Spore coat protein synthesis during development of Dicytostelium discoideum requires a low-molecular-weight inducer and continued multicellularity

Wilkinson, David G.; Wilson, J. Jeffrey; Hames, B.D.

doi:10.1016/0012-1606(85)90373-2

Cited by 31 publications

(8 citation statements)

References 25 publications

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“…The transition to the tipped mound stage also involves the synthesis of many other spore specific proteins and the appearance of spore-associated enzyme activities and organelles such as the prespore vacuoles (Hohl and Hamamoto 1969;Gregg and Badman 1970;Krefft et al 1984;Morrissey et al 1984;Miiller and Hohl1973;Cardelli et al 1985Cardelli et al ,1990Lam and Siu 1981;Devine et al 1983;Wilkinson et al 1985;West and Erdos 1990). There is also some evidence that at this time the a-mannosidase (synthesized after starvation) may be transferred to the prespore vesicle, and then later secreted along with spore wall components after culmination (Lenhard et al 1989).…”

Section: Prespore Cells Prestalk Cells and Tip Formationmentioning

confidence: 99%

Patterning of development in Dictyostelium discoideum: factors regulating growth, differentiation, spore dormancy, and germination

Cotter

Sands²,

Virdy³

et al. 1992

Biochem. Cell Biol.

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Cellular communication dictates all stages of growth and development in the cellular slime molds. Dictyostelium discoideum utilizes a number of signal molecules for cell-to-cell communication, including growth and density factors, cAMP, ammonia, differentiation-inducing factor, discadenine, and spore autoactivator. A source and sink model is presented in which the assimilation of ammonia plays a major role in determining cell fate and pattern formation. This model emphasizes a recycling of ammonia by prespore cells, the accumulation of free hydrophilic and neutral amino acids, and their incorporation into proteins associated with sporulation and (or) germination. If spore cAMP signalling is regulated by the relative concentrations of discadenine and autoactivator, and its disruption triggers the initiation of the spore germination cascade, then the accumulation of intracellular cAMP may be necessary for both sporulation and dormancy maintenance.

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Section: Prespore Cells Prestalk Cells and Tip Formationmentioning

confidence: 99%

Patterning of development in Dictyostelium discoideum: factors regulating growth, differentiation, spore dormancy, and germination

Cotter

Sands²,

Virdy³

et al. 1992

Biochem. Cell Biol.

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“…Although multiceilular aggregates of D. discoideum AX2 immersed in buffer require only exogenous SPIF to sustain the accumulation of SP60 protein synthesis (Wilkinson and Hames, 1985), when these cells are dissociated and kept dispersed in shaking suspension, SP60 protein synthesis ceases and fails to resume when cells are supplied with SPIF either alone or in combination with cAMP {Wilkinson and Hames, 1985). Here we show that this results from rapid degradation of SP60 mRNA upon disaggregation and that this mRNA is not maintained or re-accumulated when cells receive conditioned medium and/or cAMP.…”

Section: Discussionmentioning

confidence: 99%

Developmental expression and characterization of the gene encoding spore coat protein SP60 in Dictyostelium discoideum

Widdowson

Proffitt

Jagger

et al. 1990

Molecular Microbiology

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The complete coding sequence, upstream sequence and developmental expression of Dictyostelium discoideum AX2 spore coat protein gene SP60 is reported. The gene contains two exons, 154bp and 1121bp long, separated by a 119bp intron, and encodes a protein of 46,925 molecular weight plus a 23-amino-acid hydrophobic leader sequence. The N-terminus of the mature protein consists of four copies of a perfect hexapeptide repeat (GDWNNN). The central region is rich in cysteine residues, including four highly conserved cysteine-rich repeats with homology to 'EGF-like' repeats. The C-terminus is aspartate-rich and composed of multiple imperfect copies of a D(G/D)DYD repeat followed by several repeats of the tetrapeptide DNDW and derived sequences. A TATA box promoter motif juxtaposed to an oligo(dA) stretch lies 52bp upstream of the main transcriptional start site of the gene. Six AC-rich boxes occur in the region -327 to -556, all of which contain the consensus sequence CACAC. Two GC-rich boxes and a C-rich element (TTACCCCA) are also present upstream. Another open reading frame is positioned a short distance downstream of the SP60 gene in the opposite transcriptional orientation. Expression of the SP60 gene ceases upon disaggregation to single cells and cannot be restored by high levels of extracellular cAMP either alone or in combination with conditioned medium factors.

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“…Differentiation inducing factor (DIF) is a chlorinated alkyl hexanophenone required for the induction of certain prestalk‐specific genes (Berks and Kay, 1990). Other less well‐characterised factors include ammonia, adenosine, SDF‐1, SDF‐2, STIF, SPIF, and ψ‐factor (Wilkinson et al, 1985; So and Weeks, 1992; Soede et al, 1996; Anjard et al, 1997; Oohata et al, 1997; Yamada et al, 1997; Anjard et al, 1998). As discussed above, expression of the ecmA and ecmB genes defines a number of discrete prestalk cell populations, and it is known that the transcription of these genes is regulated through the concerted actions of cAMP and DIF‐1.…”

Section: Introductionmentioning

confidence: 99%

Identification of novel elements which regulate the cell-type specificity of Dictyostelium 7E gene expression

et al. 2001

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Spore coat protein synthesis during development of Dicytostelium discoideum requires a low-molecular-weight inducer and continued multicellularity

Cited by 31 publications

References 25 publications

Patterning of development in Dictyostelium discoideum: factors regulating growth, differentiation, spore dormancy, and germination

Patterning of development in Dictyostelium discoideum: factors regulating growth, differentiation, spore dormancy, and germination

Developmental expression and characterization of the gene encoding spore coat protein SP60 in Dictyostelium discoideum

Identification of novel elements which regulate the cell-type specificity of Dictyostelium 7E gene expression

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