Sporadic isolation of<i>Tetratrichomonas</i>species from the cattle urogenital tract

Bailey, Nicholas P; Velo-Rego, Elena; Hirt, Robert P.

doi:10.1017/s003118202100086x

Cited by 4 publications

(3 citation statements)

References 45 publications

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“…A phylogenetic tree was constructed by using the neighborjoining (NJ) method described previously [16]. Genetic distances were calculated in MEGA 7 software using the Kimura parameter-2 model [17], and the robustness of the findings was evaluated with 1000 bootstrap replicates [18].…”

Section: Sequencing and Phylogenetic Analysismentioning

confidence: 99%

Molecular Identification and Survey of Trichomonad Species in Pigs in Shanxi Province, North China

Wang,

Fan,

Wang

et al. 2024

Veterinary Sciences

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Several trichomonad species have already been identified in pigs, and their pathogenic potential may not be ruled out. To date, however, no information is available regarding the prevalence of trichomonads in pigs in Shanxi Province, North China. In the present study, a total of 362 fecal samples collected from pigs in three representative counties (Qi, Jishan, and Shanyin) in this province were examined for Tetratrichomonas buttreyi, Tritrichomonas foetus, and Pentatrichomonas hominis using a nested polymerase chain reaction (PCR) with primers targeting the small subunit ribosomal RNA (SSU rRNA) gene. The overall prevalence of T. buttreyi was 49.72%, and region and age were found to be significantly associated with T. buttreyi infection, respectively. Only one pig fecal sample from Qi County was found to be positive for T. foetus, and all samples were negative for P. hominis. Molecular evolutionary analysis revealed that some T. buttreyi isolates showed complete genetic identity with those reported previously, and some T. buttreyi isolates and one T. foetus isolate showed minor allelic variations compared with those reported previously. This is the report of the molecular epidemiology of T. foetus and T. buttreyi in pigs in Shanxi Province, North China. These findings not only enrich the knowledge on the distribution of these trichomonad species in pigs in China but also provide baseline information for planning future research and control strategies.

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Section: Sequencing and Phylogenetic Analysismentioning

confidence: 99%

Molecular Identification and Survey of Trichomonad Species in Pigs in Shanxi Province, North China

Wang,

Fan,

Wang

et al. 2024

Veterinary Sciences

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“…In this context, the presence of these trichomonads in samples collected for T. foetus testing thus represents a potential problem during routine screening for bovine tritrichomonosis. Although in different countries, artificial insemination and control programs have greatly reduced the apparition of trichomonads of gastrointestinal origin and T. foetus isolated from the bull’s preputial cavity ( 21 , 22 ).…”

Section: Introductionmentioning

confidence: 99%

Prolonged survival of venereal Tritrichomonas foetus parasite in the gastrointestinal tract, bovine fecal extract, and water

Martínez,

Iriarte,

Salas

et al. 2023

Microbiol Spectr

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Bovine tritrichomonosis is a venereal disease that causes economic losses around the world. Over the last 100 years, the life cycle of the protozoan Tritrichomonas foetus in bovines has only considered venereal transmission, almost exclusively through natural mating from an infected animal to a healthy animal. Here, we provide the first direct evidence that T. foetus can survive the passage through the gastrointestinal tract in bovines. We demonstrated that the parasite can be discharged by feces in orally infected animals and contaminate the cow’s reproductive tract. We showed that the parasite is capable of surviving at pH conditions usually found along the bovine gastrointestinal tract, and our findings suggest that the parasites could survive in bovine feces and water for several days, probably through the formation of resistant pseudocysts or cyst-like structures. Taken together, our results demonstrate that T. foetus is more resistant to adverse external conditions than previously considered, which could be relevant for the life cycle of this parasite in bovines. IMPORTANCE Nowadays, the routine herd diagnosis is usually performed exclusively on bulls, as they remain permanently infected, and prevention and control of Tritrichomonas foetus transmission are based on identifying infected animals and culling practices. The existence of other forms of transmission and the possible role of pseudocysts or cyst-like structures as resistant forms requires rethinking the current management and control of this parasitic disease in the future in some livestock regions of the world.

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“…In contrast, there exists robust evidence demonstrating the ability of P. hominis to colonize the preputial cavity of bulls in North America (Hayes et al, 2003;Walker et al, 2003;Dufernez et al, 2007). In addition to confirming the ability of the protozoan to inhabit non-gastrointestinal body sites, the presence of P. hominis in the bovine urogenital tract, represents a problem for the accurate diagnosis of Tritrichomonas foetus, an economically important venereal trichomonad parasite of cattle that causes reproductive issues, including spontaneous abortion, pyometra and infertility (Bailey et al, 2021). In a subsequent study (Corbeil et al, 2008), the isolation of two non-T. foetus trichomonads, (identified as P. hominis based on the results of PCR and immunofluorescence assays) was recorded from the vaginas of breeding cows in the United States of America (USA).…”

Section: Introductionmentioning

confidence: 99%

Identification of Pentatrichomonas hominis in preputial washes of bulls in Brazil

Silva

Ribeiro

Jesus

et al. 2022

Rev. Bras. Parasitol. Vet.

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The parabasalid Pentatrichomonas hominis is generally considered to represent a symbiotic component of the gastrointestinal microbiota in a wide variety of vertebrate hosts including humans. Nevertheless, a limited number of studies have raised the possibility that it may act as a pathogen of humans, dogs, and pigs and that some human infections may have a zoonotic origin. Data from North America revealed an association between P. hominis and the bovine urogenital tract, principally in bulls and rarely in cows. The importance of this observation is linked to possible interference in the accurate diagnosis of the economically important venereal pathogen Tritrichomonas foetus. The current study employed culture-based and molecular methods to examine the preputial cavities of four breeding bulls, raised in open pasture in southeastern Brazil, for the presence of trichomonads. Motile protozoa were isolated from three of the bulls and were definitively identified as P. hominis based on nucleotide sequencing of polymerase chain reaction (PCR) amplicons derived from the ribosomal RNA operon (ITS1-5.8S rDNA-ITS2) of the parasite. The potential implications of these findings for bovine and human health are discussed.

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Sporadic isolation ofTetratrichomonasspecies from the cattle urogenital tract

Abstract: Abstract

Cited by 4 publications

References 45 publications

Molecular Identification and Survey of Trichomonad Species in Pigs in Shanxi Province, North China

Molecular Identification and Survey of Trichomonad Species in Pigs in Shanxi Province, North China

Prolonged survival of venereal Tritrichomonas foetus parasite in the gastrointestinal tract, bovine fecal extract, and water

Identification of Pentatrichomonas hominis in preputial washes of bulls in Brazil

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