Spontaneously Blinking Rhodamine Dyes for Single‐Molecule Localization Microscopy

Chi, Weijie; Tan, Davin; Qiao, Qinglong; Xu, Zhaochao; Liu, Xiaogang

doi:10.1002/anie.202306061

Cited by 18 publications

(14 citation statements)

References 43 publications

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“…4c). Incorporating heteroatoms into the rhodamine framework elicits a redshift in λ abs / λ em , 31–33 evidencing through the decreased Δ E H–L (Fig. 4d).…”

Section: Resultsmentioning

confidence: 92%

Photoinduced electron transfer endows fluorogenicity in tetrazine-based near-infrared labels

Shen,

Li,

Liu

2024

Mater. Chem. Front.

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“…4c). Incorporating heteroatoms into the rhodamine framework elicits a redshift in λ abs / λ em , 31–33 evidencing through the decreased Δ E H–L (Fig. 4d).…”

Section: Resultsmentioning

confidence: 92%

Photoinduced electron transfer endows fluorogenicity in tetrazine-based near-infrared labels

Shen,

Li,

Liu

2024

Mater. Chem. Front.

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“…As this review focuses on heterogeneous catalysis, we will primarily discuss probes that undergo irreversible chemical reactions that activate them into their fluorescent state. Fluorogenic probes that reversibly interconvert between their nonfluorescent and fluorescent states through changes in pH and/or temperature − or an applied electrical bias , are used in other SMF imaging techniques. Once activated, individual fluorescent probes can be detected using fluorescence microscopy, allowing single-turnover counting of the reaction events that occur on the catalyst surface.…”

Section: A Comparison Of Techniques For In Situ Imaging Of Heterogene...mentioning

confidence: 99%

Getting the Most Out of Fluorogenic Probes: Challenges and Opportunities in Using Single-Molecule Fluorescence to Image Electro- and Photocatalysis

Shen,

Rackers,

Sadtler

2023

Chemical & Biomedical Imaging

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Single-molecule fluorescence microscopy enables the direct observation of individual reaction events at the surface of a catalyst. It has become a powerful tool to image in real time both intraand interparticle heterogeneity among different nanoscale catalyst particles. Single-molecule fluorescence microscopy of heterogeneous catalysts relies on the detection of chemically activated fluorogenic probes that are converted from a nonfluorescent state into a highly fluorescent state through a reaction mediated at the catalyst surface. This review article describes challenges and opportunities in using such fluorogenic probes as proxies to develop structure−activity relationships in nanoscale electrocatalysts and photocatalysts. We compare single-molecule fluorescence microscopy to other microscopies for imaging catalysis in situ to highlight the distinct advantages and limitations of this technique. We describe correlative imaging between super-resolution activity maps obtained from multiple fluorogenic probes to understand the chemical origins behind spatial variations in activity that are frequently observed for nanoscale catalysts. Fluorogenic probes, originally developed for biological imaging, are introduced that can detect products such as carbon monoxide, nitrite, and ammonia, which are generated by electro-and photocatalysts for fuel production and environmental remediation. We conclude by describing how single-molecule imaging can provide mechanistic insights for a broader scope of catalytic systems, such as single-atom catalysts.

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“…Rh-Gly, [36] and HMSiR [15] as extensively reviewed. [6,37,38] Rather than tuning the equilibrium of rhodamines, Julian et al modified the labeling methods for various SRMs. [39] Usually, fluorogenic rhodamines are conjugated with chloroalkanes (the ligand for HaloTag) to achieve covalent labeling, resulting in high brightness and image contrast.…”

Section: Fluorogenic and Cell-permeable Rhodamines For Live-cell Prot...mentioning

confidence: 99%

Fluorogenic and Cell‐Permeable Rhodamine Dyes for High‐Contrast Live‐Cell Protein Labeling in Bioimaging and Biosensing

Bao

et al. 2023

Angew Chem Int Ed

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The advancement of fluorescence microscopy techniques has opened up new opportunities for visualizing proteins and unraveling their functions in living biological systems. Small‐molecule organic dyes, which possess exceptional photophysical properties, small size, and high photostability, serve as powerful fluorescent reporters in protein imaging. However, achieving high‐contrast live‐cell labeling of target proteins with conventional organic dyes remains a considerable challenge in bioimaging and biosensing due to their inadequate cell permeability and high background signal. Over the past decade, a novel generation of fluorogenic and cell‐permeable dyes has been developed, which have substantially improved live‐cell protein labeling by fine‐tuning the reversible equilibrium between a cell‐permeable, nonfluorescent spirocyclic state (unbound) and a fluorescent zwitterion (protein‐bound) of rhodamines. In this review, we present the mechanism and design strategies of these fluorogenic and cell‐permeable rhodamines, as well as their applications in bioimaging and biosensing.

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Spontaneously Blinking Rhodamine Dyes for Single‐Molecule Localization Microscopy

Cited by 18 publications

References 43 publications

Photoinduced electron transfer endows fluorogenicity in tetrazine-based near-infrared labels

Photoinduced electron transfer endows fluorogenicity in tetrazine-based near-infrared labels

Getting the Most Out of Fluorogenic Probes: Challenges and Opportunities in Using Single-Molecule Fluorescence to Image Electro- and Photocatalysis

Fluorogenic and Cell‐Permeable Rhodamine Dyes for High‐Contrast Live‐Cell Protein Labeling in Bioimaging and Biosensing

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