Spores ofMucor ramosissimus, Mucor plumbeusandMucor circinelloidesand their ability to activate human complement systemin vitro

Granja, Luiz Fernando Zmetek; Pinto, Lysianne; Almeida, Carlos Eduardo; Alviano, Daniela Sales; Silva, Maria Helena da; Ejzemberg, Regina; Alviano, Celuta Sales

doi:10.3109/13693780903096669

Cited by 11 publications

(9 citation statements)

References 35 publications

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“…However, Rhizopus species, which are responsible for almost 80% of infections, remain as the major cause of most mucormycosis cases. Also, a considerable number of mucormycosis cases have been associated with M. circinelloides [31]. …”

Section: Discussionmentioning

confidence: 99%

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Identification of Mucorales isolates from soil using morphological and molecular methods

et al. 2016

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Background and Purpose:Soil is the main habitat of saprophytic and pathogenic fungi. Mucoromycotina constitutes a large group of soil fungi, with certain opportunistic members causing systemic infections in immunocompromised hosts. The majority of human and animal infections are caused by the members of the genera Rhizopus, Mucor, Rhizomucor, Lichtheimia (Absidia), Cunninghamella, and Mortierella. Accordingly, in the present study, we aimed to isolate and identify the main genera of the order Mucorales, using molecular assays and morphological features.Materials and Methods:In total, 340 soil samples were collected from seven public parks throughout the city and sidewalk gardens in 14 municipal districts in Isfahan, Iran. All the samples were cultured on the appropriate media, incubated at 27°C for 2- 4 days, and examined daily for visible fungal growth. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was applied and macroscopic, microscopic, and physiological characteristics were assessed to identify fungal colonies.Results:400 pure colonies, belonging to the orders Mucorales and Mortierellales, including the genera Lichtheimia, Rhizopus, Rhizomucor, Mucor, Cunninghamella, and Mortierella, were identified. The genus Rhizopus (35.5%) was the most frequent isolate, followed by Mucor (32.25%) and Rhizomucor (27.5%).Conclusion:The results emphasize the importance of opportunistic fungi in public areas and indicate the risk of exposure for immunocompromised individuals.

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Section: Discussionmentioning

confidence: 99%

“…Although M. plumbeus is extensively used for research purposes in biotransformation of natural products, no cases of mycosis have been so far linked to this species [31]. …”

Section: Discussionmentioning

confidence: 99%

Identification of Mucorales isolates from soil using morphological and molecular methods

et al. 2016

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“…There have been a few mucormycosis cases associated with M. ramosissimus . However, no mycosis cases associated with M. plumbeus have yet been reported ( 32 ). In this study, the genera of Mucor and Lichtheimia were represented in 29.17% and 10% of the 120 pure Mucorales cultures, respectively.…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification of Mucor and Lichtheimia Species in Pure Cultures of Zygomycetes

Ziaee

Zia

Bayat

et al. 2016

Jundishapur J Microbiol

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BackgroundThe Mucorales are an important opportunistic fungi that can cause mucormycosis in immunocompromised patients. The fast and precise diagnosis of mucormycosis is very important because, if the diagnosis is not made early enough, dissemination often occurs. It is now well established that molecular methods such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) are feasible and reliable tools for the early and accurate diagnosis of mucormycosis agents.ObjectivesThe present study was conducted to evaluate the validity of PCR-RFLP for the identification of Mucorales and some important Mucor and Lichtheimia species in pure cultures of Zygomycetes.Materials and MethodsSpecific sense and anti-sense primers were used to amplify the Mucorales, Mucor, and Lichtheimia DNA. The PCR products were digested by AfIII, XmnI, and AcII restriction enzymes, and the resultant restriction pattern was analyzed.ResultsOn the basis of the molecular and morphological data, we identified Mucor plumbeus (10.83%), M. circinelloides (9.17%), Lichtheimia corymbifera (9.17%), M. racemosus (5.83%), M. ramosissimus (3.33%), and L. blakesleeana (0.83%).ConclusionsIt seems that PCR-RFLP is a suitable technique for the identification of Mucorales at the species level.

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“…The samples were incubated with absorbed serum in either GVB ++ , GVB-Mg-EGTA, or GVB-EDTA. After 60 min at 37°C, the samples were centrifuged at 1500 × g under refrigeration and the supernatants were collected and residual complement was measured in a spectrophotometer (Beckman Coulter, Brea, CA, USA) at 540 nm using the haemolytic system ( Granja et al 2010 ). Melanin and melanin ghost samples sensitised with guinea pig anti-melanin antibodies (1/200 dilution, previously titrated) were subjected to the same activation process but without the addition of chelators.…”

Section: Methodsmentioning

confidence: 99%

“…Numerous fungi such as Aspergillus fumigatus , Candida albicans , C. neoformans , and Mucor spp activate the complement system, mainly by the alternative pathway ( Speth et al 2008 , Granja et al 2010 ). Wünthrich et al (2015) demonstrated that dectin-2 (and to a lesser extent dectin-1)-mediated recognition of F. pedrosoi is largely responsible for the development of Ag-specific Th17 cells, while Mincle appears to inhibit the development of this T-helper subset.…”

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confidence: 99%

Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system

Pinto

Granja

Almeida

et al. 2018

Mem. Inst. Oswaldo Cruz

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BACKGROUNDMelanin production has been associated with virulence in various pathogenic fungi, including Fonsecaea pedrosoi, the major etiological agent for chromoblastomycosis, a subcutaneous fungal disease that occurs in South America.OBJECTIVEThe aim of this study was to evaluate the effects of acid-basic extracted F. pedrosoi melanin particles and fungal cell ghosts obtained by Novozym 234 treatment on their ability to activate the human complement system.METHODSThe ability of melanin particles and fungal cell ghosts to activate the human complement system was evaluated by complement consumption, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA).FINDINGSUnsensitised melanin particles and melanin ghosts presented complement consumption of 82.67 ± 2.08% and 96.04 ± 1.13%, respectively. Immunofluorescence assays revealed intense deposition of the C3 and C4 fragments on the surface of melanin particles and ghosts extracted from F. pedrosoi. Deposition of the C3, C4, and C5 fragments onto melanin samples and zymosan was confirmed by ELISA. Deposition of small amounts of C1q and C9 onto melanin samples and zymosan was detected by ELISA.CONCLUSION Fonsecaea pedrosoi melanin particles and fungal cell ghosts activated the complement system mainly through an alternative pathway.

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Spores ofMucor ramosissimus, Mucor plumbeusandMucor circinelloidesand their ability to activate human complement systemin vitro

Cited by 11 publications

References 35 publications

Identification of Mucorales isolates from soil using morphological and molecular methods

Identification of Mucorales isolates from soil using morphological and molecular methods

Molecular Identification of Mucor and Lichtheimia Species in Pure Cultures of Zygomycetes

Melanin particles isolated from the fungus Fonsecaea pedrosoi activates the human complement system

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