Spontaneously Produced Lysogenic Phages Are an Important Component of the Soybean
            <i>Bradyrhizobium</i>
            Mobilome

Joglekar, Prasanna; Ferrell, Barbra D.; Jarvis, Tessa; Haramoto, Kona; Place, Nicole M.; Dums, Jacob T.; Polson, Shawn W.; Wommack, K. Eric; Fuhrmann, Jeffry J.

doi:10.1128/mbio.00295-23

Cited by 4 publications

(2 citation statements)

References 94 publications

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“…Interspecific competition between maizesoybean intercropping leads to more efficient utilization of applied N (Zhang et al, 2023). Adding Bradyrhizobium with these three micronutrients zinc, iron, and molybdenum have resulted in a remarkable yield response in soybean as reported earlier by Tiwari et al (2018) and Joglekar et al (2023). Furthermore, Mo can influence the interaction between maize and soybean in intercropping systems, potentially impacting overall crop productivity and quality (Abendroth et al, 2017;Oliveira et al, 2022).…”

Section: Introductionmentioning

confidence: 78%

Assessment of molybdenum application on soybean physiological characteristics in maize-soybean intercropping

Jamali,

Ali,

Qasim

et al. 2023

Front. Plant Sci.

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Soybean is a leguminous crop known for its efficient nitrogen utilization and ease of cultivation. However, its intercropping with maize may lead to severe reduction in its growth and yield due to shading effect of maize. This issue can be resolved by the appropriate application of essential plant nutrient such as molybdenum (Mo). Aim of this study was to assess the effect of Mo application on the morphological and physiological characteristics of soybean intercropped with maize. A two-year field experiment was conducted for this purpose, and Mo was applied in the form of sodium molybdate (Na2MoO4), and four different levels were maintained i.e., 0, 60, 120 and 180 g ha-1. Soybean exhibited varying responses to different levels of molybdenum (Mo) application. Notably, in both sole and intercropped cropping systems, the application of Mo at a rate of 120 g ha-1 demonstrated the highest level of promise compared to other application levels. However, most significant outcomes were pragmatic in soybean-maize intercropping, as application of Mo @ 120 g ha-1 significantly improved soybean growth and yield attributes, including leaf area index (LAI; 434 and 441%), total plant biomass (430 and 461%), transpiration rate (15 and 18%), stomatal conductance (9 and 11%), and yield (15 and 20%) during year 2020 and 2021 respectively, as compared to control treatment. Similarly, Mo @ 120 g ha-1 application resulted in highest total grain yield (626.0 and 725.3 kg ha-1) during 2020 and 2021 respectively, which exceeded the grain yields of other Mo levels under intercropping. Moreover, under Mo application level (120 g ha-1), grain NPK and Mo contents during years 2020 and 2021 were found to be 1.15, 0.22, 0.83 and 68.94 mg kg-1, and 1.27, 0.25, 0.90 and 72.18 mg kg−1 under intercropping system increased the value as compared to control treatment. Findings of current study highlighted the significance of Mo in enhancing soybean growth, yield, and nutrient uptake efficiency in maize-soybean intercropping systems.

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Section: Introductionmentioning

confidence: 78%

Assessment of molybdenum application on soybean physiological characteristics in maize-soybean intercropping

Jamali,

Ali,

Qasim

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

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“…In addition, studies have shown that type VI secretion systems ( Bladergroen et al., 2003 ; De Campos et al., 2017 ) as well as the production of phages ( Schwinghamer and Brockwell, 1978 ; Joglekar et al., 2023 ) or bacteriocins ( Hirsch, 1979 ; Triplett and Barta, 1987 ; Oresnik et al., 1999 ) in rhizobia can play an important role in the direct inhibition of bacterial competitors. Among antagonistic molecules produced by rhizobia, diffusible quorum sensing molecules, in particular Acyl Homoserine Lactones (AHLs), can inhibit the growth of other rhizobial strains by activating LuxR-type regulators in the neighbouring strains and inducing genes leading to growth arrest ( Schripsema et al., 1996 ; Wilkinson et al., 2002 ).…”

Section: The Rhizosphere Microbiome: a Hotspot For Microbial Interact...mentioning

confidence: 99%

The role of microbial interactions on rhizobial fitness

Granada Agudelo,

Ruiz,

Capela

et al. 2023

Front. Plant Sci.

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Rhizobia are soil bacteria that can establish a nitrogen-fixing symbiosis with legume plants. As horizontally transmitted symbionts, the life cycle of rhizobia includes a free-living phase in the soil and a plant-associated symbiotic phase. Throughout this life cycle, rhizobia are exposed to a myriad of other microorganisms that interact with them, modulating their fitness and symbiotic performance. In this review, we describe the diversity of interactions between rhizobia and other microorganisms that can occur in the rhizosphere, during the initiation of nodulation, and within nodules. Some of these rhizobia-microbe interactions are indirect, and occur when the presence of some microbes modifies plant physiology in a way that feeds back on rhizobial fitness. We further describe how these interactions can impose significant selective pressures on rhizobia and modify their evolutionary trajectories. More extensive investigations on the eco-evolutionary dynamics of rhizobia in complex biotic environments will likely reveal fascinating new aspects of this well-studied symbiotic interaction and provide critical knowledge for future agronomical applications.

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Biochemical characterisation and production kinetics of high molecular-weight (HMW) putative antibacterial proteins of insect pathogenic Brevibacillus laterosporus isolates

Babar,

Glare,

Hampton

et al. 2024

BMC Microbiol

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Background Bacterial genomes often encode structures similar to phage capsids (encapsulins) and phage tails which can be induced spontaneously or using genotoxic compounds such as mitomycin C. These high molecular-weight (HMW) putative antibacterial proteins (ABPs) are used against the competitive strains under natural environment. Previously, it was unknown whether these HMW putative ABPs originating from the insect pathogenic Gram-positive, spore-forming bacterium Brevibacillus laterosporus (Bl) isolates (1821L, 1951) are spontaneously induced during the growth and pose a detrimental effect on their own survival. Furthermore, no prior work has been undertaken to determine their biochemical characteristics. Results Using a soft agar overlay method with polyethylene glycol precipitation, a narrow spectrum of bioactivity was found from the precipitated lysate of Bl 1951. Electron micrographs of mitomycin C- induced filtrates showed structures similar to phage capsids and contractile tails. Bioactivity assays of cell free supernatants (CFS) extracted during the growth of Bl 1821L and Bl 1951 suggested spontaneous induction of these HMW putative ABPs with an autocidal activity. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of spontaneously induced putative ABPs showed appearance of ~ 30 kDa and ~ 48 kDa bands of varying intensity across all the time intervals during the bacterial growth except in the initial hours. Statistically, spontaneously induced HMW putative ABPs of Bl 1951 exhibited a significant decrease in the number of viable cells of its producer strain after 18 h of growth in liquid. In addition, a significant change in pH and prominent bioactivity of the CFS of this particular time period was noted. Biochemically, the filtered supernatant derived from either Bl 1821L or Bl 1951 maintained bioactivity over a wide range of pH and temperature. Conclusion This study reports the spontaneous induction of HMW putative ABPs (bacteriocins) of Bl 1821L and Bl 1951 isolates during the course of growth with potential autocidal activity which is critically important during production as a potential biopesticide. A narrow spectrum of putative antibacterial activity of Bl 1951 precipitate was found. The stability of HMW putative ABPs of Bl 1821L and Bl 1951 over a wide range of pH and temperature can be useful in expanding the potential of this useful bacterium beyond the insecticidal value.

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Spontaneously Produced Lysogenic Phages Are an Important Component of the Soybean Bradyrhizobium Mobilome

Cited by 4 publications

References 94 publications

Assessment of molybdenum application on soybean physiological characteristics in maize-soybean intercropping

Assessment of molybdenum application on soybean physiological characteristics in maize-soybean intercropping

The role of microbial interactions on rhizobial fitness

Biochemical characterisation and production kinetics of high molecular-weight (HMW) putative antibacterial proteins of insect pathogenic Brevibacillus laterosporus isolates

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