious in monocentric chromosomes. In contrast, the sister chromatids of holocentric chromosomes cannot be distinguished even at mitotic metaphase. To clarify their organization, we differentially labelled the sister chromatids of holocentric Luzula and monocentric rye chromosomes by incorporating the base analogue EdU during replication. Using super-resolution structured illumination microscopy (SIM) and 3D rendering, we found that holocentric sister chromatids attach to each other at their contact surfaces similar to those of monocentrics in prometaphase. We found that sister chromatid exchanges (SCEs) are distributed homogeneously along the whole holocentric chromosomes of Luzula, and that their occurrence is increased compared to monocentric rye chromosomes. The SCE frequency of supernumerary B chromosomes, present additionally to the essential A chromosome complement of rye, does not differ from that of A chromosomes. Based on these results, models of the sister chromatid arrangement in mono- and holocentric plant chromosomes are presented.

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Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)

Cited by 4 publications

References 54 publications

Assessment of chromosome stability in boars

Assessment of chromosome stability in boars

Assessment of genome stability in various breeds of cattle

Similar Sister Chromatid Arrangement in Mono- and Holocentric Plant Chromosomes

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