Spontaneously Arising Canine Glioma as a Potential Model for Human Glioma

Herranz, Cristina; Fernández, F.; Martín‐Ibáñez, Raquel; Blasco, Ester; Crespo, Empar; Fuente, Cristian de la; Añor, S.; Rabanal, Rosa M.; Canals, Josep M.; Pumarola, M.

doi:10.1016/j.jcpa.2015.12.001

Cited by 41 publications

(39 citation statements)

References 30 publications

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“…Cortical and caudate nucleus samples from the same dogs were processed as negative controls. Conditions for primary tissue culture were adapted from Stoica et al (2009) and were carried out following the same protocols as published for samples of canine gliomas (Herranz et al, ). Samples were cut into small pieces and subsequently dissociated mechanically in control medium.…”

Section: Methodsmentioning

confidence: 99%

“…The culture medium was then changed to DM2, which consisted of CM supplemented with 2% B27 and 2% fetal bovine serum (FBS; Life Technologies), for the following 3 days. Under these conditions adult NSC were able to differentiate spontaneously toward the three neural lineages: neurons, astrocytes, and oligodendrocytes, as described previously (Herranz et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…To date there is only a single ultrastructural description of the V‐SVZ in dogs in which the presence of a subependymal cell plate and ultrastructural features are described (Blakemore & Jolly, ). Furthermore, capacity to form neurospheres in vitro partially composed of a neuroepithelial cell subpopulation has been described in dogs through isolation of grafts from the V‐SVZ (Herranz et al, ; Lim, Koh, Olby, Piedrahita, & Mariani, ; Walton et al, ), olfactory bulb (Walton & Wolfe, ), and hippocampus (Lowe et al, ) suggesting ability to produce new neural cells during the early postnatal life in canine species.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the canine rostral ventricular‐subventricular zone: Morphological, immunohistochemical, ultrastructural, and neurosphere assay studies

Fernández-Flores

García‐Verdugo

Martín‐Ibáñez

et al. 2017

J of Comparative Neurology

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The mammalian ventricular-subventricular zone (V-SVZ) presents the highest neurogenic potential in the brain of the adult individual. In rodents, it is mainly composed of chains of neuroblasts. In humans, it is organized in layers where neuroblasts do not form chains. The aim of this study is to describe the cytoarchitecture of canine V-SVZ (cV-SVZ), to assess its neurogenic potential, and to compare our results with those previously described in other species. We have studied by histology, immunohistochemistry (IHC), electron microscopy and neurosphere assay the morphology, cytoarchitecture and neurogenic potential of cV-SVZ. Age groups of animals were performed. Histological and ultrastructural studies indicated that the cV-SVZ is organized in layers as in humans, but including migratory chains as in rodents. Neural progenitors were organized in niches in the subependymal area and a decline in their number was observed with age. Adult-young dogs contained migratory cells capable to expand and differentiate in vitro according with previous results obtained in rodents, primates, humans, pigs, and dogs. Some adult animals presented perivascular niches outside the V-SVZ. Our observations evidence a great similarity between canine and human V-SVZ indicating that the dog may be better representative of neurogenic events in humans, compared with rodents. Accordingly with our results, we conclude that dogs are a valuable animal model of adult neurogenesis in comparative and preclinical studies.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of the canine rostral ventricular‐subventricular zone: Morphological, immunohistochemical, ultrastructural, and neurosphere assay studies

Fernández-Flores

García‐Verdugo

Martín‐Ibáñez

et al. 2017

J of Comparative Neurology

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“…206 Clinical studies will be accompanied by laboratory correlative studies that seek to describe, characterize, and understand the cellular and molecular mechanisms that determine the antitumor response (or lack of response) in dogs with spontaneous tumors. Specifically, the spontaneous tumor types that have been deliberately targeted as comparative for immunotherapeutic development include lymphoma, 92,98,207,208 osteosarcoma, 95,97,[209][210][211][212] mammary gland cancer, 106,107,213,214 brain cancer, 199,[215][216][217] melanoma, [218][219][220] and transitional cell carcinoma 221,222 (Table 3).…”

Section: Ongoing and Future Translational Opportunitiesmentioning

confidence: 99%

Of Mice, Dogs, Pigs, and Men: Choosing the Appropriate Model for Immuno-Oncology Research

et al. 2018

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The immune system plays dual roles in response to cancer. The host immune system protects against tumor formation via immunosurveillance; however, recognition of the tumor by immune cells also induces sculpting mechanisms leading to a Darwinian selection of tumor cell variants with reduced immunogenicity. Cancer immunoediting is the concept used to describe the complex interplay between tumor cells and the immune system. This concept, commonly referred to as the three E’s, is encompassed by 3 distinct phases of elimination, equilibrium, and escape. Despite impressive results in the clinic, cancer immunotherapy still has room for improvement as many patients remain unresponsive to therapy. Moreover, many of the preclinical results obtained in the widely used mouse models of cancer are lost in translation to human patients. To improve the success rate of immuno-oncology research and preclinical testing of immune-based anticancer therapies, using alternative animal models more closely related to humans is a promising approach. Here, we describe 2 of the major alternative model systems: canine (spontaneous) and porcine (experimental) cancer models. Although dogs display a high rate of spontaneous tumor formation, an increased number of genetically modified porcine models exist. We suggest that the optimal immuno-oncology model may depend on the stage of cancer immunoediting in question. In particular, the spontaneous canine tumor models provide a unique platform for evaluating therapies aimed at the escape phase of cancer, while genetically engineered swine allow for elucidation of tumor-immune cell interactions especially during the phases of elimination and equilibrium.

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“…Canine patients also suffer from malignant gliomas, with tumors that are largely similar in histopathological appearance, clinical progression, and some key molecular features (2)(3)(4)(5). Dogs are increasingly of interest in comparative glioma research as potential animal models, owing to the fact that gliomas develop in these patients in the context of a large, complex brain, an intact immune system, increased genomic similarity to humans as compared to rodent models, and similar environmental exposures (6)(7)(8)(9)(10)(11). Because the pathology classification of canine gliomas has recently undergone a revision as part of the efforts of the Comparative Brain Tumor Consortium in the Comparative Oncology Program of the National Institute of Health's National Cancer Institute (6,12), in discussions here, the canine tumors will be referred to by the broader appellation "high-grade gliomas, " while the human tumors will retain their more specific designations when available and appropriate.…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of miRNAs in Hypoxia (“HypoxamiRs”) in Three Canine High-Grade Glioma Cell Lines

et al. 2020

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Dogs with spontaneous high-grade gliomas increasingly are being proposed as useful large animal pre-clinical models for the human disease. Hypoxia is a critical microenvironmental condition that is common in both canine and human high-grade gliomas and drives increased angiogenesis, chemo-and radioresistance, and acquisition of a stem-like phenotype. Some of this effect is mediated by the hypoxia-induced expression of microRNAs, small (∼22 nucleotides long), non-coding RNAs that can modulate gene expression through interference with mRNA translation. Using an in vitro model with three canine high-grade glioma cell lines (J3T, SDT3G, and G06A) exposed to 72 h of 1.5% oxygen vs. standard 20% oxygen, we examined the global "hypoxamiR" profile using small RNA-Seq and performed pathway analysis for targeted genes using both Panther and NetworkAnalyst. Important pathways include many that are well-established as being important in glioma biology, general cancer biology, hypoxia, angiogenesis, immunology, and stem-ness, among others. This work provides the first examination of the effect of hypoxia on miRNA expression in the context of canine glioma, and highlights important similarities with the human disease.

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Spontaneously Arising Canine Glioma as a Potential Model for Human Glioma

Cited by 41 publications

References 30 publications

Characterization of the canine rostral ventricular‐subventricular zone: Morphological, immunohistochemical, ultrastructural, and neurosphere assay studies

Characterization of the canine rostral ventricular‐subventricular zone: Morphological, immunohistochemical, ultrastructural, and neurosphere assay studies

Of Mice, Dogs, Pigs, and Men: Choosing the Appropriate Model for Immuno-Oncology Research

Differential Expression of miRNAs in Hypoxia (“HypoxamiRs”) in Three Canine High-Grade Glioma Cell Lines

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