Spore germination and ectomycorrhizae formation of Tricholoma matsutake on pine root systems with previously established ectomycorrhizae from a dikaryotic mycelial isolate of T. matsutake

Horimai, Yuka; Misawa, Hiroki; Suzuki, K.; Tateishi, Y.; Furukawa, Hitoshi; Yamanaka, Takashi; Yamashita, Shizuya; Takayama, Toshiharu; Fukuda, Masaki; Yamada, Akiyoshi

doi:10.1007/s00572-021-01028-3

Cited by 5 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shiro structures can be increased in size by one order of magnitude using larger culture vessels (~500 mL) or bottles containing 4-5 L soil, which require 2-3 y of incubation (Guerin-Laguette, 2021;Horimai et al, 2021). Horimai et al (2021) produced large shiro structures by transplanting an ectomycorrhizal seedling synthesized in vitro (~200 mL) into a larger culture vessel filled with 1 L soil under axenic conditions, and then into a culture bottle containing 4 L soil under non-axenic laboratory conditions. Kobayashi et al (2007) synthesized ectomycorrhizae in vitro in culture vessels containing 1 L soil, with a ca.…”

Section: Shiro Manipulation Under Controlled Environmental Conditionsmentioning

confidence: 99%

“…3A), supporting the competitive activation hypothesis and presenting a mechanism for the shiro genetic mosaic of shiro in situ. Thus, spore inoculation into ectomycorrhizal seedlings is a practical method for the effective production of large T. matsutake shiro structures on plant host root systems and to increase the genetic diversity of shiro mycelia (Horimai et al, 2021). This method apparently has a synergistic effect that stimulates competitive activation under environmental conditions.…”

Section: A D V a N C E P U B L I C A T I O Nmentioning

confidence: 99%

“…The identification of specific SSPs that determine the conifer hosts of T. matsutake and control gene expressions to influence host colonization levels would dramatically facilitate T. matsutake cultivation. The unique mycorrhizal structure of T. matsutake on its pine host, i.e., slender ectomycorrhizal root tips with a thin fungal mantle, carbonized root cortex in older ectomycorrhizae, elaborate branching that forms mycorrhizal clusters known as witch's brooms, and the mac-roscopic shiro structure require elucidation at the molecular level (Yamada et al, 1999a(Yamada et al, , 2006Horimai et al, 2021).…”

Section: Key Gaps In Our Understanding Of T Matsutake Biologymentioning

confidence: 99%

“…Single T. matsutake shiro structures can consist of several individuals (genets) of vegetative mycelia in situ ( Murata et al, 2005 ; Lian et al, 2006 ). Since shiro formations are mainly studied in terms of epigeous basidiomata along with limited data available about shiro mycelia in the soil ( Lian et al, 2006 ; Horimai et al, 2021 ), more comprehensive study is required for the structure of genetic heterogeneity and functional roles of sympatric genets to improve T. matsutake cultivation.…”

Section: Matsutake Cultivationmentioning

confidence: 99%

“…Mycorrhizal synthesis experiments in vitro generally use small culture vessels containing 100-200 mL soil or other substrate with or without added nutrients ( Yamada et al, 2006 ; Yamanaka, Maruyama, Yamada, Miyazaki, & Kikuchi, 2012 ; Murata et al, 2013a ; Yamanaka et al, 2014 ; Endo et al, 2015 ; Saito et al, 2018 ; Horimai et al, 2020 , 2021 ), which limit the volume of fully developed shiro structures in symbiosis with hosts to approximately 50 mL. Ectomycorrhizal systems can be established in even smaller culture vessels such as test tubes or Petri dishes to shorten the incubation period to a few mo; these are unsuitable for estimating shiro development despite the presence of measurable mycorrhizal root tips.…”

Section: Matsutake Cultivationmentioning

confidence: 99%

See 4 more Smart Citations

Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies

Yamada

2022

Mycoscience

Self Cite

View full text Add to dashboard Cite

Edible ectomycorrhizal mushrooms such as truffles (Tuber), chanterelles (Cantharellus cibarius Fr. and related species), porcinis (Boletus edulis Bull. and related species), saffron milk caps (Lactarius deliciosus [L.] Gray and related species), and matsutakes (Tricholoma matsutake [S. Ito & S. Imai] Singer and related species) have high economic value worldwide (Zambonelli & Bonito, 2012; Pérez-Moreno, Guerin-Laguette, Arzú, & Yu, 2020), with annual global sales estimated at several billion dollars. These mushrooms are harvested in forests or plantations for both domestic and export markets (Arora, 2008;Tsing, 2015, Pérez-Moreno et al., 2021. In Japan, imported matsutake mushrooms from countries such as China, USA, Canada, Morocco, Turkey, Mexico, and South Korea are valued at 80 to 100 million dollars annually (Aoki et al., 2022), which is equivalent to domestic T. matsutake production within Japan, mainly in Nagano and Iwate Prefectures (Yamanaka, . During the past 20 y, truffle imports to Japan have increased such that their economic value in Japan now equivalent to around 10% that of the matsutake mushroom import value (MAFF; https://www.maff.go.jp/e/index.html).To meet this increasing market demand, truffles have been harvested in tree plantations (Zambonelli, Iotti, & Murat, 2016). However, other edible mushrooms are largely harvested in forests because non-host and tree plantation cultivation techniques have not yet been established. Therefore, these mushrooms must be conserved in natural forests through controlled harvests or forest management to sustain moderate harvest levels for longer (decade-scale) periods (

show abstract

Section: Shiro Manipulation Under Controlled Environmental Conditionsmentioning

confidence: 99%

Section: A D V a N C E P U B L I C A T I O Nmentioning

confidence: 99%

Section: Key Gaps In Our Understanding Of T Matsutake Biologymentioning

confidence: 99%

Section: Matsutake Cultivationmentioning

confidence: 99%

Section: Matsutake Cultivationmentioning

confidence: 99%

See 3 more Smart Citations

Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies

Yamada

2022

Mycoscience

Self Cite

View full text Add to dashboard Cite

show abstract

Extraction, Structure and Bioactivity of Polysaccharides from Tricholoma matsutake (S. Ito et Imai) Singer (Review)

Wang¹,

Li²,

Wang³

et al. 2022

Appl Biochem Microbiol

View full text Add to dashboard Cite

Biodiversity of Tricholoma matsutake (syn. T. nauseosum) and its related species based on repetitive DNA and genomics

Murata

Yamada

Ichida

et al. 2023

Botany

View full text Add to dashboard Cite

Tricholoma matsutake and its related species are ectomycorrhizal Agaricomycetes that produce prized mushrooms collectively called “matsutake.” These mushrooms are mainly distributed in the Northern Hemisphere. In this review, we describe both the pioneering work and recent advances in our understanding of the distribution and evolution of matsutake, with a special focus on genomic studies. DNA sequence analyses revealed that T. caligatum was the earliest species within this group, followed by T. fulvocastaneum, the cluster with T. ilkkae, T. dulciolens, and T. bakamatsutake, and finally the cluster with T. murrillianum, T. mesoamericanum, T. anatolicum, and T. matsutake. Although analyses based on mobile DNAs and whole-genome sequences revealed a similar clustering pattern, there are distinct differences in the distribution of mobile DNAs and genomic structure of T. bakamatsutake and T. matsutake. Furthermore, repetitive DNA can be used as markers to distinguish among strains and populations of T. matsutake from different geographical regions, including identifying dispersals of basidiospores. “Telomere-to-telomere genome sequencing” analyses unearthed that both T. matsutake and T. bakamatsutake underwent explosive multiplications of retrotransposons within their genomes but with different mobile DNA elements expanded between them. Contrastingly, the structure of the mating loci between T. matsutake and T. bakamatsutake is highly conserved.

show abstract

Spore germination and ectomycorrhizae formation of Tricholoma matsutake on pine root systems with previously established ectomycorrhizae from a dikaryotic mycelial isolate of T. matsutake

Cited by 5 publications

References 35 publications

Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations <i>in vitro</i> and efficient production of fruiting bodies

Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations <i>in vitro</i> and efficient production of fruiting bodies

Extraction, Structure and Bioactivity of Polysaccharides from Tricholoma matsutake (S. Ito et Imai) Singer (Review)

Biodiversity of Tricholoma matsutake (syn. T. nauseosum) and its related species based on repetitive DNA and genomics

Contact Info

Product

Resources

About