Spore resistance and ultra heat treatment processes

Brown, Kenneth L.

doi:10.1111/j.1365-2672.1994.tb04359.x

Cited by 36 publications

(23 citation statements)

References 65 publications

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“…The metabolic dormancy (Setlow, 1994) and the resistance properties of bacterial spores are both crucial to the ecological role of spores as survival structures (Brown, 1994).…”

Section: Discussionmentioning

confidence: 99%

Experimental Effect of Ozone upon Some Indicator Bacteria for Preservation of an Ecologically Protected Watery System

Voidarou

Tzora

Skoufos

et al. 2006

Water Air Soil Pollut

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In the frame of a project concerning the evaluation of the pollution level and the ozone experimental effect upon some bacterial indicators of a protected ecosystem, water samples were estimated microbiologically before and after saturation with ozone gas for 5, 10, 15, 20 and 30 min. We found that the ozone treatment decreased the concentration in all different bacterial indicators by reducing their number for more than six times after 15 min of application. Total inactivation was observed for C. perfringens vegetative cells. Since there are complex relations between the microbial loading the water characteristics and the action of ozone, further studies are needed to determine the optimum conditions of ozone effect, as its use consists of a promising technology.

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“…The metabolic dormancy (Setlow, 1994) and the resistance properties of bacterial spores are both crucial to the ecological role of spores as survival structures (Brown, 1994).…”

Section: Discussionmentioning

confidence: 99%

Experimental Effect of Ozone upon Some Indicator Bacteria for Preservation of an Ecologically Protected Watery System

Voidarou

Tzora

Skoufos

et al. 2006

Water Air Soil Pollut

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“…Spores are formed if bacteria experience environmental conditions that are deleterious to growth, such as nutrient limitation, and when conditions are again suitable for growth, the spore germinates to form a vegetative cell that begins to proliferate (Cook & Pierson, 1983;Santo & Doi, 1974). As spores are highly resistant to heat sterilization and to various drugs, control of spores of food poisoning bacteria, such as Clostridium botulinum (Abgueguen et al, 2003), Clostridium perfringens (Valenzuela-Martinez et al, 2010), and Bacillus cereus (Granum & Lund, 1997), and food and beverage spoilage bacteria, such as Bacillus coagulans (Cosentino, Mulargia, Pisano, Tuveri, & Palmas, 1997;Palop, Raso, Pagán, Condón, & Sala, 1999) and Moorella thermoacetica (Prevost, Andre, & Remize, 2010), is an extremely important problem in food and beverage preservation (Brown, 1994).…”

Section: Introductionmentioning

confidence: 99%

The mechanisms and effect of epigallocatechin gallate (EGCg) on the germination and proliferation of bacterial spores

et al. 2012

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“…Endospore heat resistance and the ability to germinate within minutes to form vegetative cells is of tremendous practical importance for the food processing industry (Brown 1994). Spores in the dormant state are not hazardous.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the role of bacterial endospore cortex structure in resistance properties and demonstration of its conservation amongst species

Atrih

Foster

2001

J Appl Microbiol

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Aims: The aim of this work was to compare the chemical structure of the spore cortex of a range of species, and to determine any correlation between cortex structure and spore resistance properties. Methods and Results: The ®ne chemical structure of the cortex of Bacillus subtilis, Bacillus megaterium, Bacillus cereus and Clostridium botulinum was examined by muropeptide analysis using reverse phase HPLC. There is a conserved basic structure between peptidoglycan of these species, with the only difference being the level of de-N-acetylation of an amino sugar. In order to determine if an alteration in cortex structure correlates with heat resistance properties, the peptidoglycan structure and properties of B. subtilis spores prepared under different conditions were compared. Peptidoglycan from spores prepared in Nutrient Broth (NB) showed reduction in single L L-alanine substituted muramic acid to only 13á9% compared with 20á6% in CCY-grown spores. NB-prepared spores are also unstable, with 161-fold less heat resistance (60 min, 85°C) and 43 times less Mn 2+ content than CCY-grown spores. Addition of MnCl 2 to NB led to a peptidoglycan pro®le similar to CCY-grown spores, sevenfold more heat resistance (60 min, 85°C) and an 86-fold increase in Mn 2+ content. Addition of CCY salts to NB led all parameters to be comparable with CCY-grown spore levels. Conclusions: It has been shown that peptidoglycan structure is conserved in four sporeforming bacteria. Also, spore heat resistance is multifactorial and cannot be accounted for by any single parameter. Signi®cance and Impact of the Study: Endospores made by diverse species most likely have common mechanisms of heat resistance. However, the molecular basis for their resistance remains elusive.

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Spore resistance and ultra heat treatment processes

Cited by 36 publications

References 65 publications

Experimental Effect of Ozone upon Some Indicator Bacteria for Preservation of an Ecologically Protected Watery System

Experimental Effect of Ozone upon Some Indicator Bacteria for Preservation of an Ecologically Protected Watery System

The mechanisms and effect of epigallocatechin gallate (EGCg) on the germination and proliferation of bacterial spores

Analysis of the role of bacterial endospore cortex structure in resistance properties and demonstration of its conservation amongst species

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