Spontaneous uptake of biologically active recombinant DNA by mammalian cells via a selected DNA segment

Gene delivery with biocompatible cationic polymer: Pharmacogenomic analysis on cell bioactivity

“…The uptake amount of reporter gene into the HuH-7 cells by the P[Asp(DET)] or LPEI polyplex was determined by a real-time PCR in terms of gene copies per cell from the total DNA samples [16][17][18]. As seen in Fig.…”

Gene Transfer and Cauliflower Mosaic Virus Promoter 35S Activity in Mammalian Cells

“…[30,31] In light of current traceability regulations, it would be desirable to develop novel biomarkers and sensitive molecular techniques to monitor foods and the food chain for the possible presence of transgenic elements introduced with fodder, birdseed, or feedstuffs. These traceability approaches may also contribute to surveillance and prevention of unknown long-term effects on humans and environment.…”

Bioinformatics and experimental derivation of an efficient hybrid 3′ untranslated region and use in expression active linear DNA with minimum poly(A) region

“…For example, the intensity of GFP expressed from the expression active DNA in HEK293 was 64 million pixels compared to 120 million pixels of the modified vector, but still substantially higher than the unmodified vector (35 million pixels). The uptake efficiency of linear DNA can possibly be enhanced by the use of cationic lipid as charge neutralizer (von Groll et al, 2006) or by further insertion of experimentally validated uptake-enhancing 100 bp sequence (Lehmann and Sczakiel, 2005), but these have not been tested in our hands. The linear constructs were able to continue expressing GFP in Huh7 cells by the end of six days in culture in comparison to the expression vectors (data not shown).…”