Sporicidal Characteristics of Heated Dolomite Powder against Bacillus subtilis Spores

Yasue, Syogo; Sawai, Jun; Kikuchi, Mikio; Nakakuki, Takahito; Sano, Kazuo; Kikuchi, Takahide

doi:10.4265/bio.19.113

Cited by 9 publications

(4 citation statements)

References 26 publications

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“…There are very few studies on the effect of calcium preparation against resistive bacterial spores Sawai et al, 2003;2007. Yasue et al 2014 investigated the sporicidal effects of heated dolomite against Bacillus subtilis spores.…”

Section: Sporicidal Activity Of Hespmentioning

confidence: 99%

Antimicrobial Characteristics of Heated Eggshell Powder

et al. 2015

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Eggshells have high bioavailability and can be used as a source of calcium. The main component is CaCO 3 , which, when heated, is converted to CaO. Seashells are also mainly composed of CaCO 3 and were previously found to exhibit antimicrobial activity after being heated. In this study, heated eggshell powder HESP was found to have antimicrobial activity against bacterial vegetative cells, fungi and bacterial spores. Parameters, such as the minimum inhibitory concentration, were determined with kinetic analysis using an indirect conductimetric assay. Moreover, HESP was able to kill the Bacillus subtilis spores. There were no significant differences in the activity between HESP, heated scallop-shell powder and pure CaO. The MIC values for HESP against bacteria and fungi were 0.29-0.43 and 1.3-1.5 mg/mL, respectively. Against B. subtilis spores, a reduction of two orders of magnitude of viability was confirmed following 20 min of treatment at 10 mg/mL at 60 . The active oxygen generated from the HESP slurry was examined with chemiluminescence. The intensity of this increased with increasing concentrations of the HESP slurry. This suggests that HESP could be used as a natural antimicrobial agent. Although a high pH is the main contributor to this antimicrobial activity, active oxygen species generated from HESP are likely to be the main antimicrobial agents..

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Section: Sporicidal Activity Of Hespmentioning

confidence: 99%

Antimicrobial Characteristics of Heated Eggshell Powder

et al. 2015

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“…In addition to shells, eggshells [53], and dolomite [54] are mainly composed of CaCO 3 . Naturally, their composition can be changed to CaO by calcination to confer antimicrobial activity upon them.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity and Transparency of Polyvinyl Butyral Paint Containing Heated Scallop-Shell Powder

et al. 2023

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The main component of seashells is calcium carbonate (CaCO3). When seashells are calcined at high temperatures, CaCO3 becomes calcium oxide (CaO), and this CaO exhibits antimicrobial activity. In this study, we attempted to develop a transparent coating that retains antimicrobial activity for a long time by mixing polyvinyl butyral (PVB), which has excellent adhesive and binding properties, with heated shell powder (HSP). The scallop HSPs used in this study were nanoparticles with a particle diameter of approximately several hundred nm, and the prepared paint showed high transparency. Elemental analysis showed that scallop HSP particles existed in the paint as Ca(OH)2. The antimicrobial activity of the surface applied with scallop HSP-containing PVB (HSSP-PVB) paint was then evaluated using JIS Z 2801 and ISO 21702: 2019. The HSSP-PVB paint-applied surfaces showed high antibacterial and antiviral activity. The antimicrobial activity of the scallop HSP-PVB paint-applied surface was attributed to the creation of a strongly alkaline environment due to surface hydration, and the strongly alkaline environment was maintained for a long period of time. It was suggested that the PVB covered the surface of the scalloped HSP particles, which significantly prevented the HSP from contacting CO2 and H2O molecules in the air.

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“…Dolomite is double salt composed of calcium carbonate (CaCO 3 ) and magnesium carbonate (MgCO 3 ). The heat treatment of CaCO 3 and MgCO 3 respectively generates calcium oxide (CaO) and magnesium oxide (MgO), which have antimicrobial activity [18] and antiviral (H3N3 influenza virus) [19]. In plant growth and development, calcium plays a vital role in cell membranes' structure, while magnesium is an essential factor that makes up the chlorophyll [20].…”

Section: Introductionmentioning

confidence: 99%

Growth, secondary metabolite production, and in vitro antiplasmodial activity of Sonchus arvensis L. callus under dolomite [CaMg(CO3)2] treatment

et al. 2021

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Malaria is still a global health problem. Plasmodium is a single-cell protozoan parasite that causes malaria and is transmitted to humans through the female Anopheles mosquito. The previous study showed that Sonchus arvensis L. callus has antiplasmodial activity. Several treatments are needed for callus quality improvement for antimalarial compound production. This study aimed to examine the effect of dolomite [CaMg(CO3)2] on growth (morpho-anatomical structure and biomass), secondary metabolite production, and in vitro antiplasmodial activity of S. arvensis L. callus. In this study, leaf explants were grown in Murashige and Skoog medium with a combination of 2,4-dichlorophenoxyacetic acid (2,4-D, one mg/L) and 6-benzyl amino purine (BAP, 0.5 mg/L) with dolomite (50, 75, 100, 150, and 200 mg/L). The 21 days callus ethanolic and methanolic extract were analyzed by gas chromatography-mass spectrometry (GC-MS) and thin-layer chromatography (TLC). The antiplasmodial test was performed on a blood culture infected with Plasmodium falciparum strain 3D7 using the Rieckmann method. The results showed that dolomite significantly affected callus growth, metabolite profile, and in vitro antiplasmodial activity. Dolomite (150 mg/L) showed the highest biomass (0.590 ± 0.136 g fresh weight and 0.074 ± 0.008 g dry weight). GC-MS analysis detected four compounds from callus ethanolic extract. Pelargonic acid, decanoic acid, and hexadecanoic acid were major compounds. One new terpenoid compound is based on TLC analysis. S. arvensis L. callus has antiplasmodial activity with the IC50 value of 5.037 μg/mL. It was three times lower than leaf methanolic extract and five times lower than leaf ethanolic extract.

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Sporicidal Characteristics of Heated Dolomite Powder against Bacillus subtilis Spores

Cited by 9 publications

References 26 publications

Antimicrobial Characteristics of Heated Eggshell Powder

Antimicrobial Characteristics of Heated Eggshell Powder

Antimicrobial Activity and Transparency of Polyvinyl Butyral Paint Containing Heated Scallop-Shell Powder

Growth, secondary metabolite production, and in vitro antiplasmodial activity of Sonchus arvensis L. callus under dolomite [CaMg(CO3)2] treatment

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